Luhan Yang
Harvard University
Publications 26
#1Henrik Devitt Møller (UCPH: University of Copenhagen)H-Index: 10
#2Lin Lin (AU: Aarhus University)H-Index: 15
Last.Xiuqing Zhang (CAS: Chinese Academy of Sciences)H-Index: 52
view all 17 authors...
#1Henrik Devitt Møller (UCPH: University of Copenhagen)H-Index: 10
#2Lin Lin (AU: Aarhus University)H-Index: 15
Last.Xin Liu (Beijing Genomics Institute)H-Index: 32
view all 17 authors...
Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders and diseases such as cancer. However, there is a lack of genetic engineering tool to recapitulate these features. Here, we report the discovery that delivery of pairs of CRISPR/Cas9 guide RNAs into human cells generate functional eccDNAs and ring chromosomes. We generated a dual-fluorescence eccDNA biosensor system, which allows us to study CRISPR deletion, inversion, and ci...
#1Dong Niu (ZJU: Zhejiang University)H-Index: 3
#2Hong-Jiang Wei (Yunnan Agricultural University)H-Index: 4
Last.Ellen Shrock (Harvard University)H-Index: 5
view all 22 authors...
Xenotransplantation is a promising strategy to alleviate the shortage of organs for human transplantation. In addition to the concerns about pig-to-human immunological compatibility, the risk of cross-species transmission of porcine endogenous retroviruses (PERVs) has impeded the clinical application of this approach. We previously demonstrated the feasibility of inactivating PERV activity in an immortalized pig cell line. We now confirm that PERVs infect human cells, and we observe the horizont...
#1George M Church G MH-Index: 135
#2Luhan YangH-Index: 1
view all 3 authors...
L'invention concerne des procedes et des compositions de modification de l'ADN mitochondrial d'une cellule eucaryote a l'aide d'un ou plusieurs virus adeno-associes mitochondriaux specifiques pour administrer un ou plusieurs acides nucleiques codant pour un systeme CRISPR notamment une proteine Cas9 ou sa variante inactive par rapport a une nuclease et un ARN guide dans une mitochondrie pour l'expression a l'interieur des mitochondries. Le systeme Cas9 peut couper, casser ou reguler un acide nuc...
#1Gang WangH-Index: 39
#2Luhan YangH-Index: 15
Last.William T. PuH-Index: 57
view all 10 authors...
This protocol uses a doxycycline-inducible Cas9 transgene carried on a piggyBac transposon for robust, highly efficient and scarless genome editing of human iPSCs, enabling a parental line to be engineered to harbor many separate mutations.
#1Luhan YangH-Index: 15
#2Adrian W. BriggsH-Index: 18
Last.Emal LeshaH-Index: 4
view all 13 authors...
Precision genome engineering using homology donors and the endogenous DNA break repair machinery and recently CRISPR-Cas9 targeted APOBECs have been demonstrated. Here the authors design zinc-finger and TALE chimeric deaminases and investigate editing efficiency and off-target effects.
#1George M Church G MH-Index: 135
#2Luhan YangH-Index: 1
view all 3 authors...
La presente invention concerne un procede de modulation d'une partie ou de toutes les copies d'un gene dans une cellule incluant l'introduction, dans une cellule, d'une ou plusieurs sequences d'acides ribonucleiques (ARN) qui comprennent une partie qui est complementaire de l'ensemble ou d'une partie de la ou des multiples sequences d'acides nucleiques cibles, et une sequence d'acides nucleiques qui code pour une proteine Cas et le maintien des cellules dans des conditions dans lesquelles la pro...