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Luhan Yang
Harvard University
26Publications
15H-index
7,229Citations
Publications 26
Newest
Published on Aug 24, 2018in Nucleic Acids Research 11.56
Henrik Devitt Møller10
Estimated H-index: 10
(University of Copenhagen),
Lin Lin15
Estimated H-index: 15
(Aarhus University)
+ 14 AuthorsXin Liu32
Estimated H-index: 32
12 Citations Source Cite
Published on Apr 19, 2018in bioRxiv
Henrik Devitt Møller10
Estimated H-index: 10
(University of Copenhagen),
Lin Lin15
Estimated H-index: 15
(Aarhus University)
+ 14 AuthorsXin Liu32
Estimated H-index: 32
(Beijing Genomics Institute)
Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders and diseases such as cancer. However, there is a lack of genetic engineering tool to recapitulate these features. Here, we report the discovery that delivery of pairs of CRISPR/Cas9 guide RNAs into human cells generate functional eccDNAs and ring chromosomes. We generated a dual-fluorescence eccDNA biosensor system, which allows us to study CRISPR deletion, inversion, and ci...
Source Cite
Published on Dec 1, 2017in Nature Communications 12.35
Luhan Yang15
Estimated H-index: 15
,
Adrian W. Briggs18
Estimated H-index: 18
+ 10 AuthorsEmal Lesha4
Estimated H-index: 4
1 Citations Source Cite
Published on Nov 1, 2017in Xenotransplantation 4.72
Marc Güell15
Estimated H-index: 15
,
Dong Niu3
Estimated H-index: 3
(Zhejiang University)
+ 6 AuthorsLuhan Yang15
Estimated H-index: 15
13 Citations Source Cite
Published on Sep 22, 2017in Science 41.06
Dong Niu3
Estimated H-index: 3
(Zhejiang University),
Hong-Jiang Wei4
Estimated H-index: 4
(Yunnan Agricultural University)
+ 19 AuthorsEllen Shrock5
Estimated H-index: 5
(Harvard University)
Xenotransplantation is a promising strategy to alleviate the shortage of organs for human transplantation. In addition to the concerns about pig-to-human immunological compatibility, the risk of cross-species transmission of porcine endogenous retroviruses (PERVs) has impeded the clinical application of this approach. We previously demonstrated the feasibility of inactivating PERV activity in an immortalized pig cell line. We now confirm that PERVs infect human cells, and we observe the horizont...
128 Citations Source Cite
Published on Feb 2, 2017
George M Church G M135
Estimated H-index: 135
,
Luhan Yang1
Estimated H-index: 1
,
Margo R. Monroe
L'invention concerne des procedes et des compositions de modification de l'ADN mitochondrial d'une cellule eucaryote a l'aide d'un ou plusieurs virus adeno-associes mitochondriaux specifiques pour administrer un ou plusieurs acides nucleiques codant pour un systeme CRISPR notamment une proteine Cas9 ou sa variante inactive par rapport a une nuclease et un ARN guide dans une mitochondrie pour l'expression a l'interieur des mitochondries. Le systeme Cas9 peut couper, casser ou reguler un acide nuc...
Published on Jan 1, 2017in Nature Protocols 12.42
Gang Wang39
Estimated H-index: 39
,
Luhan Yang15
Estimated H-index: 15
+ 7 AuthorsWilliam T. Pu57
Estimated H-index: 57
This protocol uses a doxycycline-inducible Cas9 transgene carried on a piggyBac transposon for robust, highly efficient and scarless genome editing of human iPSCs, enabling a parental line to be engineered to harbor many separate mutations.
32 Citations Source Cite
Published on Dec 1, 2016in Nature Communications 12.35
Luhan Yang15
Estimated H-index: 15
,
Adrian W. Briggs18
Estimated H-index: 18
+ 10 AuthorsEmal Lesha4
Estimated H-index: 4
Precision genome engineering using homology donors and the endogenous DNA break repair machinery and recently CRISPR-Cas9 targeted APOBECs have been demonstrated. Here the authors design zinc-finger and TALE chimeric deaminases and investigate editing efficiency and off-target effects.
33 Citations Source Cite
Published on Nov 1, 2016in Nature 41.58
Luhan Yang15
Estimated H-index: 15
,
Adrian W. Briggs18
Estimated H-index: 18
+ 10 AuthorsDaniel B. Goodman9
Estimated H-index: 9
Published on Oct 7, 2016
George M Church G M135
Estimated H-index: 135
,
Luhan Yang1
Estimated H-index: 1
,
Marc Guell
La presente invention concerne un procede de modulation d'une partie ou de toutes les copies d'un gene dans une cellule incluant l'introduction, dans une cellule, d'une ou plusieurs sequences d'acides ribonucleiques (ARN) qui comprennent une partie qui est complementaire de l'ensemble ou d'une partie de la ou des multiples sequences d'acides nucleiques cibles, et une sequence d'acides nucleiques qui code pour une proteine Cas et le maintien des cellules dans des conditions dans lesquelles la pro...
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