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Weidong Cui
University of Washington
26Publications
15H-index
772Citations
Publications 26
Newest
#1Hanliu Wang (WashU: Washington University in St. Louis)H-Index: 4
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 7 authors...
Apolipoprotein E (apoE) is an essential protein in lipid and cholesterol metabolism. Although the three common isoforms in humans differ only at two sites, their consequences in Alzheimer’s disease (AD) are dramatically different: only the e4 allele is a major genetic risk factor for late-onset Alzheimer’s disease. The isoforms exist as a mixture of oligomers, primarily tetramer, at low μM concentrations in a lipid-free environment. This self-association is involved in equilibrium with the lipid...
Source
#1Guomin ShenH-Index: 3
#2Shuang LiH-Index: 3
Last.Weikai LiH-Index: 14
view all 7 authors...
Mass spectrometry-based footprinting is an emerging approach for studying protein structure. Because integral membrane proteins are difficult targets for conventional structural biology, we recently developed a mass spectrometry (MS) footprinting method to probe membrane protein–drug interactions in live cells. This method can detect structural differences between apo and drug-bound states of membrane proteins, with the changes inferred from MS quantification of the cysteine modification pattern...
3 CitationsSource
#1Ming Cheng (WashU: Washington University in St. Louis)H-Index: 6
#2Bojie Zhang (WashU: Washington University in St. Louis)H-Index: 4
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 4 authors...
Described is a novel, laser-initiated radical trifluoromethylation for protein footprinting and its broad residue coverage. .CF3 reacts with 18 of the 20 common amino acids, including Gly, Ala, Ser, Thr, Asp, and Glu, which are relatively silent with regard to .OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to myoglobin show that the approach is...
11 CitationsSource
#1Ben Niu (WashU: Washington University in St. Louis)H-Index: 2
#2Brian C. Mackness (UMMS: University of Massachusetts Medical School)H-Index: 2
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 8 authors...
Incorporation of a reporter peptide in solutions submitted to fast photochemical oxidation of proteins (FPOP) allows for the correction of adventitious scavengers and enables the normalization and comparison of time-dependent results. Reporters will also be useful in differential experiments to control for the inclusion of a radical-reactive species. This incorporation provides a simple and quick check of radical dosage and allows comparison of FPOP results from day-to-day and lab-to-lab. Use of...
9 CitationsSource
#1Jamie R. Wallen (WCU: Western Carolina University)H-Index: 1
#2Hao F. Zhang (WashU: Washington University in St. Louis)H-Index: 44
Last.Tom Ellenberger (WashU: Washington University in St. Louis)H-Index: 17
view all 10 authors...
Summary The physical organization of DNA enzymes at a replication fork enables efficient copying of two antiparallel DNA strands, yet dynamic protein interactions within the replication complex complicate replisome structural studies. We employed a combination of crystallographic, native mass spectrometry and small-angle X-ray scattering experiments to capture alternative structures of a model replication system encoded by bacteriophage T7. Two molecules of DNA polymerase bind the ring-shaped pr...
8 CitationsSource
#1Guomin ShenH-Index: 3
#2Weidong CuiH-Index: 15
Last.Weikai LiH-Index: 14
view all 12 authors...
Mass spectrometry and biochemical analyses reveal that the major form of VKOR found in cells features a disulfide bond between Cys51 and Cys132, and this intermediate is the target of the anticoagulant drug warfarin.
16 CitationsSource
#1Y. Zhang (Pfizer)H-Index: 11
#2Weidong Cui (WashU: Washington University in St. Louis)H-Index: 15
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 7 authors...
Native mass spectrometry (MS) and top-down electron-capture dissociation (ECD) combine as a powerful approach for characterizing large proteins and protein assemblies. Here, we report their use to study an antibody Fab (Fab-1)–VEGF complex in its near-native state. Native ESI with analysis by FTICR mass spectrometry confirms that VEGF is a dimer in solution and that its complex with Fab-1 has a binding stoichiometry of 2:2. Applying combinations of collisionally activated dissociation (CAD), ECD...
9 CitationsSource
#1Yue LuH-Index: 6
#2Hao F. ZhangH-Index: 44
Last.Robert E. BlankenshipH-Index: 63
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We report a top-down proteomic analysis of the membrane-bound peripheral light-harvesting complex LH2 isolated from the purple photosynthetic bacterium Rhodobacter sphaeroides. The LH2 complex is coded for by the puc operon. The Rb. sphaeroides genome contains two puc operons, designated puc1BAC and puc2BA. Although previous work has shown consistently that the LH2 β polypeptide coded by the puc2B gene was assembled into LH2 complexes, there are contradictory reports as to whether the Puc2A poly...
5 CitationsSource
#1Weidong CuiH-Index: 15
#2Hao F. ZhangH-Index: 44
Last.Michael L. GrossH-Index: 75
view all 4 authors...
Native spray has the potential to probe biophysical properties of protein assemblies. Here we report an investigation using both ECD top-down sequencing with an FTICR mass spectrometer and ion mobility (IM) measurements on a Q-TOF to investigate the collisionally induced unfolding of a native-like heterogeneous tetrameric assembly, human hemoglobin (hHb), in the gas phase. To our knowledge, this is the first report combining ECD and ion-mobility data on the same target protein assembly to deline...
15 CitationsSource
Mass spectrometric de-novo sequencing was applied to review the amino acid sequence of a commercially available recombinant protein G´ with great scientific and economic importance. Substantial deviations to the published amino acid sequence (Uniprot Q54181) were found by the presence of 46 additional amino acids at the N-terminus, including a so-called “His-tag” as well as an N-terminal partial α-N-gluconoylation and α-N-phosphogluconoylation, respectively. The unexpected amino acid sequence of...
8 CitationsSource
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