Mitch McVey
Tufts University
Homologous recombinationDNA repairMolecular biologyGeneticsBiology
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Publications 41
#1Arpita Jajoo (Tufts University)
#2Catherine Donlon (Tufts University)
Last. Mitch McVey (Tufts University)H-Index: 19
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Sertraline hydrochloride is a commonly prescribed antidepressant medication that acts by amplifying serotonin signaling. Numerous studies have suggested that children of women taking sertraline during pregnancy have an increased risk of developmental defects. Resolving the degree of risk for human fetuses requires comprehensive knowledge of the pathways affected by this drug. We utilized a Drosophila melanogaster model system to assess the effects of sertraline throughout development. Ingestion ...
#1Steven J. Marygold (University of Cambridge)H-Index: 14
#2Helen Attrill (University of Cambridge)H-Index: 4
Last. Masamitsu Yamaguchi (Kyoto Institute of Technology)H-Index: 33
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ABSTRACTDNA synthesis during replication or repair is a fundamental cellular process that is catalyzed by a set of evolutionary conserved polymerases. Despite a large body of research, the DNA poly...
#1Deirdre Cassidy (NEIU: Northeastern Illinois University)
#2Derek G. Epiney (NEIU: Northeastern Illinois University)
Last. Elyse Bolterstein (NEIU: Northeastern Illinois University)H-Index: 1
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Abstract Werner syndrome (WS) is an autosomal recessive progeroid disease characterized by patients' early onset of aging, increased risk of cancer and other age-related pathologies. WS is caused by mutations in WRN, a RecQ helicase that has essential roles responding to DNA damage and preventing genomic instability. While human WRN has both an exonuclease and helicase domain, Drosophila WRNexo has high genetic and functional homology to only the exonuclease domain of WRN. Like WRN-deficient hum...
#1Ece Kocak (Tufts University)H-Index: 1
#2Sarah Dykstra (Tufts University)H-Index: 1
Last. Mitch McVey (Tufts University)H-Index: 19
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PIF1 is a 59 to 39 DNA helicase that can unwind double-stranded DNA and disrupt nucleic acid-protein complexes. In Saccharomyces cerevisiae, Pif1 plays important roles in mitochondrial and nuclear genome maintenance, telomere length regulation, unwinding of G-quadruplex structures, and DNA synthesis during break-induced replication. Some, but not all, of these functions are shared with other eukaryotes. To gain insight into the evolutionarily conserved functions of PIF1, we created pif1 null mut...
#1Elyse BoltersteinH-Index: 1
#2Mitch McVeyH-Index: 19
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#1Brenna M.G. Gormally (Tufts University)H-Index: 3
#2Rory G. Fuller (Tufts University)H-Index: 1
Last. L. Michael Romero (Tufts University)H-Index: 49
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Abstract Corticosterone does not change in consistent ways across species and contexts, making it challenging to use as an indicator of chronic stress. We assessed DNA damage as a potential metric that could be a more integrative stress measurement with direct links to health. We captured free-living house sparrows, took an immediate blood sample, and transferred them to the laboratory, exposing them to the chronic stress of captivity. Biweekly blood and weight samples were then taken for 4 week...
6 CitationsSource
#1Terrence Hanscom (Tufts University)H-Index: 1
#2Varandt Y. Khodaverdian (Tufts University)H-Index: 2
Last. Mitch McVey (Tufts University)H-Index: 19
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Abstract In this chapter, we describe a method for the recovery and analysis of alternative end-joining (alt-EJ) DNA double-strand break repair junctions following I-SceI cutting in Drosophila melanogaster embryos. Alt-EJ can be defined as a set of Ku70/80 and DNA ligase 4-independent end-joining processes that are typically mutagenic, producing deletions, insertions, and chromosomal rearrangements more frequently than higher-fidelity repair pathways such as classical nonhomologous end joining o...
#1Varandt Y. Khodaverdian (Tufts University)H-Index: 2
#2Terrence Hanscom (Tufts University)H-Index: 1
Last. Mitch McVey (Tufts University)H-Index: 19
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9 CitationsSource
#1Kelly Beagan (Tufts University)H-Index: 3
#2Robin L. Armstrong (Tufts University)H-Index: 1
Last. Mitch McVey (Tufts University)H-Index: 19
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Double strand breaks (DSBs) and interstrand crosslinks (ICLs) are toxic DNA lesions that can be repaired through multiple pathways, some of which involve shared proteins. One of these proteins, DNA Polymerase θ (Pol θ), coordinates a mutagenic DSB repair pathway named microhomology-mediated end joining (MMEJ) and is also a critical component for bypass or repair of ICLs in several organisms. Pol θ contains both polymerase and helicase-like domains that are tethered by an unstructured central reg...
13 CitationsSource
#1Varandt Y. Khodaverdian (Tufts University)H-Index: 2
#2Mitch McVey (Tufts University)H-Index: 19