Dan Liu
Baylor College of Medicine
Publications 63
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Xiaowei Xie (SYSU: Sun Yat-sen University)H-Index: 7
Last.Zhou SongyangH-Index: 45
view all 12 authors...
The adenine base editor (ABE), capable of catalyzing A•T to G•C conversions, is an important gene editing toolbox. Here, we systematically evaluate genome-wide off-target deamination by ABEs using the EndoV-seq platform we developed. EndoV-seq utilizes Endonuclease V to nick the inosine-containing DNA strand of genomic DNA deaminated by ABE in vitro. The treated DNA is then whole-genome sequenced to identify off-target sites. Of the eight gRNAs we tested with ABE, 2–19 (with an average of 8.0) o...
10 CitationsSource
#1Mafei Xu (BCM: Baylor College of Medicine)H-Index: 4
#2Jun Qin (CAS: Chinese Academy of Sciences)
Last.Sophia Y. Tsai (BCM: Baylor College of Medicine)H-Index: 76
view all 10 authors...
Alternative lengthening of telomeres (ALT) is known to use homologous recombination (HR) to replicate telomeric DNA in a telomerase-independent manner. However, the detailed process remains largely undefined. It was reported that nuclear receptors COUP-TFII and TR4 are recruited to the enriched GGGTCA variant repeats embedded within ALT telomeres, implicating nuclear receptors in regulating ALT activity. Here, we identified a function of nuclear receptors in ALT telomere maintenance that involve...
#1Feng Li (SYSU: Sun Yat-sen University)H-Index: 6
#2Yuanlong Ge (SYSU: Sun Yat-sen University)H-Index: 5
Last.Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 45
view all 4 authors...
Pluripotent stem cells (PSCs) such as embryonic stem cells (ESCs), ESCs derived by somatic cell nuclear transfer (ntESCs), and induced pluripotent stem cells (iPSCs) have unlimited capacity for self-renewal and pluripotency and can give rise to all types of somatic cells. In order to maintain their self-renewal and pluripotency, PSCs need to preserve their telomere length and homeostasis. In recent years, increasing studies have shown that telomere reprogramming is essential for stem cell plurip...
#1Jianxi Zhu (SYSU: Sun Yat-sen University)
#2Mingyang Xu (SYSU: Sun Yat-sen University)
Last.Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 45
view all 9 authors...
#1Tingting Deng (SYSU: Sun Yat-sen University)H-Index: 3
#2Yan Huang (SYSU: Sun Yat-sen University)H-Index: 28
Last.Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 45
view all 11 authors...
5 CitationsSource
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
#2Hongwei Sun (SYSU: Sun Yat-sen University)H-Index: 5
Last.Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 45
view all 13 authors...
4 CitationsSource
#1Feng Li (SYSU: Sun Yat-sen University)H-Index: 6
#2Hyeung Kim (BCM: Baylor College of Medicine)H-Index: 11
Last.Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 45
view all 15 authors...
Summary Telomeres and telomere-binding proteins form complex secondary nucleoprotein structures that are critical for genome integrity but can present serious challenges during telomere DNA replication. It remains unclear how telomere replication stress is resolved during S phase. Here, we show that the BUB3-BUB1 complex, a component in spindle assembly checkpoint, binds to telomeres during S phase and promotes telomere DNA replication. Loss of the BUB3-BUB1 complex results in telomere replicati...
9 CitationsSource
#1Yi Liu (SYSU: Sun Yat-sen University)H-Index: 1
#2Feng Liu (SYSU: Sun Yat-sen University)H-Index: 8
Last.Wenbin Ma (SYSU: Sun Yat-sen University)H-Index: 12
view all 10 authors...
Summary Shwachman-Diamond syndrome (SDS) is a rare pediatric disease characterized by various systemic disorders, including hematopoietic dysfunction. The mutation of Shwachman-Bodian-Diamond syndrome ( SBDS ) gene has been proposed to be a major causative reason for SDS. Although SBDS patients were reported to have shorter telomere length in granulocytes, the underlying mechanism is still unclear. Here we provide data to elucidate the role of SBDS in telomere protection. We demonstrate that SBD...
1 CitationsSource
#1Hyeung Kim (BCM: Baylor College of Medicine)H-Index: 11
#2Feng Li (SYSU: Sun Yat-sen University)H-Index: 6
Last.Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 45
view all 10 authors...
CRISPR/Cas9 technology enables efficient loss-of-function analysis of human genes using somatic cells. Studies of essential genes, however, require conditional knockout (KO) cells. Here, we describe the generation of inducible CRISPR KO human cell lines for the subunits of the telosome/shelterin complex, TRF1, TRF2, RAP1, TIN2, TPP1 and POT1, which directly interact with telomeres or can bind to telomeres through association with other subunits. Homozygous inactivation of several subunits is let...
11 CitationsSource
#1Yuxi Chen (SYSU: Sun Yat-sen University)H-Index: 6
#2Puping Liang (SYSU: Sun Yat-sen University)H-Index: 8
Last.Junjiu HuangH-Index: 19
view all 16 authors...
Spermatids undergo the final steps of maturation during spermiogenesis, a process that necessitates extensive rearrangement of organelles such as the mitochondria. Male infertility has been linked to mitochondrial disorder, for example, hypospermatogenesis and asthenozoospermia. However, the mechanisms that regulate mitochondrial dynamics during spermiogenesis remain largely unknown. We found the glycerol kinase (Gyk)-like proteins glycerol kinase-like 1 (Gykl1) and glycerol kinase 2 (Gk2) were ...
3 CitationsSource