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Gaetana Napolitano
University of Naples Federico II
17Publications
7H-index
104Citations
Publications 17
Newest
From their discovery in biological systems, reactive oxygen species (ROS) have been considered key players in tissue injury for their capacity to oxidize biological macromolecules [...]
Unaccustomed and/or exhaustive exercise generates excessive free radicals and reactive oxygen and nitrogen species leading to muscle oxidative stress-related damage and impaired contractility. Conversely, a moderate level of free radicals induces the body’s adaptive responses. Thus, a low oxidant level in resting muscle is essential for normal force production, and the production of oxidants during each session of physical training increases the body’s antioxidant defenses. Mitochondria, NADPH o...
Abstract The cholesterol metabolite 24(S)-hydroxycholesterol (24S-OHC) allows cholesterol excretion from the brain and was suggested to be critically involved in physiological as well as neurodegenerative processes. It induces on human neuronal cell cultures a dose dependent toxicity associated with increased reactive oxygen species production. Since glial cells play a key role in assisting neuronal function, here we investigated the effects of increased concentrations of 24S-OHC on a glial cell...
#1Gaetana Napolitano (University of Naples Federico II)H-Index: 7
#2Paola Venditti (University of Naples Federico II)H-Index: 26
Last.Claudio Agnisola (University of Naples Federico II)H-Index: 16
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Rapid fluctuations of the oxygen content of both natural and anthropogenic origin are relatively common in freshwater environments. Fish adaptation to these conditions implies tolerance of both low levels of oxygen availability and reoxygenation. Hypoxia tolerance in fish has been widely studied, but the involvement of mitochondria in the response of fish to rapid hypoxia/reoxygenation stress is less known. Zebrafish, a floodplain species, is likely facing significant changes in dissolved oxygen...
Abstract We investigated the effects of thyroid state on the mechanisms underlying rat heart mitochondrial capacity to remove H 2 O 2 produced by an exogenous source. The removal rates were higher in the presence of respiratory substrates independently from thyroid state and were higher in hyperthyroid than in hypothyroid preparations. The thyroid state-linked changes in H 2 O 2 removal rates, mirrored those in H 2 O 2 release rates, showing that endogenous and exogenous H 2 O 2 do not compete f...
#1Gaetana Napolitano (University of Naples Federico II)H-Index: 7
#2Daniela Barone (University of Naples Federico II)H-Index: 5
Last.Paola Venditti (University of Naples Federico II)H-Index: 26
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We studied the effects of adrenaline administration and depletion (induced by reserpine) on rat liver oxidative metabolism. We showed that adrenaline increases, and reserpine decreases aerobic capacity (inferred by cytochrome oxidase activity) in tissue modifying the hepatic content of mitochondrial proteins without changing mitochondrial aerobic capacity. The changes in tissue cytochrome oxidase activity, which agreed with the expression levels of factors involved in mitochondrial biogenesis, s...
#1Paola Venditti (University of Naples Federico II)H-Index: 26
#2Gaetana Napolitano (University of Naples Federico II)H-Index: 7
Last.Sergio Di Meo (University of Naples Federico II)H-Index: 14
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Abstract Continuous exposure of homeothermic animals to low environmental temperatures elicits physiological adaptations necessary for animal survival, which are associated to higher generation of pro-oxidants in thermogenic tissues. It is not known whether intermittent cold exposure (cold training) is able to affect tissue responses to continuous cold exposure. Therefore, we investigated whether rat liver responses to continuous cold exposure of 2 days are modified by cold training (1 h daily f...
#1Paola VendittiH-Index: 26
#2Gaetana NapolitanoH-Index: 7
Last.Sergio Di MeoH-Index: 14
view all 5 authors...
ABSTRACTWe investigated whether reactive oxygen species (ROS) are involved in heart adaptive responses administering a vitamin E-enriched diet to trained rats. Using the homogenates and/or mitochondria from rat hearts we determined the aerobic capacity, tissue level of mitochondrial proteins, and expression of cytochrome c and factors (PGC-1, NRF-1, and NRF-2) involved in mitochondrial biogenesis. We also determined the oxidative damage, glutathione peroxidase (GPX) and reductase activities, glu...
Abstract We investigated thyroid state effect on capacity of rat liver mitochondria to remove exogenously produced H 2 O 2 , determining their ability to decrease fluorescence generated by an H 2 O 2 detector system. The rate of H 2 O 2 removal by both non respiring and respiring mitochondria was increased by hyperthyroidism and decreased by hypothyroidism. However, the rate was higher in the presence of respiratory substrates, in particular pyruvate/malate, indicating a respiration-dependent pr...
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