Shilpa Jamwal
Translational Health Science and Technology Institute
Publications 8
#1Manu KandpalH-Index: 1
#2Suruchi AggarwalH-Index: 4
Last.Amit Kumar YadavH-Index: 11
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Microbes portray an immense capacity to colonize their niche by sequestering all resources for themselves and producing antibiotics to thwart the growth of other microbes. Antibiotic resistance by microbes evolved to overcome this challenge. Humans have long exploited the antibiotics to control infectious diseases. This development greatly improved the global health and decreased the mortality rate, thereby increasing average life expectancy. Drug resistance also arose like natural antibiotic re...
1 CitationsSource
#1Ajay Kumar (International Centre for Genetic Engineering and Biotechnology)H-Index: 9
#2Shilpa Jamwal (Translational Health Science and Technology Institute)H-Index: 6
Last.Kanury V. S. Rao (International Centre for Genetic Engineering and Biotechnology)H-Index: 24
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Here we provide data for SILAC and iTRAQ based hyperplexing combined with BONCAT based click chemistry for selective enrichment of newly synthesized proteins secreted by THP1 macrophages at various time points after infection with four different strains of Mycobacterium tuberculosis. The macrophages were infected with H37Ra, H37Rv, BND433 and JAL2287 strains of M. tuberculosis. Newly-synthesized secreted host proteins were observed, starting from six hours post-infection till 26 h, at 4 h interv...
3 CitationsSource
#1Shilpa JamwalH-Index: 6
#2Parul MehrotraH-Index: 2
Last.Kanury V. S. RaoH-Index: 24
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Survival of Mycobacterium tuberculosis (Mtb) within the host macrophage is mediated through pathogen-dependent inhibition of phagosome-lysosome fusion, which enables bacteria to persist within the immature phagosomal compartment. By employing ultrastructural examination of different field isolates supported by biochemical analysis, we found that some of the Mtb strains were in fact poorly adapted for subsistence within endocytic vesicles of infected macrophages. Instead, through a mechanism invo...
34 CitationsSource
Abstract Mycobacterium tuberculosis is an adaptable intracellular pathogen, existing in both dormant as well as active disease-causing states. Here, we report systematic proteomic analyses of four strains, H37Ra, H37Rv, and clinical isolates BND and JAL, to determine the differences in protein expression patterns that contribute to their virulence and drug resistance. Resolution of lysates of the four strains by liquid chromatography, coupled to mass spectrometry analysis, identified a total of ...
17 CitationsSource
#1Najmuddin Saquib (Jaipur National University)H-Index: 2
#2Shilpa Jamwal (Translational Health Science and Technology Institute)H-Index: 6
Last.Venkatasamy Manivel (International Centre for Genetic Engineering and Biotechnology)H-Index: 12
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Even though endoplasmic reticulum (ER) stress associated with mycobacterial infection has been well studied, the molecular basis of ER as a crucial organelle to determine the fate of Mtb is yet to be established. Here, we have studied the ability of Mtb to manipulate the ultrastructural architecture of macrophage ER and found that the ER-phenotypes associated with virulent (H37Rv) and avirulent (H37Ra) strains were different: a rough ER (RER) with the former against a smooth ER (SER) with the la...
15 CitationsSource
#1Parul MehrotraH-Index: 2
#2Shilpa JamwalH-Index: 6
Last.Kanury V. S. RaoH-Index: 24
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The success of Mycobacterium tuberculosis as a pathogen derives from its facile adaptation to the intracellular milieu of human macrophages. To explore this process, we asked whether adaptation also required interference with the metabolic machinery of the host cell. Temporal profiling of the metabolic flux, in cells infected with differently virulent mycobacterial strains, confirmed that this was indeed the case. Subsequent analysis identified the core subset of host reactions that were targete...
45 CitationsSource
#1Shilpa JamwalH-Index: 6
#2Mukul Kumar MidhaH-Index: 4
Last.Venkatasamy ManivelH-Index: 12
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To probe how the pathogen Mycobacterium tuberculosis controls host cellular death pathways, we compared mitochondrial responses in human macrophages infected either with the avirulent mycobacterial strain H37Ra, or its virulent counterpart H37Rv. Following H37Ra infection, induction of the apoptotic response was foreshadowed by the early suppression of stress-induced mitochondrial activity. In contrast, mitochondria in H37Rv-infected cells displayed robust activity with increased membrane potent...
34 CitationsSource
#1Varshneya Singh (International Centre for Genetic Engineering and Biotechnology)H-Index: 1
#2Shilpa Jamwal (International Centre for Genetic Engineering and Biotechnology)H-Index: 6
Last.Kanury V. S. Rao (International Centre for Genetic Engineering and Biotechnology)H-Index: 24
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Summary Upon infection, Mycobacterium tuberculosis (Mtb) metabolically alters the macrophage to create a niche that is ideally suited to its persistent lifestyle. Infected macrophages acquire a "foamy" phenotype characterized by the accumulation of lipid bodies (LBs), which serve as both a source of nutrients and a secure niche for the bacterium. While the functional significance of the foamy phenotype is appreciated, the biochemical pathways mediating this process are understudied. We found tha...
131 CitationsSource