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Wenqin Ying
Chinese Academy of Sciences
GeneGenome editingGeneticsBiologyCRISPR
12Publications
7H-index
299Citations
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Publications 13
Newest
#1Erwei ZuoH-Index: 6
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last. LIYixue (CAS: Chinese Academy of Sciences)H-Index: 57
view all 11 authors...
Base editors hold promise for correcting pathogenic mutations, while substantial single nucleotide variations (SNVs) on both DNA and RNA were generated by cytosine base editors (CBEs). Here we examined possibilities to reduce off-target effects by engineering cytosine deaminases. By screening 24 CBEs harboring various rAPOBEC1 (BE3) or human APOBEC3A (BE3-hA3A) mutations on the ssDNA or RNA binding domain, we found 8 CBE variations could maintain high on-target editing efficiency. Using Genome-w...
1 CitationsSource
#1Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 17 authors...
Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
Source
#1Jin-Jing Li (Fujian Medical University)H-Index: 2
#2Xiang Lin (Fujian Medical University)H-Index: 3
Last. Wan-Jin Chen (Fujian Medical University)H-Index: 11
view all 24 authors...
Source
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Yidi Sun (CAS-MPG Partner Institute for Computational Biology)H-Index: 8
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 10 authors...
Genome editing holds promise for correcting pathogenic mutations. However, it is difficult to determine off-target effects of editing due to single nucleotide polymorphism in individuals. Here, we developed a method named GOTI (Genome-wide Off-target analysis by Two-cell embryo Injection) to detect off-target mutations by editing one blastomere of two-cell mouse embryos using either CRISPR-Cas9 or base editors. Comparison of the whole genome sequences of progeny cells of edited vs. non-edited bl...
66 CitationsSource
#1Guang Yang (CAS: Chinese Academy of Sciences)H-Index: 9
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Yunbo Qiao (GU: Guangzhou University)H-Index: 1
view all 15 authors...
The coactivator-associated arginine methyltransferase CARM1 catalyzes the methylation of histone H3 arginine 17/26 (H3R17/26me) and non-histone proteins at arginine residues to regulate gene transactivation through profiling or Carm1 overexpression assays. However, the direct relationship between H3R17/26me and its causal role in mouse embryo development remains largely unclear. Here, we use rAPOBEC1-XTEN-Cas9n-UGI (BE3) to efficiently introduce a point mutation (R17H) at multiple Hist1/2H3 loci...
Source
#1Hui YangH-Index: 19
#2LIYixueH-Index: 57
Last. Lars M. Steinmetz (Stanford University)H-Index: 56
view all 8 authors...
Genome editing tools including CRISPR/Cas9 and base editors hold great promise for correcting pathogenic mutations. Unbiased genome-wide off-target effects of the editing in mammalian cells is required before clinical applications, but determination of the extent of off-target effects has been difficult due to the existence of single nucleotide polymorphisms (SNPs) in individuals. Here, we developed a method named GOTI (Genome-wide Off-target analysis by Two-cell embryo Injection) to detect off-...
4 CitationsSource
#1He Zhang (CAS: Chinese Academy of Sciences)H-Index: 2
#2Hong Pan (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Zhen Liu (CAS: Chinese Academy of Sciences)H-Index: 48
view all 17 authors...
ABSTRACT In vivo genetic mutation has become a powerful tool for dissecting gene function; however, multi-gene interaction and the compensatory mechanisms involved can make findings from single mutations, at best difficult to interpret, and, at worst, misleading. Hence, it is necessary to establish an efficient way to disrupt multiple genes simultaneously. CRISPR/Cas9-mediated base editing disrupts gene function by converting a protein-coding sequence into a stop codon; this is referred to as CR...
6 CitationsSource
#1Xuan Yao (CAS: Chinese Academy of Sciences)H-Index: 8
#2Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 16 authors...
Summary The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy in which a PCR-amplified or precisely enzyme-cut transgene donor with 800-bp homology arms is injected with Cas9 mRNA and single guide RNA into mouse zygotes. Compared with existing targeting strategies, this method achieved much higher knockin efficiency in mouse embryos, ...
19 CitationsSource
#1Haibo Zhou (CAS: Chinese Academy of Sciences)H-Index: 3
#2J.Q. Liu (CAS: Chinese Academy of Sciences)H-Index: 4
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 22 authors...
Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into f...
44 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Xiaona Huo (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 18 authors...
The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown. Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes. Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosome-specific sites or a cockta...
34 CitationsSource
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