Luhan Yang
Harvard University
30Publications
16H-index
7,049Citations
Publications 30
Newest
Published on Apr 19, 2018in bioRxiv
Henrik D. Møller9
Estimated H-index: 9
(University of Copenhagen),
Lin Lin14
Estimated H-index: 14
(Aarhus University)
+ 14 AuthorsXin Liu33
Estimated H-index: 33
(Beijing Genomics Institute)
Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders and diseases such as cancer. However, there is a lack of genetic engineering tool to recapitulate these features. Here, we report the discovery that delivery of pairs of CRISPR/Cas9 guide RNAs into human cells generate functional eccDNAs and ring chromosomes. We generated a dual-fluorescence eccDNA biosensor system, which allows us to study CRISPR deletion, inversion, and ci...
Source Cite
Published on Aug 24, 2018in Nucleic Acids Research 11.56
Henrik Devitt Møller1
Estimated H-index: 1
(Aarhus University),
Lin Lin14
Estimated H-index: 14
(Aarhus University)
+ 14 AuthorsXin Liu33
Estimated H-index: 33
5 Citations Source Cite
Published on Sep 22, 2017in Science 41.06
Dong Niu3
Estimated H-index: 3
(Zhejiang University),
Hong-Jiang Wei4
Estimated H-index: 4
(Yunnan Agricultural University)
+ 19 AuthorsEllen Shrock4
Estimated H-index: 4
(Harvard University)
Xenotransplantation is a promising strategy to alleviate the shortage of organs for human transplantation. In addition to the concerns about pig-to-human immunological compatibility, the risk of cross-species transmission of porcine endogenous retroviruses (PERVs) has impeded the clinical application of this approach. We previously demonstrated the feasibility of inactivating PERV activity in an immortalized pig cell line. We now confirm that PERVs infect human cells, and we observe the horizont...
106 Citations Source Cite
Published on Oct 9, 2017in Nature Communications 12.35
Luhan Yang16
Estimated H-index: 16
,
Adrian W. Briggs17
Estimated H-index: 17
+ 10 AuthorsEmal Lesha4
Estimated H-index: 4
1 Citations Source Cite
Published on Jan 1, 2017in Nature Protocols 12.42
Gang Wang11
Estimated H-index: 11
,
Luhan Yang16
Estimated H-index: 16
+ 7 AuthorsWilliam T. Pu56
Estimated H-index: 56
This protocol uses a doxycycline-inducible Cas9 transgene carried on a piggyBac transposon for robust, highly efficient and scarless genome editing of human iPSCs, enabling a parental line to be engineered to harbor many separate mutations.
27 Citations Source Cite
Published on Nov 1, 2017in Xenotransplantation 4.72
Marc Güell14
Estimated H-index: 14
,
Dong Niu3
Estimated H-index: 3
(Zhejiang University)
+ 6 AuthorsLuhan Yang16
Estimated H-index: 16
12 Citations Source Cite
Published on Jul 28, 2016in bioRxiv
Luhan Yang16
Estimated H-index: 16
(Harvard University),
Adrian W. Briggs6
Estimated H-index: 6
(Harvard University)
+ 10 AuthorsEmal Lesha4
Estimated H-index: 4
(Harvard University)
Precise genetic modifications are essential for biomedical research and gene therapy. Yet, traditional homology-directed genome editing is limited by the requirements for DNA cleavage, donor DNA template and the endogenous DNA break-repair machinery. Here we present programmable cytidine deaminases that enable site-specific cytidine to thymidine (C-to-T) genomic edits without the need for DNA cleavage. Our targeted deaminases are efficient and specific in Escherichia coli, converting a genomic C...
4 Citations Source Cite
Published on Jul 8, 2016in Science 41.06
Jef D. Boeke87
Estimated H-index: 87
,
George M Church G M132
Estimated H-index: 132
+ 22 AuthorsLiam J. Holt13
Estimated H-index: 13
The Human Genome Project (“HGP-read”), nominally completed in 2004, aimed to sequence the human genome and to improve the technology, cost, and quality of DNA sequencing ( 1 , 2 ). It was biology's first genome-scale project and at the time was considered controversial by some. Now, it is recognized as one of the great feats of exploration, one that has revolutionized science and medicine.
67 Citations Source Cite
Published on Dec 1, 2016in Nature Communications 12.35
Luhan Yang16
Estimated H-index: 16
,
Adrian W. Briggs17
Estimated H-index: 17
+ 10 AuthorsEmal Lesha4
Estimated H-index: 4
Precision genome engineering using homology donors and the endogenous DNA break repair machinery and recently CRISPR-Cas9 targeted APOBECs have been demonstrated. Here the authors design zinc-finger and TALE chimeric deaminases and investigate editing efficiency and off-target effects.
31 Citations Source Cite
Published on Nov 1, 2016in Nature 41.58
Luhan Yang16
Estimated H-index: 16
,
Adrian W. Briggs17
Estimated H-index: 17
+ 10 AuthorsDaniel B. Goodman9
Estimated H-index: 9
123