Hugo M. Santos
Universidade Nova de Lisboa
119Publications
17H-index
1,193Citations
Publications 119
Newest
Published on Jan 1, 2019in Journal of Proteomics 3.72
José Luis Capelo29
Estimated H-index: 29
(Universidade Nova de Lisboa),
Carlos Lodeiro32
Estimated H-index: 32
(Universidade Nova de Lisboa)
+ 1 AuthorsGilberto Igrejas22
Estimated H-index: 22
(University of Lisbon)
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Published on Feb 1, 2019in Dyes and Pigments 3.77
Gonçalo Marcelo2
Estimated H-index: 2
(Universidade Nova de Lisboa),
Sónia M. G. Pires8
Estimated H-index: 8
(University of Aveiro)
+ 7 AuthorsElisabete Oliveira19
Estimated H-index: 19
(Universidade Nova de Lisboa)
Abstract Multifunctional luminescent nanomaterials based on mesoporous silica SBA-16 doped with a ring-opening rhodamine derivative ( RhNHS ) or with a cationic porphyrin bearing imidazolium substituents ( Porph ) were successfully obtained and characterized. The non-immobilized Porph leads to a selective colorimetric detection for Cu 2+ , Pb 2+ and Hg 2+ in HEPES buffer at pH 7, producing colour changes from violet to pink, green and brown, detecting the minimal amount of 3.6, 5.8 and 3.8 ppm b...
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Published on Feb 1, 2019in Dyes and Pigments 3.77
Elisabete Oliveira19
Estimated H-index: 19
(Universidade Nova de Lisboa),
Hugo M. Santos17
Estimated H-index: 17
(Universidade Nova de Lisboa)
+ 6 AuthorsCarlos Lodeiro32
Estimated H-index: 32
(Universidade Nova de Lisboa)
Abstract The synthesis and full characterization of a family of sustainable luminescent nanoparticles, CdTeQDs@MNs, made of quantum dots coated by modified mesoporous silica nanoparticles is reported. The presence of the luminescent QDs allows the imaging of the nanoparticles during cell internalization, as well as the visualization of selective release of doxorubicin inside the cell (via pH stimuli). The modified mesoporous silica nanoparticles lead to pore size improvement allowing better enca...
2 Citations Source Cite
Published on May 1, 2019in Talanta 4.24
Gonçalo Martins (Universidade Nova de Lisboa), Javier Fernández-Lodeiro10
Estimated H-index: 10
(Universidade Nova de Lisboa)
+ 3 AuthorsHugo M. Santos17
Estimated H-index: 17
(Universidade Nova de Lisboa)
Abstract The ultimate high-throughput, high robustness and easy-to-handling sample treatment method for label-free shotgun proteomics is presented in this work. It is based on joining the effectiveness of immobilized trypsin at the nanoscale level with the latest technology to deliver ultrasonic energy. The new method can be used to reduce sample preparation time comprising the steps of reduction, alkylation and digestion time to just 15 min without compromising shotgun label-free protein quanti...
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Published on Apr 1, 2018in Talanta 4.24
J.E. Araújo7
Estimated H-index: 7
(Universidade Nova de Lisboa),
Hugo López-Fernández7
Estimated H-index: 7
(University of Vigo)
+ 7 AuthorsJosé Luis Capelo29
Estimated H-index: 29
(Universidade Nova de Lisboa)
Abstract This study aimed to assess the benefits of dithiothreitol (DTT)-based sample treatment for protein equalization to assess potential biomarkers for bladder cancer. The proteome of plasma samples of patients with bladder carcinoma, patients with lower urinary tract symptoms (LUTS) and healthy volunteers, was equalized with dithiothreitol (DTT) and compared. The equalized proteomes were interrogated using two-dimensional gel electrophoresis and matrix assisted laser desorption ionization t...
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Published on Feb 1, 2018in Talanta 4.24
Susana Jorge3
Estimated H-index: 3
,
J.E. Araújo7
Estimated H-index: 7
+ 4 AuthorsJosé Luis Capelo29
Estimated H-index: 29
Abstract We report on the new microplate horn ultrasonic device as a powerful tool to speed proteomics workflows with unparalleled throughput. 96 complex proteomes were digested at the same time in 4 min. Variables such as ultrasonication time, ultrasonication amplitude, and protein to enzyme ratio were optimized. The "classic" method relying on overnight protein digestion (12 h) and the sonoreactor-based method were also employed for comparative purposes. We found the protein digestion efficien...
2 Citations Source Cite
Published on Jan 1, 2018in ChemistryOpen 2.80
Elisabete Oliveira19
Estimated H-index: 19
(Universidade Nova de Lisboa),
Emilia Bértolo10
Estimated H-index: 10
(Canterbury Christ Church University)
+ 7 AuthorsCarlos Lodeiro32
Estimated H-index: 32
(Universidade Nova de Lisboa)
1 Citations Source Cite
Published on May 1, 2018in Talanta 4.24
João Prates , Gonçalo Martins + 3 AuthorsHugo M. Santos17
Estimated H-index: 17
Abstract In this work we present acetonitrile as a tool to modulate the dynamic range of the proteome of complex samples. Different concentrations of acetonitrile ranging from 15% v/v to 65% v/v were used to modulate the protein content of serum samples from healthy people and patients with lymphoma and myeloma. We show that the proteome above 70 kDa is pelleted as a function of the concentration of acetonitrile and that profiling with PCA or Clustering is only possible using the supernatants ob...
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Published on Dec 1, 2018in Dyes and Pigments 3.77
Ignacio Sánchez5
Estimated H-index: 5
(Complutense University of Madrid),
Cristián Cuerva6
Estimated H-index: 6
(Complutense University of Madrid)
+ 5 AuthorsMercedes Cano19
Estimated H-index: 19
(Complutense University of Madrid)
Abstract Here we describe the preparation, characterisation, liquid crystal properties and photophysical studies of new potassium salts of tetrakis(β-diketonate)europium(III) complexes based on long-chained symmetrical and unsymmetrically substituted 1,3-dialkyloxyphenyl-β-diketones ligands, K[Eu(OO R(n)R(m) ) 4 ] (R(n) = OC 6 H 4 OC n H 2n+1 ; R(m) = OC 6 H 4 OC m H 2m+1 ; n = m = 8, 10, 12, 14, 16; n = 4, 8, m = 12, 16; n = 12, m = 16). All compounds exhibit mesomorphic and luminescence behavi...
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Published on Feb 1, 2018in Talanta 4.24
Jemmyson Romário de Jesus3
Estimated H-index: 3
(Universidade Nova de Lisboa),
Hugo M. Santos17
Estimated H-index: 17
(Universidade Nova de Lisboa)
+ 3 AuthorsJosé Luis Capelo29
Estimated H-index: 29
(Universidade Nova de Lisboa)
Abstract A new ultrafast ultrasonic-based method for shotgun proteomics as well as label-free protein quantification in urine samples is developed. The method first separates the urine proteins using nitrocellulose-based membranes and then proteins are in-membrane digested using trypsin. The enzymatic digestion process is accelerated from overnight to four minutes using a sonoreactor ultrasonic device. Overall, the sample treatment pipeline comprising protein separation, digestion and identifica...
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