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Puping Liang
Sun Yat-sen University
20Publications
8H-index
745Citations
Publications 20
Newest
#1Wei-Cheng Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Cheuk-Wa Wong (CUHK: The Chinese University of Hong Kong)H-Index: 6
Last.Jinfang Zhang (Guangzhou University of Chinese Medicine)H-Index: 17
view all 11 authors...
Background Circular RNAs are a class of regulatory RNA transcripts, which are ubiquitously expressed in eukaryotes. In the current study, we evaluate the function of a novel circRNA derived from the β-catenin gene locus, circβ-catenin.
14 CitationsSource
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Xiaowei Xie (SYSU: Sun Yat-sen University)H-Index: 7
Last.Zhou SongyangH-Index: 45
view all 12 authors...
The adenine base editor (ABE), capable of catalyzing A•T to G•C conversions, is an important gene editing toolbox. Here, we systematically evaluate genome-wide off-target deamination by ABEs using the EndoV-seq platform we developed. EndoV-seq utilizes Endonuclease V to nick the inosine-containing DNA strand of genomic DNA deaminated by ABE in vitro. The treated DNA is then whole-genome sequenced to identify off-target sites. Of the eight gRNAs we tested with ABE, 2–19 (with an average of 8.0) o...
10 CitationsSource
#1Yan Huang (SCAU: South China Agricultural University)
#2Chenhui Ding (SYSU: Sun Yat-sen University)H-Index: 14
Last.Junjiu Huang (SYSU: Sun Yat-sen University)H-Index: 19
view all 15 authors...
Source
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Junjiu Huang (SYSU: Sun Yat-sen University)H-Index: 19
1 CitationsSource
#1Zhuoheng Lin (SYSU: Sun Yat-sen University)H-Index: 3
#2Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
Last.Junjiu HuangH-Index: 19
view all 15 authors...
Abstract Mammalian spermatogenesis is maintained by a rare population of spermatogonial stem cells (SSCs), which are important for male fertility. SSCs remain a subset of undifferentiated spermatogonia, which can be isolated by a combination of surface markers. Specific markers to identify and isolate undifferentiated spermatogonia are lacking. Ussp1, a transcript previously annotated as long noncoding RNA (RIKEN cDNA 4933427D06, Gene ID: 232217), virtually encodes a membrane protein, USSP1, in ...
Source
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Hongwei Sun (SYSU: Sun Yat-sen University)H-Index: 5
Last.Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 45
view all 13 authors...
4 CitationsSource
#1Shouheng Jin (SYSU: Sun Yat-sen University)H-Index: 5
#2Xiya Zhang (SYSU: Sun Yat-sen University)H-Index: 8
Last.Jun Cui (SYSU: Sun Yat-sen University)H-Index: 18
view all 11 authors...
4 CitationsSource
#1Junyan Feng (SYSU: Sun Yat-sen University)H-Index: 1
#2Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
Last.Junjiu Huang (Guangzhou Medical University)H-Index: 19
view all 8 authors...
Source
#1Yuxi Chen (SYSU: Sun Yat-sen University)H-Index: 6
#2Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
Last.Junjiu HuangH-Index: 19
view all 16 authors...
Spermatids undergo the final steps of maturation during spermiogenesis, a process that necessitates extensive rearrangement of organelles such as the mitochondria. Male infertility has been linked to mitochondrial disorder, for example, hypospermatogenesis and asthenozoospermia. However, the mechanisms that regulate mitochondrial dynamics during spermiogenesis remain largely unknown. We found the glycerol kinase (Gyk)-like proteins glycerol kinase-like 1 (Gykl1) and glycerol kinase 2 (Gk2) were ...
3 CitationsSource
#1Yuanyan Xiong (SYSU: Sun Yat-sen University)H-Index: 7
#2Xiaowei Xie (SYSU: Sun Yat-sen University)H-Index: 7
Last.Zhiming Dai (SYSU: Sun Yat-sen University)H-Index: 6
view all 7 authors...
The CRISPR/Cas System has been shown to be an efficient and accurate genome-editing technique. There exist a number of tools to design the guide RNA sequences and predict potential off-target sites. However, most of the existing computational tools on gRNA design are restricted to small deletions. To address this issue, we present pgRNAFinder, with an easy-to-use web interface, which enables researchers to design single or distance-free paired-gRNA sequences. The web interface of pgRNAFinder con...
1 CitationsSource
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