Takeshi Maruyama
Hokkaido University
Publications 25
#1Nobuhiro Kasai (Hokkaido University)H-Index: 2
#2Ailijiang Kadeer (Hokkaido University)H-Index: 2
Last.Yasuyuki Fujita (Hokkaido University)H-Index: 24
view all 7 authors...
Recent studies have revealed that newly emerging RasV12-transformed cells are often apically extruded from the epithelial layer. During this cancer preventive process, cytoskeletal proteins plectin and Epithelial Protein Lost In Neoplasm (EPLIN) are accumulated in RasV12 cells that are surrounded by normal cells, which positively regulate the apical elimination of transformed cells. However, the downstream regulators of the plectin-EPLIN complex remain to be identified. In this study, we have fo...
#1Mikio Takagi (Hokkaido University)
#2Masaya Ikegawa (Dodai: Doshisha University)H-Index: 16
Last.Yasuyuki Fujita (Hokkaido University)H-Index: 24
view all 8 authors...
At the initial stage of carcinogenesis, transformation occurs in single cells within the epithelium. Recent studies have revealed that the newly emerging transformed cells are often apically eliminated from epithelial tissues. However, the underlying molecular mechanisms of this cancer preventive phenomenon still remain elusive. In this study, we first demonstrate that myosin-II accumulates in Src-transformed cells when they are surrounded by normal epithelial cells. Knock-down of the heavy chai...
#1Takeshi Maruyama (Hokkaido University)H-Index: 13
#2Yasuyuki Fujita (Hokkaido University)H-Index: 24
In the multi-cellular community, cells with different properties often compete with each other for survival and space. This process is named cell competition and was originally discovered in Drosophila . Recent studies have revealed that comparable phenomena also occur in mammals under various physiological and pathological conditions. Within the epithelium, normal cells often recognize the presence of the neighboring transformed cells and actively eliminate them from the epithelium; a process t...
#1Ailijiang Kadeer (Hokkaido University)H-Index: 2
#2Takeshi Maruyama (Hokkaido University)H-Index: 13
Last.Yasuyuki Fujita (Hokkaido University)H-Index: 24
view all 10 authors...
#1Shunsuke Kon (Hokkaido University)H-Index: 11
#2Kojiro Ishibashi (Hokkaido University)H-Index: 1
Last.Yuta Yako (Hokkaido University)H-Index: 6
view all 39 authors...
Fujita et al. find that normal epithelial cells induce metabolic changes in adjacent transformed cells, causing their apical extrusion. The effect is conveyed non-cell-autonomously and relies on PDK4-mediated inhibition of mitochondrial function.
#1Novalia Pishesha (MIT: Massachusetts Institute of Technology)H-Index: 6
#2Angelina M. Bilate (MIT: Massachusetts Institute of Technology)H-Index: 18
Last.Stephanie K. Dougan (MIT: Massachusetts Institute of Technology)H-Index: 25
view all 13 authors...
Current therapies for autoimmune diseases rely on traditional immunosuppressive medications that expose patients to an increased risk of opportunistic infections and other complications. Immunoregulatory interventions that act prophylactically or therapeutically to induce antigen-specific tolerance might overcome these obstacles. Here we use the transpeptidase sortase to covalently attach disease-associated autoantigens to genetically engineered and to unmodified red blood cells as a means of in...
#1Sayaka Saitoh (Hokkaido University)H-Index: 3
#2Takeshi Maruyama (Hokkaido University)H-Index: 13
Last.Susumu Ishikawa (Hokkaido University)H-Index: 7
view all 14 authors...
Newly emerging transformed cells are often eliminated from epithelial tissues. Recent studies have revealed that this cancer-preventive process involves the interaction with the surrounding normal epithelial cells; however, the molecular mechanisms underlying this phenomenon remain largely unknown. In this study, using mammalian cell culture and zebrafish embryo systems, we have elucidated the functional involvement of endocytosis in the elimination of RasV12-transformed cells. First, we show th...
#1Takeshi Maruyama (Hokkaido University)H-Index: 13
#2Sayuri Iijima (Hokkaido University)
Last.Yasuyuki Fujita (Hokkaido University)H-Index: 24
view all 3 authors...
#1Guan-Yu Chen (MIT: Massachusetts Institute of Technology)H-Index: 5
#2Zeyang Li (MIT: Massachusetts Institute of Technology)H-Index: 8
Last.Hidde L. Ploegh (MIT: Massachusetts Institute of Technology)H-Index: 115
view all 10 authors...
Peripheral blood can provide valuable information on an individual’s immune status. Cell-based assays typically target leukocytes and their products. Characterization of leukocytes from whole blood requires their separation from the far more numerous red blood cells.1 Current methods to classify leukocytes, such as recovery on antibody-coated beads or fluorescence-activated cell sorting require long sample preparation times and relatively large sample volumes.2 A simple method that enables the c...