Match!
Prashant Mali
University of California, San Diego
81Publications
29H-index
11.1kCitations
Publications 81
Newest
#1Ana M. Moreno (UCSD: University of California, San Diego)H-Index: 3
#2Nathan D. Palmer (UCSD: University of California, San Diego)H-Index: 1
Last.Prashant Mali (UCSD: University of California, San Diego)H-Index: 29
view all 9 authors...
Protein-based therapeutics can activate the adaptive immune system, leading to the production of neutralizing antibodies and the clearance of the treated cells mediated by cytotoxic T cells. Here, we show that the sequential use of immune-orthogonal orthologues of CRISPR-associated protein 9 (Cas9) and adeno-associated viruses (AAVs) evades adaptive immune responses and enables effective gene editing using repeated dosing. We compared total sequence similarities and predicted binding strengths t...
#1Ana M. Moreno (UCSD: University of California, San Diego)H-Index: 3
#2Nathan D. Palmer (UCSD: University of California, San Diego)H-Index: 1
Last.Prashant Mali (UCSD: University of California, San Diego)H-Index: 29
view all 9 authors...
#1Ana M. Moreno (UCSD: University of California, San Diego)H-Index: 3
#2Glaucilene F Catroli (UCSD: University of California, San Diego)
Last.Prashant Mali (UCSD: University of California, San Diego)H-Index: 29
view all 8 authors...
Current treatments for chronic pain rely largely on opioids despite their unwanted side effects and risk of addiction. Genetic studies have identified in humans key targets pivotal to nociceptive processing, with the voltage-gated sodium channel, NaV1.7 (SCN9A), being perhaps the most promising candidate for analgesic drug development. Specifically, a hereditary loss-of-function mutation in NaV1.7 leads to insensitivity to pain without other neurodevelopmental alterations. However, the high sequ...
#1Prashant Mali (UCSD: University of California, San Diego)H-Index: 29
Transcriptome targeting offers a tunable and reversible approach for cellular engineering. Accordingly, the ability to precisely perturb RNAs has broad implications for research and therapeutics. In this issue, Rauch and colleagues present a new addition to the RNA engineering toolbox that is modular, versatile, and built using human components.
#1Genghao Chen (UCSD: University of California, San Diego)H-Index: 2
#2Dhruva Katrekar (UCSD: University of California, San Diego)H-Index: 3
Last.Prashant Mali (UCSD: University of California, San Diego)H-Index: 29
view all 3 authors...
Targeted transcriptome engineering, in contrast to genome engineering, offers a complementary and potentially tunable and reversible strategy for cellular engineering. In this regard, adenosine to inosine (A-to-I) RNA base editing was recently engineered to make programmable base conversions on target RNAs. Similar to the DNA base editing technology, A-to-I RNA editing may offer an attractive alternative in a therapeutic setting, especially for the correction of point mutations. This Perspective...
#1Dhruva Katrekar (UCSD: University of California, San Diego)H-Index: 3
#2Genghao Chen (UCSD: University of California, San Diego)H-Index: 2
Last.Prashant Mali (UCSD: University of California, San Diego)H-Index: 29
view all 8 authors...
We present in vivo sequence-specific RNA base editing via adenosine deaminases acting on RNA (ADAR) enzymes with associated ADAR guide RNAs (adRNAs). To achieve this, we systematically engineered adRNAs to harness ADARs, and comprehensively evaluated the specificity and activity of the toolsets in vitro and in vivo via two mouse models of human disease. We anticipate that this platform will enable tunable and reversible engineering of cellular RNAs for diverse applications.
#1Anagha Deshpande (DI: Discovery Institute)H-Index: 3
#2Bo Rui Chen (DI: Discovery Institute)
Last.Aniruddha J. Deshpande (DI: Discovery Institute)
view all 8 authors...
Gene perturbation studies have been extensively used to investigate the role of individual genes in AML pathogenesis. For achieving complete gene disruption, many of these studies have made use of complex gene knockout models. While these studies with knockout mice offer an elegant and time-tested system for investigating genotype-to-phenotype relationships, a rapid and scalable method for assessing candidate genes that play a role in AML cell proliferation or survival in AML models will help ac...
#1Kyle Ford (UCSD: University of California, San Diego)H-Index: 1
#2Daniella McDonald (UCSD: University of California, San Diego)H-Index: 1
Last.Prashant Mali (UCSD: University of California, San Diego)H-Index: 29
view all 3 authors...
Abstract RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas proteins have recently emerged as versatile tools to investigate and engineer the genome. The programmability of CRISPR–Cas has proven especially useful for probing genomic function in high-throughput. Facile single-guide RNA library synthesis allows CRISPR–Cas screening to rapidly investigate the functional consequences of genomic, transcriptomic, and epigenomic perturbations. Furthermore, by co...
#1Dhruva Katrekar (UCSD: University of California, San Diego)H-Index: 3
#2Ana M. Moreno (UCSD: University of California, San Diego)H-Index: 3
Last.Prashant Mali (UCSD: University of California, San Diego)H-Index: 29
view all 5 authors...
Recombinant adeno-associated viruses (AAVs) are among the most commonly used vehicles for in vivo gene delivery. However, their tropism is limited, and additionally their efficacy can be negatively affected by prevalence of neutralizing antibodies in sera. Methodologies to systematically engineer AAV capsid properties would thus be of great relevance. In this regard, we develop here multi-functional AAVs by engineering precision tethering of oligonucleotides onto the AAV surface, and thereby ena...
#1Michael Hu (UCSD: University of California, San Diego)H-Index: 1
#2Amir Dailamy (UCSD: University of California, San Diego)
Last.Prashant Mali (UCSD: University of California, San Diego)H-Index: 29
view all 7 authors...
123456789