Match!
Mitch McVey
Tufts University
38Publications
17H-index
1,842Citations
Publications 38
Newest
Abstract Werner syndrome (WS) is an autosomal recessive progeroid disease characterized by patients' early onset of aging, increased risk of cancer and other age-related pathologies. WS is caused by mutations in WRN, a RecQ helicase that has essential roles responding to DNA damage and preventing genomic instability. While human WRN has both an exonuclease and helicase domain, Drosophila WRNexo has high genetic and functional homology to only the exonuclease domain of WRN. Like WRN-deficient hum...
#1Brenna M.G. Gormally (Tufts University)H-Index: 1
#2Rory G. Fuller (Tufts University)H-Index: 1
Last.L. Michael Romero (Tufts University)H-Index: 48
view all 4 authors...
Abstract Corticosterone does not change in consistent ways across species and contexts, making it challenging to use as an indicator of chronic stress. We assessed DNA damage as a potential metric that could be a more integrative stress measurement with direct links to health. We captured free-living house sparrows, took an immediate blood sample, and transferred them to the laboratory, exposing them to the chronic stress of captivity. Biweekly blood and weight samples were then taken for 4 week...
#1Terrence Hanscom (Tufts University)H-Index: 1
#2Varandt Y. Khodaverdian (Tufts University)H-Index: 2
Last.Mitch McVey (Tufts University)H-Index: 17
view all 3 authors...
Abstract In this chapter, we describe a method for the recovery and analysis of alternative end-joining (alt-EJ) DNA double-strand break repair junctions following I- Sce I cutting in Drosophila melanogaster embryos. Alt-EJ can be defined as a set of Ku70/80 and DNA ligase 4-independent end-joining processes that are typically mutagenic, producing deletions, insertions, and chromosomal rearrangements more frequently than higher-fidelity repair pathways such as classical nonhomologous end joining...
#1Kelly Beagan (Tufts University)H-Index: 3
#2Robin L. Armstrong (Tufts University)H-Index: 1
Last.Mitch McVey (Tufts University)H-Index: 17
view all 8 authors...
Double strand breaks (DSBs) and interstrand crosslinks (ICLs) are toxic DNA lesions that can be repaired through multiple pathways, some of which involve shared proteins. One of these proteins, DNA Polymerase θ (Pol θ), coordinates a mutagenic DSB repair pathway named microhomology-mediated end joining (MMEJ) and is also a critical component for bypass or repair of ICLs in several organisms. Pol θ contains both polymerase and helicase-like domains that are tethered by an unstructured central reg...
#1Jessica L. Alexander (MIT: Massachusetts Institute of Technology)H-Index: 3
#2Kelly Beagan (Tufts University)H-Index: 3
Last.Mitch McVey (Tufts University)H-Index: 17
view all 4 authors...
Abstract Rereplication generates double-strand breaks (DSBs) at sites of fork collisions and causes genomic damage, including repeat instability and chromosomal aberrations. However, the primary mechanism used to repair rereplication DSBs varies across different experimental systems. In Drosophila follicle cells, developmentally regulated rereplication is used to amplify six genomic regions, two of which contain genes encoding eggshell proteins. We have exploited this system to test the roles of...
#1Mitch McVey (Tufts University)H-Index: 17
#2Varandt Y. Khodaverdian (Tufts University)H-Index: 2
Last.Wolf Dietrich HeyerH-Index: 47
view all 5 authors...
Homologous recombination (HR) is a central process to ensure genomic stability in somatic cells and during meiosis. HR-associated DNA synthesis determines in large part the fidelity of the process. A number of recent studies have demonstrated that DNA synthesis during HR is conservative, less processive, and more mutagenic than replicative DNA synthesis. In this review, we describe mechanistic features of DNA synthesis during different types of HR-mediated DNA repair, including synthesis-depende...
#1Kelly Beagan (Tufts University)H-Index: 3
#2Mitch McVey (Tufts University)H-Index: 17
DNA polymerase theta (Pol θ) is an error-prone A-family polymerase that is highly conserved among multicellular eukaryotes and plays multiple roles in DNA repair and the regulation of genome integrity. Studies conducted in several model organisms have shown that Pol θ can be utilized during DNA interstrand crosslink repair and during alternative end-joining repair of double-strand breaks. Recent genetic and biochemical studies have begun to elucidate the unique structural features of Pol θ that ...
1234