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Malte Drescher
University of Konstanz
Site-directed spin labelingChemistryElectron paramagnetic resonanceNuclear magnetic resonanceSpin-½
118Publications
23H-index
1,489Citations
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Publications 123
Newest
#1Philipp RohseH-Index: 1
#2Sabrina WeickertH-Index: 2
Last. Valentin WittmannH-Index: 27
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Multivalent ligand-protein interactions are a key concept in biology mediating, for example, signalling and adhesion. Multivalent ligands often have tremendously increased binding affinities. However, they also can cause crosslinking of receptor molecules leading to precipitation of ligand-receptor complexes. Plaque formation due to precipitation is a known characteristic of numerous fatal diseases limiting a potential medical application of multivalent ligands with a precipitating binding mode....
1 CitationsSource
#2Pia WidderH-Index: 1
Last. Malte Drescher (University of Konstanz)H-Index: 23
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Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a powerful tool in protein structural research. Nitroxides are highly suitable spin labeling reagents, but suffer from limited stability, particularly in the cellular environment. Herein we present the synthesis of a maleimide- and an azide-modified tetraethyl-shielded isoindoline-based nitroxide (M- and Az-TEIO) for labeling of cysteines or the noncanonical amino acid para-ethynyl-l-phen...
1 CitationsSource
#1Sabrina Weickert (University of Konstanz)H-Index: 2
#2Magdalena Wawrzyniuk (UU: Utrecht University)
Last. Malte Drescher (University of Konstanz)H-Index: 23
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Aggregation of the microtubule-associated protein Tau is a hallmark of Alzheimer’s disease with Tau oligomers suspected as the most toxic agent. Tau is a client of the molecular chaperone Hsp90, although it is unclear whether and how the chaperone massages the structure of intrinsically disordered Tau. Using electron paramagnetic resonance, we extract structural information from the very broad conformational ensemble of Tau: Tau in solution is highly dynamic and polymorphic, although “paper clip...
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#1Andreas Scherer (University of Konstanz)
#2Sonja Tischlik (University of Konstanz)
Last. Malte Drescher (University of Konstanz)H-Index: 23
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Abstract. EPR distance determination in the nanometre region has become an important tool for studying the structure and interaction of macromolecules. Arbitrary waveform generators (AWGs), which have recently become commercially available for EPR spectrometers, have the potential to increase the sensitivity of the most common technique double electron-electron resonance (DEER, also called PELDOR), as they allow the generation of broadband pulses. There are several families of broadband pulses, ...
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#1Pia WidderH-Index: 1
#2Julian SchuckH-Index: 1
Last. Malte DrescherH-Index: 23
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Structural studies on proteins directly in their native environment are required for a comprehensive understanding of their function. Electron paramagnetic resonance (EPR) spectroscopy and in particular double electron-electron resonance (DEER) distance determination are suited to investigate spin-labeled proteins directly in the cell. The combination of intracellular bioorthogonal labeling with in-cell DEER measurements does not require additional purification or delivery steps of spin-labeled ...
1 CitationsSource
#1Dennis Bücker (University of Konstanz)H-Index: 2
#2Annika Sickinger (University of Konstanz)H-Index: 1
Last. Malte Drescher (University of Konstanz)H-Index: 23
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Synthetic polymers are mixtures of chains with different lengths, and their chain length and chain conformation is often experimentally characterized by ensemble averages. We demonstrate that Double-Electron-Electron-Resonance (DEER) spectroscopy can reveal the chain length distribution, and chain conformation and flexibility of the individual n-mers in oligo-(9,9-dioctylfluorene) from controlled Suzuki-Miyaura Coupling Polymerization (cSMCP). The required spin-labeled chain ends were introduced...
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#2Mykhailo AzarkhH-Index: 9
Last. Rainer F. WinterH-Index: 30
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We report on 18-valence-electron ferrocenyl vinylruthenium conjugates 4-Fc-C6H4-CH═CH-Ru(CO)Cl(PiPr3)2(PhR2acac) where PhR2acac denotes a diphenylacetylacetonato ligand with two 4-substituted (R = OMe, 2-OMe; R = H, 2-H; R = F, 2-F; R = CF3, 2-CF3) or perdeuterated (R = C6D5, 2-D) phenyl substituents. The complexes undergo two reversible one-electron oxidations whose potentials depend on the aryl substituents R. The one-electron oxidized forms of these complexes exist as two different isomers, w...
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#1Anna BieberH-Index: 2
#2Malte DrescherH-Index: 23
Electron paramagnetic resonance (EPR) experiments on spin-labeled biomacromolecules yield information on the local conformation and dynamics of the labeling site and precise long-range distance constraints between pairs of spin labels. The technique is independent of the size of the studied macromolecule and essentially background-free in most environments. Consequently, EPR spectroscopy is well suited for the structural characterization of biomacromolecules directly in the cell, with double ele...
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#1Sabrina Weickert (University of Konstanz)H-Index: 2
#2Magdalena Wawrzyniuk (UU: Utrecht University)
Last. Malte Drescher (University of Konstanz)H-Index: 23
view all 5 authors...
Aggregation of the microtubule-associated protein Tau is a hallmark of Alzheimers disease with Tau oligomers suspected as the most toxic agent. Tau is a client of Hsp90, though it is unclear whether and how the chaperone massages the structure of intrinsically disordered Tau. Using electron paramagnetic resonance, we extract structural information from the very broad conformational ensemble of Tau: Tau in solution is highly dynamic and polymorphic, though paper-clip-shaped by long-range contacts...
Source
#1Sebastian HöfelH-Index: 2
#2Michael K. FixH-Index: 12
Last. Malte DrescherH-Index: 23
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