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Chao Luan
Zhejiang University
9Publications
8H-index
122Citations
Publications 9
Newest
#1Deguang Song (ZJU: Zhejiang University)H-Index: 11
#2Xin Zong (ZJU: Zhejiang University)H-Index: 5
Last.Yizhen Wang (ZJU: Zhejiang University)H-Index: 24
view all 7 authors...
Abstract Intestinal barrier functions are altered during the development of sepsis. Cathelicidin antimicrobial peptides, such as LL-37 and mCRAMP, can protect animals against intestinal barrier dysfunction. Cathelicidin-BF (C-BF), a new cathelicidin peptide purified from the venom of the snake Bungarus fasciatus , has been shown to have both antimicrobial and anti-inflammatory properties. This study investigated whether C-BF pretreatment could protect the intestinal barrier against dysfunction i...
23 CitationsSource
#1Chao Luan (ZJU: Zhejiang University)H-Index: 8
#2Hai Wen Zhang (ZJU: Zhejiang University)H-Index: 2
Last.Yizhen Wang (ZJU: Zhejiang University)H-Index: 24
view all 6 authors...
Small ubiquitin-related modifier (SUMO) technology has been widely used in Escherichia coli expression systems to produce antimicrobial peptides. However, E. coli is a pathogenic bacterium that produces endotoxins and can secrete proteins into the periplasm, forming inclusion bodies. In our work, cathelicidin-BF (CBF), an antimicrobial peptide purified from Bungarus fasciatus venom, was produced in a Bacillus subtilis expression system using SUMO technology. The chimeric genes his-SUMO-CBF and h...
24 CitationsSource
#1Chao Luan (ZJU: Zhejiang University)H-Index: 8
#2Yong Gang Xie (ZJU: Zhejiang University)H-Index: 2
Last.Yizhen Wang (ZJU: Zhejiang University)H-Index: 24
view all 7 authors...
Antimicrobial peptides (AMPs) are part of the innate immune system of complex multicellular organisms. Despite the fact that AMPs show great potential as a novel class of antibiotics, the lack of a cost-effective means for their mass production limits both basic research and clinical use. In this work, we describe a novel expression system for the production of antimicrobial peptides in Escherichia coli by combining ΔI-CM mini-intein with the self-assembling amphipathic peptide 18A to drive the ...
8 CitationsSource
#1Feng Han (ZJU: Zhejiang University)H-Index: 21
#2Yanhua Gao (ZJU: Zhejiang University)H-Index: 4
Last.Yizhen Wang (ZJU: Zhejiang University)H-Index: 24
view all 6 authors...
Abstract Antibiotic treatment for microbial infections is under scrutiny due to increasing resistance to conventional antibiotics, warranting discovery of new classes of antibiotic agents. Antimicrobial peptides are part of the innate defense system found in nearly all organisms and possess bactericidal mechanisms that make it more difficult for bacteria to develop resistance. Porcine lactoferricin (LFP-20) is an antimicrobial peptide located in the N terminus of lactoferrin (LF). To develop nov...
16 CitationsSource
#1Yong-Gang XieH-Index: 5
#2Yifan LiuH-Index: 6
Last.Yizhen WangH-Index: 24
view all 8 authors...
Palustrin-OG1 (OG1) is a host defense peptide isolated from the frog Odorrana grahami. In this study, we analyzed the chemical properties, antimicrobial activities and cytotoxicities of OG1 and its derivatives to identify the most promising peptide as an antimicrobial agent. By increasing the net positive charge, amphipathicity and decreasing the mean hydrophobicity of OG1, the derivative named as OG2 exerted higher antimicrobial activity against bacteria but lower cytotoxicity against both porc...
2 CitationsSource
#1Yong-Gang Xie (ZJU: Zhejiang University)H-Index: 5
#2Feifei HanH-Index: 9
Last.Yizhen WangH-Index: 24
view all 8 authors...
OG2 is a modified antimicrobial peptide, that is, derived from the frog peptide Palustrin-OG1. It has high antimicrobial activity and low cytotoxicity, and it is therefore promising as a therapeutic agent. Both prokaryotic (Escherichia coli) and eukaryotic (Pichia pastoris) production host systems were used to produce OG2 in our previous study; however, it was difficult to achieve high expression yields and efficient purification. In this study, we achieved high-yield OG2 expression using the in...
9 CitationsSource
#1Yong-Gang XieH-Index: 5
#2Chao LuanH-Index: 8
Last.Yizhen WangH-Index: 24
view all 8 authors...
OG2 is a modified antimicrobial peptide of Palustrin-OG1 (OG1), which is derived from Odorrana grahami frog. OG2 has shown much higher selective antimicrobial activity and lower hemolytic activity than OG1, indicating OG2 may be a promising antimicrobial agent. In this study, we investigated three fusion partners, including thioredoxin, Mxe GyrA intein, and small ubiquitin-like modifier (SUMO), each fused with OG2, and examined their effects on the expression level and solubility of OG2 in Esche...
17 CitationsSource
#1Yifan Liu (ZJU: Zhejiang University)H-Index: 6
#2Chao Luan (ZJU: Zhejiang University)H-Index: 8
Last.Yizhen Wang (ZJU: Zhejiang University)H-Index: 24
view all 5 authors...
In the last few decades, long-term and high-dose usage of antibiotics in livestock diets has led to the emergence of antibiotic resistant bacteria, antibiotic residues in animal products and environmental pollution, adversely affecting animal health. Because of these concerns, a study screening cathelicidin peptides from different animal origins (i.e. protegrin-1 [PG-1], PMAP-23, LL-37, indolicidin and cathelicidin-BF [C-BF]) as antibiotic replacements with higher antimicrobial activity and lowe...
12 CitationsSource
#1Feifei Han (ZJU: Zhejiang University)H-Index: 9
#2Yifan Liu (ZJU: Zhejiang University)H-Index: 6
Last.Yizhen Wang (ZJU: Zhejiang University)H-Index: 24
view all 6 authors...
Animals posses a large variety of antimicrobial peptides (AMPs) that serve as effective components in innate host defenses against microbial infections. These antimicrobial peptides differ in amino acid composition, range of antimicrobial specificities, hemolysis, cytotoxicity and mechanisms of action. This study was designed to evaluate their therapeutic potential of the following six antimicrobial peptides initially found from animals: cecropin P1, indolicidin, LL-37, palustrin-OG1, LFP-20 and...
11 CitationsSource
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