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Keiji Nishida
Kobe University
61Publications
23H-index
2,513Citations
Publications 61
Newest
#1Yuichi Furuhata (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 3
#2Ayako Sakai (AIST: National Institute of Advanced Industrial Science and Technology)
Last.Yoshio Kato (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 23
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Genome engineering in plants is highly dependent on the availability of effective molecular techniques. Despite vast quantities of research, genome engineering in plants is still limited in terms of gene delivery, which requires the use of infectious bacteria or harsh conditions owing to the difficulty delivering biomaterial into plant cells through the cell wall. Here, we describe a method that uses electroporation-mediated protein delivery into cultured Arabidopsis thaliana cells possessing an...
#1Kenta Katayama (Kobe University)
#2Hitoshi Mitsunobu (Kobe University)H-Index: 9
Last.Keiji Nishida (Kobe University)H-Index: 23
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Technologies harnessing CRISPR systems have been rapidly evolving and expanding the capacity of researchers for understanding of mammalian cell behavior and its underlying mechanisms through genome and epigenome manipulations. In this review, we summarized the recent developments of CRISPR-based technologies for genetic and epigenetic modifications that include engineering of Cas9 for PAM simplification, non-cleaving base editing tools and alteration of gene expression. Applications such as geno...
#1Rina C. Sakata (UTokyo: University of Tokyo)
#2Soh Ishiguro (UTokyo: University of Tokyo)H-Index: 2
Last.Osamu Nureki (UTokyo: University of Tokyo)H-Index: 53
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While several Cas9-derived base editors have been developed to induce either C-to-T or A-to-G mutation at target genomic sites, the possible genome editing space when using the current base editors remains limited. Here, we present a novel base editor, Target-ACE, which integrates the abilities of both of the previously developed C-to-T and A-to-G base editors by fusing an activation-induced cytidine deaminase (AID) and an engineered tRNA adenosine deaminase (TadA) to a catalytically impaired St...
#1Zenpei Shimatani (Kobe University)H-Index: 9
#2Tohru Ariizumi (University of Tsukuba)H-Index: 14
Last.Keiji Nishida (Kobe University)H-Index: 23
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#1Shingo Tanaka (Kyoto University)H-Index: 3
#2Shin Yoshioka (Kobe University)H-Index: 1
Last.Shingo Maegawa (Kyoto University)H-Index: 12
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To date, several genome editing technologies have been developed and are widely utilized in many fields of biology. Most of these technologies, if not all, use nucleases to create DNA double-strand breaks (DSBs), raising the potential risk of cell death and/or oncogenic transformation. The risks hinder their therapeutic applications in humans. Here, we show that in vivo targeted single-nucleotide editing in zebrafish, a vertebrate model organism, can be successfully accomplished with the Target-...
#1Yumiko Nambu-Nishida (Kobe University)H-Index: 2
#2Keiji Nishida (Kobe University)H-Index: 23
Last.Akihiko Kondo (Kobe University)H-Index: 68
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Kluyveromyces marxianus is a thermotolerant, crabtree-negative yeast, which preferentially directs metabolism (e.g., from the tricarboxylic acid cycle) to aerobic alcoholic fermentation. Thus K. marxianus has great potential for engineering to produce various materials under aerobic cultivation conditions. In this study, we engineered K. marxianus to produce and secrete a single-chain antibody (scFv), a product that is highly valuable but has historically proven difficult to generate at large sc...
#1Zenpei Shimatani (Kobe University)H-Index: 9
#2Ushio Fujikura (Kobe University)H-Index: 7
Last.Akihiko Kondo (Kobe University)H-Index: 68
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Abstract The CRISPR/Cas9 system is a revolutionary genome-editing tool for directed gene editing in various organisms. Cas9 variants can be applied as molecular homing devices when combined with various functional effectors such as transcriptional activators or DNA modification enzymes. Target-AID is a synthetic complex of nuclease deficient Cas9 fused to an activation-induced cytidine deaminase (AID) that enables targeted nucleotide substitution (C to T or G to A). We previously demonstrated th...
#1Zenpei Shimatani (Kobe University)H-Index: 9
#2Ushio Fujikura (Kobe University)H-Index: 7
Last.Akihiko Kondo (Kobe University)H-Index: 68
view all 6 authors...
Abstract Acetolactate synthase (ALS) catalyzes the initial step in the biosynthesis of branched-chain amino acids, and is highly conserved from bacteria to higher plants. ALS is encoded by a single copy gene in rice genome and is a target enzyme of several classes of herbicides. Although ALS mutations conferring herbicide-resistance property to plants are well documented, effect of Imazamox (IMZ) on rice and the mutations in ALS correlated with IMZ tolerance were unclear. In this article, the ef...
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