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Hugo M. Santos
Universidade Nova de Lisboa
142Publications
17H-index
1,229Citations
Publications 142
Newest
#1Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
Last.José Luis Capelo-Martínez (NOVA: Universidade Nova de Lisboa)H-Index: 4
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Abstract A novel analytical approach is proposed to discriminate between solid biopsies of chromophobe renal cell carcinoma (chRCC) and renal oncocytoma (RO). The method comprises the following steps: (i) ultrasonic extraction of proteins from solid biopsies, (ii) protein depletion with acetonitrile, (iii) ultrasonic assisted in-solution digestion using magnetic nanoparticle with immobilized trypsin, (iv) C18 tip-based preconcentration of peptides, (v) sequential extraction of the peptides with ...
#1Cristina dos Santos Mesquita (University of Minho)
#2Pedro Soares-Castro (University of Minho)H-Index: 4
Last.Pedro M. Santos (University of Minho)H-Index: 17
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Abstract In this work, a genotype-phenotype survey of a highly diversified Pseudomonas aeruginosa collection was conducted, aiming to detail pathogen-associated scenarios that clinicians face nowadays. Genetic relation based on RAPD-PCR of 705 isolates, retrieved from 424 patients and several clinical contexts, reported an almost isolate-specific molecular-pattern. Pneumonia-associated isolates HB13 and HB15, clustered in the same RAPD-PCR group, were selected to evaluate the genomic background ...
Abstract We present a method to preserve and process urine proteins for proteomic analysis in a filter aided sample preparation (FASP) format. The method combines concentration of urine proteins in ultrafiltration devices, their thermal stabilization, allowing long term storage of the samples, and filter aided sample preparation. Proteomes of four different urines were preserved during 48 h and 6 months using (i) the classic freeze preservation at −20 °C, (ii) snap-heated freeze-free preservatio...
#1J.E. Araújo (NOVA: Universidade Nova de Lisboa)H-Index: 7
#2Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
Last.J.L. Capelo (NOVA: Universidade Nova de Lisboa)H-Index: 3
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Abstract The adverse effects of air pollution have been long studied in the lung and respiratory systems, but the molecular changes that this causes at the central nervous system level have yet to be fully investigated and understood. To explore the evolution with time of protein expression levels in the brain of rats exposed to particulate matter of different sizes, we carried out two-dimensional gel electrophoresis followed by determination of dysregulated proteins through Coomassie blue stain...
#1Susana Jorge (NOVA: Universidade Nova de Lisboa)H-Index: 3
#2José Luis Capelo (NOVA: Universidade Nova de Lisboa)H-Index: 30
Last.Hugo M. Santos (NOVA: Universidade Nova de Lisboa)H-Index: 17
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An effective three-step proteomics workflow is proposed to overcome the pitfalls caused by polymers present in optimum cutting temperature (OCT)-embedded tissue during its preparation for mass spectrometry analysis. First, the OCT-embedded tissue biopsies are cleaned using ethanol and water in a sequential series of ultrasonic washes in an ultrasound bath (35 kHz ultrasonic frequency, 100% ultrasonic amplitude, 2 min of ultrasonic duty time). Second, a fast ultrasonic-assisted extraction of prot...
#1Luís Pinto (UTAD: University of Trás-os-Montes and Alto Douro)H-Index: 6
#2Carmen Torres (UR: University of La Rioja)H-Index: 51
Last.José Eduardo Pereira (UTAD: University of Trás-os-Montes and Alto Douro)H-Index: 3
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Extended spectrum beta-lactamase (ESBL)-producing Escherichia coli strain C999 was isolated of a patient with urinary tract infection. Previous genotyping indicated that this strain presented a multidrug resistant phenotype and carried the genes of the ESBL CTX-M-15, in addition to those of TEM-1 and OXA-1 enzymes. The whole-cell proteome, and the membrane, cytoplasmic, periplasmic and extracellular sub-proteomes of C999 were obtained in this work by two-dimensional gel electrophoresis followed ...
#1Gonçalo Martins (NOVA: Universidade Nova de Lisboa)
#2Javier Fernández-Lodeiro (NOVA: Universidade Nova de Lisboa)H-Index: 10
Last.Hugo M. Santos (NOVA: Universidade Nova de Lisboa)H-Index: 17
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Abstract The ultimate high-throughput, high robustness and easy-to-handling sample treatment method for label-free shotgun proteomics is presented in this work. It is based on joining the effectiveness of immobilized trypsin at the nanoscale level with the latest technology to deliver ultrasonic energy. The new method can be used to reduce sample preparation time comprising the steps of reduction, alkylation and digestion time to just 15 min without compromising shotgun label-free protein quanti...
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