Jiankui Zhou
ShanghaiTech University
Publications 5
#1Yiqiang Cui (Nanjing Medical University)H-Index: 11
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last.Weizhi Ji (Kunming University of Science and Technology)H-Index: 25
view all 15 authors...
Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination
13 CitationsSource
#1Yu Kang (Kunming University of Science and Technology)H-Index: 8
#2Bo Zheng (Nanjing Medical University)H-Index: 9
Last.Xingxu Huang (ShanghaiTech University)H-Index: 19
view all 16 authors...
Mutations in the DAX1 locus cause X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism (HH), which manifest with primary adrenal insufficiency and incomplete or absent sexual maturation, respectively. The associated defects in spermatogenesis can range from spermatogenic arrest to Sertoli cell only syndrome. Conclusions from Dax1 knockout mouse models provide only limited insight into AHC/HH disease mechanisms, because mouse models exhibit more extensive abnormalities in...
40 CitationsSource
#1Jiankui Zhou (National Resource Center)H-Index: 5
#2Jianying Wang (National Resource Center)H-Index: 10
Last.Xingxu Huang (National Resource Center)H-Index: 19
view all 9 authors...
The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system is a versatile RNA-guided mammalian genome modification system. One-step generation of mouse genome targeting has been achieved by co-microinjection of one-cell stage embryos with Cas9 mRNA and small/single guide (sg)RNA. Many studies have focused on enhancing the efficiency of this system. In the present study, we report that simultaneous use of dual sgRNAs to target an individual ...
74 CitationsSource
#1Yuyu NiuH-Index: 17
#2Bin Shen (National Resource Center)H-Index: 18
Last.Jiahao Sha (Nanjing Medical University)H-Index: 34
view all 28 authors...
Summary Monkeys serve as important model species for studying human diseases and developing therapeutic strategies, yet the application of monkeys in biomedical researches has been significantly hindered by the difficulties in producing animals genetically modified at the desired target sites. Here, we first applied the CRISPR/Cas9 system, a versatile tool for editing the genes of different organisms, to target monkey genomes. By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos, w...
628 CitationsSource
#1Jiankui Zhou (National Resource Center)H-Index: 5
#2Bin Shen (National Resource Center)H-Index: 18
Last.Xingxu Huang (National Resource Center)H-Index: 19
view all 12 authors...
Taking advantage of the multiplexable genome engineering feature of the CRISPR/Cas9 system, we sought to generate different kinds of immunodeficient mouse strains by embryo co-microinjection of Cas9 mRNA and multiple sgRNAs targeting mouse B2m, Il2rg, Prf1, Prkdc, and Rag1. We successfully achieved multiple gene modifications, fragment deletion, double knockout of genes localizing on the same chromosome, and got different kinds of immunodeficient mouse models with different heritable genetic mod...
81 CitationsSource