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Hao Yin
Massachusetts Institute of Technology
Cas9Genome editingGeneticsBiologyCRISPR
56Publications
29H-index
4,625Citations
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Publications 42
Newest
#1Ying ZhangH-Index: 11
#2Hao YinH-Index: 29
Source
#1Haiwei Mou (UMMS: University of Massachusetts Medical School)H-Index: 15
#2Jordan L. Smith (UMMS: University of Massachusetts Medical School)H-Index: 6
Last. Wen Xue (UMMS: University of Massachusetts Medical School)H-Index: 32
view all 16 authors...
CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of β-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larg...
Source
#1Hao YinH-Index: 29
#2Chun-Qing SongH-Index: 15
Last. Dana Z. AndersonH-Index: 109
view all 14 authors...
Partial substitution of CRISPR RNAs with DNA nucleotides retains CRISPR–Cas9 genome editing activity while enhancing efficiency and specificity within cells, suggesting that DNA–RNA hybrids may be economical reagents for targeted genome editing.
45 CitationsSource
#1Per Hydbring (Harvard University)H-Index: 17
#2Yinan Wang (PKU: Peking University)H-Index: 2
Last. Piotr Sicinski (Harvard University)H-Index: 17
view all 7 authors...
ABSTRACTBy performing nine genome-wide microRNA (miRNA) screens, we recently uncovered a new class of miRNAs, which target multiple cyclins and cyclin-dependent kinases (CDKs). Systemic delivery of selected cell cycle-targeting miRNAs to mouse xenograft models resulted in potent anti-tumorigenic effects without affecting animals' health. Here, we provide an in-depth description of our miRNA screening methodology, analyses of selected cell cycle-targeting miRNAs, and discuss why miRNA therapy mig...
2 CitationsSource
#1Haiwei Mou (UMMS: University of Massachusetts Medical School)H-Index: 15
#2Jordan L. Smith (UMMS: University of Massachusetts Medical School)H-Index: 6
Last. Wen Xue (UMMS: University of Massachusetts Medical School)H-Index: 32
view all 17 authors...
CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of β-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larg...
49 CitationsSource
#1Roman L. BogoradH-Index: 24
#2Hao YinH-Index: 29
Last. Dana Z. AndersonH-Index: 109
view all 3 authors...
#1Hao YinH-Index: 29
#2Chun-Qing SongH-Index: 15
Last. Dana Z. AndersonH-Index: 109
view all 21 authors...
Efficient Cas9 genome editing in vivo is achieved without viral vectors using chemically modified single-guide RNAs.
88 CitationsSource
#1Hao YinH-Index: 29
#2Kevin J. KauffmanH-Index: 19
Last. Dana Z. AndersonH-Index: 109
view all 3 authors...
Genome editing has emerged as an attractive approach to therapeutically manipulate gene expression. Here, Anderson and colleagues provide an overview of genome-editing platforms, focusing on the methods and challenges of intracellular biomacromolecule delivery. Preclinical and clinical trials involving genome-editing technologies are also discussed.
119 CitationsSource
#1Chun-Qing Song (UMMS: University of Massachusetts Medical School)H-Index: 15
#2Yingxiang Li (Tongji University)H-Index: 9
Last. Wen Xue (UMMS: University of Massachusetts Medical School)H-Index: 32
view all 17 authors...
Background & Aims It has been a challenge to identify liver tumor suppressors or oncogenes due to the genetic heterogeneity of these tumors. We performed a genome-wide screen to identify suppressors of liver tumor formation in mice, using CRISPR-mediated genome editing. Methods We performed a genome-wide CRISPR/Cas9-based knockout screen of P53-null mouse embryonic liver progenitor cells that overexpressed MYC. We infected p53 −/− ; Myc ; Cas9 hepatocytes with the mGeCKOa lentiviral library of 6...
28 CitationsSource
#1Per Hydbring (KI: Karolinska Institutet)H-Index: 17
#2Yinan Wang (PKU: Peking University)H-Index: 2
Last. Piotr Sicinski (Harvard University)H-Index: 43
view all 27 authors...
Summary Cyclins and cyclin-dependent kinases (CDKs) are hyperactivated in numerous human tumors. To identify means of interfering with cyclins/CDKs, we performed nine genome-wide screens for human microRNAs (miRNAs) directly regulating cell-cycle proteins. We uncovered a distinct class of miRNAs that target nearly all cyclins/CDKs, which are very effective in inhibiting cancer cell proliferation. By profiling the response of over 120 human cancer cell lines, we derived an expression-based algori...
21 CitationsSource
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