Thomas D. Schneider
National Institutes of Health
Publications 108
#1Zhe Sun (NIH: National Institutes of Health)H-Index: 1
#2Cedric Cagliero (NIH: National Institutes of Health)H-Index: 7
Last.Ding Jun Jin (NIH: National Institutes of Health)H-Index: 11
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#1Katherine J. Fenstermacher (UMD: University of Maryland, College Park)H-Index: 1
#2Vasudevan Achuthan (UMD: University of Maryland, College Park)H-Index: 3
Last.Jeffrey J. DeStefano (UMD: University of Maryland, College Park)H-Index: 20
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ABSTRACT DNA polymerases (DNAPs) recognize 3′ recessed termini on duplex DNA and carry out nucleotide catalysis. Unlike promoter-specific RNA polymerases (RNAPs), no sequence specificity is required for binding or initiation of catalysis. Despite this, previous results indicate that viral reverse transcriptases bind much more tightly to DNA primers that mimic the polypurine tract. In the current report, primer sequences that bind with high affinity to Taq and Klenow polymerases were identified u...
#1Zhong Qian (LMB: Laboratory of Molecular Biology)H-Index: 3
#2Andrei Trostel (LMB: Laboratory of Molecular Biology)H-Index: 6
Last.Sankar Adhya (LMB: Laboratory of Molecular Biology)H-Index: 55
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The regulatory protein, GalR, is known for controlling transcription of genes related to D-galactose metabolism in Escherichia coli. Here, using a combination of experimental and bioinformatic approaches, we identify novel GalR binding sites upstream of several genes whose function is not directly related to D-galactose metabolism. Moreover, we do not observe regulation of these genes by GalR under standard growth conditions. Thus, our data indicate a broader regulatory role for GalR, and sugges...
This issue publishes the proceedings of the 2015 CCP4 Study Weekend on Advances in Experimental Phasing, held on 8–9 January 2015 at the East Midlands Conference Centre of the University of Nottingham, UK. Crystallographic phasing using experimental approaches remains an important, and often the only successful, route for solving crystal structures, especially for structures with novel folds. These are often large and complex assemblies diffracting to limited resolution, but also those likely to...
#1Kristen M. Pluchino (NIH: National Institutes of Health)H-Index: 8
#2Dominic Esposito (Leidos)H-Index: 19
Last.Ian A. Pringle (University of Oxford)H-Index: 16
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The efflux transporter P-glycoprotein (P-gp) is an important mediator of various pharmacokinetic parameters, being expressed at numerous physiological barriers and also in multidrug-resistant cancer cells. Molecular cloning of homologous cDNAs is an important tool for the characterization of functional differences in P-gp between species. However, plasmids containing mouse mdr1a cDNA display significant genetic instability during cloning in bacteria, indicating that mdr1a cDNA may be somehow tox...