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Dan Liu
Baylor College of Medicine
Molecular biologyTelomereTelomere-binding proteinBiologyCell biology
65Publications
28H-index
3,682Citations
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Publications 65
Newest
#1Yuxi Chen (SYSU: Sun Yat-sen University)H-Index: 6
#2Jiaqi Liu (SYSU: Sun Yat-sen University)H-Index: 1
Last. Zhou SongyangH-Index: 46
view all 10 authors...
Source
#1Yuxi Chen (SYSU: Sun Yat-sen University)H-Index: 6
#2Jiaqi Liu (SYSU: Sun Yat-sen University)H-Index: 1
Last. Zhou SongyangH-Index: 46
view all 10 authors...
Class 2 CRISPR–Cas proteins have been widely developed as genome editing and transcriptional regulating tools. Class 1 type I CRISPR–Cas constitutes ~60% of all the CRISPR–Cas systems. However, only type I–B and I–E systems have been used to control mammalian gene expression and for genome editing. Here we demonstrate the feasibility of using type I–F system to regulate human gene expression. By fusing transcription activation domain to Pseudomonas aeruginosa type I–F Cas proteins, we activate g...
3 CitationsSource
#1Guang Shi (SYSU: Sun Yat-sen University)H-Index: 7
#2Yang Hu (SYSU: Sun Yat-sen University)H-Index: 3
Last. Dan Liu (BCM: Baylor College of Medicine)H-Index: 28
view all 12 authors...
ALT tumor cells often contain abundant DNA damage foci at telomeres and rely on the alternative lengthening of telomeres (ALT) mechanism to maintain their telomeres. How the telomere chromatin is regulated and maintained in these cells remains largely unknown. In this study, we present evidence that heterochromatin protein 1 binding protein 3 (HP1BP3) can localize to telomeres and is particularly enriched on telomeres in ALT cells. HP1BP3 inhibition led to preferential growth inhibition of ALT c...
Source
#1Feng Li (SYSU: Sun Yat-sen University)H-Index: 6
#2Yuanlong Ge (SYSU: Sun Yat-sen University)H-Index: 6
Last. Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 46
view all 4 authors...
Pluripotent stem cells (PSCs) such as embryonic stem cells (ESCs), ESCs derived by somatic cell nuclear transfer (ntESCs), and induced pluripotent stem cells (iPSCs) have unlimited capacity for self-renewal and pluripotency and can give rise to all types of somatic cells. In order to maintain their self-renewal and pluripotency, PSCs need to preserve their telomere length and homeostasis. In recent years, increasing studies have shown that telomere reprogramming is essential for stem cell plurip...
Source
#1Mafei Xu (BCM: Baylor College of Medicine)H-Index: 5
#2Jun Qin (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Sophia Y. Tsai (BCM: Baylor College of Medicine)H-Index: 79
view all 10 authors...
Alternative lengthening of telomeres (ALT) is known to use homologous recombination (HR) to replicate telomeric DNA in a telomerase-independent manner. However, the detailed process remains largely undefined. It was reported that nuclear receptors COUP-TFII and TR4 are recruited to the enriched GGGTCA variant repeats embedded within ALT telomeres, implicating nuclear receptors in regulating ALT activity. Here, we identified a function of nuclear receptors in ALT telomere maintenance that involve...
5 CitationsSource
#1Jianxi Zhu (SYSU: Sun Yat-sen University)H-Index: 1
#2Mingyang Xu (SYSU: Sun Yat-sen University)H-Index: 1
Last. Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 46
view all 9 authors...
2 CitationsSource
#1Tingting Deng (SYSU: Sun Yat-sen University)H-Index: 3
#2Yan Huang (SYSU: Sun Yat-sen University)H-Index: 28
Last. Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 46
view all 11 authors...
: In human cells, telomeres are elongated by the telomerase complex that contains the reverse transcriptase hTERT and RNA template TERC/hTR. Poly(A)-specific ribonuclease (PARN) is known to trim hTR precursors by removing poly(A) tails. However, the precise mechanism of hTR 3' maturation remains largely unknown. Target of Egr1 (TOE1) is an Asp-Glu-Asp-Asp (DEDD) domain containing deadenylase that is mutated in the human disease Pontocerebella Hypoplasia Type 7 (PCH7) and implicated in snRNA and ...
12 CitationsSource
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 10
#2Xi Xie (SYSU: Sun Yat-sen University)H-Index: 14
Last. Zhou SongyangH-Index: 46
view all 11 authors...
The adenine base editor (ABE), capable of catalyzing A•T to G•C conversions, is an important gene editing toolbox. Here, we systematically evaluate genome-wide off-target deamination by ABEs using the EndoV-seq platform we developed. EndoV-seq utilizes Endonuclease V to nick the inosine-containing DNA strand of genomic DNA deaminated by ABE in vitro. The treated DNA is then whole-genome sequenced to identify off-target sites. Of the eight gRNAs we tested with ABE, 2–19 (with an average of 8.0) o...
28 CitationsSource
#1Puping Liang (SYSU: Sun Yat-sen University)H-Index: 10
#2Hongwei Sun (SYSU: Sun Yat-sen University)H-Index: 5
Last. Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 46
view all 13 authors...
12 CitationsSource
#1Feng Li (SYSU: Sun Yat-sen University)H-Index: 6
#2Hyeung Kim (BCM: Baylor College of Medicine)H-Index: 12
Last. Zhou Songyang (SYSU: Sun Yat-sen University)H-Index: 46
view all 15 authors...
Summary Telomeres and telomere-binding proteins form complex secondary nucleoprotein structures that are critical for genome integrity but can present serious challenges during telomere DNA replication. It remains unclear how telomere replication stress is resolved during S phase. Here, we show that the BUB3-BUB1 complex, a component in spindle assembly checkpoint, binds to telomeres during S phase and promotes telomere DNA replication. Loss of the BUB3-BUB1 complex results in telomere replicati...
17 CitationsSource
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