#1Yuxi Chen(SYSU: Sun Yat-sen University)H-Index: 6
#2Shengyao Zhi(SYSU: Sun Yat-sen University)H-Index: 3
Last. Yizhi Liu(SYSU: Sun Yat-sen University)H-Index: 1
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Abstract Herpes simplex keratitis (HSK) is the most common cause of corneal blindness in developed nations, caused by primary or recurrent herpes simplex virus 1 (HSV-1) infection of the cornea. Latent infection of HSV-1, especially in the trigeminal ganglion (TG), causes recurrence of HSV-1 infection. As antiviral treatment is not effective on latent HSV-1, to test the possibility of inhibiting HSV-1 by SpCas9 or SaCas9, ICP0 and ICP4, two important genes required for HSV-1 replication and reac...
Class 2 CRISPR–Cas proteins have been widely developed as genome editing and transcriptional regulating tools. Class 1 type I CRISPR–Cas constitutes ~60% of all the CRISPR–Cas systems. However, only type I–B and I–E systems have been used to control mammalian gene expression and for genome editing. Here we demonstrate the feasibility of using type I–F system to regulate human gene expression. By fusing transcription activation domain to Pseudomonas aeruginosa type I–F Cas proteins, we activate g...
Cytosine and adenine base editors are promising new tools for introducing precise genetic modifications that are required to generate disease models and to improve traits in pigs. Base editors can catalyze the conversion of C→T (C>T) or A→G (A>G) in the target site through a single guide RNA. Injection of base editors into the zygote cytoplasm can result in the production of offspring with precise point mutations, but most F0 are mosaic, and breeding of F1 heterozygous pigs is time-intensive. He...
#1Meixin Chen(SYSU: Sun Yat-sen University)H-Index: 1
#2Zhiyao Zhao(SYSU: Sun Yat-sen University)H-Index: 2
Last. Jun Cui(SYSU: Sun Yat-sen University)H-Index: 20
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The noncanonical NF‐κB signaling pathway plays a critical role in a variety of biological functions including chronic inflammation and tumorigenesis. Activation of noncanonical NF‐κB signaling largely relies on the abundance as well as the processing of the NF‐κB family member p100/p52. Here, TRIM14 is identified as a novel positive regulator of the noncanonical NF‐κB signaling pathway. TRIM14 promotes noncanonical NF‐κB activation by targeting p100/p52 in vitro and in vivo. Furthermore, a mecha...
Last. Junjiu Huang(Guangzhou Medical University)H-Index: 22
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Abstract Base editors (BEs) is a powerful tool that could edit the genome efficiently without inducing DNA double-strand breaks (DSBs). Recently, off-target DNA and RNA mutations of BEs were found, rasing concerns about its precision and safety. Here we discuss some strategies that might eliminate off-target effects of BEs.
#1Xiaohu Su(SYSU: Sun Yat-sen University)H-Index: 1
#2Wei Chen(SYSU: Sun Yat-sen University)H-Index: 1
Last. Junjiu Huang(Guangzhou Medical University)H-Index: 22
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Abstract: Genetically modified pigs represent a great promise for generating models of human diseases and producing new breeds. Generation of genetically edited pigs using somatic cell nuclear transfer (SCNT) or zygote cytoplasmic microinjection is a tedious process due to the low developmental rate or mosaicism of the founder (F0). Herein, we developed a method termed germinal vesicle oocyte gene editing (GVGE) to produce non-mosaic porcine embryos by editing maternal alleles during the GV to M...