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Takeshi Gotoh
Akita University
Molecular biologyEnzymeChemistryChromatographyBiochemistry
51Publications
13H-index
646Citations
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Publications 51
Newest
#1Takafumi Minato (Akita University)
Last. Saki Yokota (Akita University)H-Index: 1
view all 22 authors...
Angiotensin-converting enzyme 2 (ACE2) is critically involved in cardiovascular physiology and pathology, and is currently clinically evaluated to treat acute lung failure. Here we show that the B38-CAP, a carboxypeptidase derived from Paenibacillus sp. B38, is an ACE2-like enzyme to decrease angiotensin II levels in mice. In protein 3D structure analysis, B38-CAP homolog shares structural similarity to mammalian ACE2 with low sequence identity. In vitro, recombinant B38-CAP protein catalyzed th...
Source
#1Saki Yokota (Akita University)H-Index: 1
#2Takeshi Gotoh (Akita University)H-Index: 13
The common proteins rubber elongation factor (REF) and small rubber particle protein (SRPP) are associated with Hevea brasiliensis (Hb) rubber particles. They are involved in the stability of rubber particles and natural rubber biosynthesis. Recently, we cloned cDNAs encoding REF and SRPP from laticifers of Ficus carica (Fc). In the present study, we overexpressed REF/SRPPs (HbREF, FcREF, and FcSRPP) in Saccharomyces cerevisiae in anticipation of future rubber biosynthesis in recombinant yeast. ...
Source
#1William E. Bentley (UMD: University of Maryland, College Park)H-Index: 53
#2Donald K. MartinH-Index: 20
Last. Takeshi Gotoh (Akita University)H-Index: 13
view all 3 authors...
Source
#1Saki Yokota (Akita University)H-Index: 1
#2Yurina Suzuki (Akita University)H-Index: 1
Last. Takeshi Gotoh (Akita University)H-Index: 13
view all 6 authors...
Rubber elongation factor (REF) and small rubber particle protein (SRPP) are major latex proteins harvested from Hevea brasiliensis (the rubber tree; HbREF and HbSRPP, respectively). Their amino acid sequences exhibit high homology with each other. In the present study, we cloned two cDNAs encoding REF/SRPP-family proteins (FcREF/SRPP-1 and -2) from the laticifers of Ficus carica (fig tree). The amino acid sequences of these proteins showed high homology not only with each other but also with HbR...
1 CitationsSource
#1Takeshi Gotoh (Akita University)H-Index: 13
#2Ken-Ichi Kikuchi (Akita University)H-Index: 16
Last. Saori TakahashiH-Index: 22
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Source
#1Saori TakahashiH-Index: 22
#2Takeshi Gotoh (Akita University)H-Index: 13
Last. Kazuyuki HoriH-Index: 15
view all 8 authors...
Renin, a highly specific aspartic proteinase originated from kidney, is a ratelimiting enzyme in renin-angiotensin system. Recently, we developed effective expression system for recombinant human (rh) renin using baculovirus-insect cell expression system, and simple and rapid assay method for rh-renin using internally quenched fluorogenic (IQF) substrate. Using the purified rh-renin and the IQF substrate, we constructed rapid and sensitive renin inhibitor screening system. Using the system, we f...
2 CitationsSource
#1Tomoyo Nishihira (Kindai University)H-Index: 1
#2Asami Miyano (Kindai University)H-Index: 1
Last. Tamo Fukamizo (Kindai University)H-Index: 26
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1 CitationsSource
#1Masahiko Nagaki (Hirosaki University)H-Index: 7
#2Takumi Ichijo (Hirosaki University)H-Index: 1
Last. Hiroshi Sagami (Tohoku University)H-Index: 18
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Prenyltransferases catalyzes the basic isoprenoid chain elongation to produce prenyl diphosphates, which led to upward of 30,000 diverse isoprenoids as steroids, carotenoids, natural rubbers, and prenyl proteins. Here, we determined the reactivities of E- and Z-farnesyl diphosphate synthases (E- and Z-FPP synthases) isolated from Bacillus stearothermophilus and Thermobifida fusca, respectively. For this purpose we use the synthetic substrate analogs, 8-tetrahydropyran-2-yloxy-, 8-hydroxy- and 8-...
3 CitationsSource
#1Saori TakahashiH-Index: 22
#2Hiroki Ono (Akita University)H-Index: 2
Last. Toshihiro Sugiyama (Akita University)H-Index: 27
view all 5 authors...
Angiotensin I-converting enzyme (ACE, EC 3.4.15.1) is one of the most important enzymes in the renin-angiotensin system, a major blood pressure control system in mammals. We synthesized novel internally quenched fluorogenic (IQF) substrates for ACE based on the cleavage site of an angiotensin I, introducing N-methyl anthranic acid (Nma) and Ne-2,4-dinitrophenyl-lysine (Lys(Dnp))at the N- and C-terminal regions. Kinetic parameters of the synthesized IQF substrates Nma-Phe-His-Lys(Dnp) and Nma-His...
3 CitationsSource
#1Saori TakahashiH-Index: 22
#2Tetsuo TokiwanoH-Index: 14
Last. Takeshi GotohH-Index: 13
view all 6 authors...
We screened for human renin inhibitory activity in wild vegetables and found the activity in methanol extract of Aralia cordata. Physico-chemical data on the isolated inhibitors were identical to those of (–)-kaur-16-en-19-oic acid (kaurenic acid, KA) and pimaradienoic acids (PDA, 8:2 mixture of pimara-8(14), 15-dien-19-oic acid: pimara-9(11) 15-dien-19-oic acid). KA and PDA also inhibited porcine renin but had nearly no effect on other proteinases. This is the first demonstration of renin inhib...
3 Citations
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