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Martin Caffrey
Trinity College, Dublin
255Publications
54H-index
12.1kCitations
Publications 255
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#1Meriem El Ghachi (University of Liège)H-Index: 6
#2Nicole Howe (Trinity College, Dublin)H-Index: 8
Last.Fred Kerff (University of Liège)H-Index: 1
view all 11 authors...
As a protective envelope surrounding the bacterial cell, the peptidoglycan sacculus is a site of vulnerability and an antibiotic target. Peptidoglycan components, assembled in the cytoplasm, are shuttled across the membrane in a cycle that uses undecaprenyl-phosphate. A product of peptidoglycan synthesis, undecaprenyl-pyrophosphate, is converted to undecaprenyl-phosphate for reuse in the cycle by the membrane integral pyrophosphatase, BacA. To understand how BacA functions, we determine its crys...
#1Wanchao YinH-Index: 1
#2X. Edward Zhou (CAS: Chinese Academy of Sciences)H-Index: 22
Last.Xiaoxi Wang (CAS: Chinese Academy of Sciences)H-Index: 2
view all 21 authors...
5-hydroxytryptamine (5-HT, also known as serotonin) regulates many physiological processes through the 5-HT receptor family. Here we report the crystal structure of 5-HT1B subtype receptor (5-HT1BR) bound to the psychotropic serotonin receptor inverse agonist methiothepin (MT). Crystallization was facilitated by replacing ICL3 with a novel optimized variant of BRIL (OB1) that enhances the formation of intermolecular polar interactions, making OB1 a potential useful tool for structural studies of...
#1Coilín Boland (Trinity College, Dublin)H-Index: 6
#2Samir Olatunji (Trinity College, Dublin)
Last.Martin Caffrey (Trinity College, Dublin)H-Index: 54
view all 10 authors...
Label-free differential scanning fluorimetry (DSF) is a relatively new method for evaluating the stability of proteins. It can be used as a screening tool for downstream applications such as crystallization. The method is attractive in that it requires miniscule quantities of proteins, it can be performed using intrinsic tryptophan and tyrosine fluorescence, and, with the right equipment, it is easy to perform. To date, the method has been used with proteins in liquid solutions and dispersions. ...
#1Chia-Ying HuangH-Index: 5
#2Vincent OliericH-Index: 14
Last.Martin CaffreyH-Index: 54
view all 11 authors...
#1Chia-Ying HuangH-Index: 5
#2Vincent OliericH-Index: 14
Last.Martin CaffreyH-Index: 54
view all 11 authors...
#1Chia-Ying HuangH-Index: 5
#2Vincent OliericH-Index: 14
Last.Martin CaffreyH-Index: 54
view all 11 authors...
#1Chia-Ying Huang (Paul Scherrer Institute)H-Index: 5
#2Vincent Olieric (Paul Scherrer Institute)H-Index: 14
Last.Kay DiederichsH-Index: 51
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De novo membrane protein structure determination is often limited by the availability of large crystals and the difficulties in obtaining accurate diffraction data for experimental phasing. Here we present a method that combines in situ serial crystallography with de novo phasing for fast, efficient membrane protein structure determination. The method enables systematic diffraction screening and rapid data collection from hundreds of microcrystals in in meso crystallization wells without the nee...
#1Chia-Ying HuangH-Index: 5
#2Vincent OliericH-Index: 14
Last.Martin CaffreyH-Index: 54
view all 11 authors...
#1Isabelle MartielH-Index: 8
#2Vincent OliericH-Index: 6
Last.Meitian WangH-Index: 25
view all 4 authors...
In situ data collection for macromolecular crystallography has undergone rapid development in the last decade. From routine crystal hit screening in X-ray-friendly SBS-format plates to serial data collection in dedicated setups, its field of application has expanded significantly. The in situ approach has benefitted from progress in beamline instrumentation and automation and from new modes of sample delivery, inspired by serial crystallography using X-ray free-electron lasers. In this review, t...
#1M. Wiktor (Trinity College, Dublin)
#2Martin Caffrey (Trinity College, Dublin)H-Index: 54
Abstract Lipoproteins are important for bacterial growth and virulence and interest in them as targets for antibiotic development is growing. Lipoprotein N -acyl transferase (Lnt) catalyzes the final step in the lipoprotein posttranslational processing pathway. The mature lipoprotein can remain in the inner membrane or be trafficked to the outer membrane in the case of diderm prokaryotes. With a view to obtaining high-resolution crystal structures of membrane integral Lnt for use in drug discove...
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