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Jiun-Yan Wu
National Chung Hsing University
6Publications
2H-index
22Citations
Publications 6
Newest
#1Jiun-Yan Wu (NCHU: National Chung Hsing University)H-Index: 2
#2Jen-Hung Liao (Council of Agriculture)
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 23
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In this study, the precursor effect for iturin A production was quantitatively analyzed. A strain identified as Bacillus amyloliquefaciens BPD1 (Ba-BPD1) was selected due to its ability to produce iturin A. The enhancement of iturin A production in a submerged culture was tested using various additives, including palmitic acid, oils, and complex amino acids. Among these, complex amino acids triggered the highest yield at 559 mg/L. The respective amino acids that contribute to the structure of it...
#1Shu-Chen Kan (NCHU: National Chung Hsing University)H-Index: 5
#2Chi-Ming Chen (NCHU: National Chung Hsing University)H-Index: 2
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 23
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Abstract In this study, six E. coli strains, which harboring enhanced green fluorescence protein (EGFP), an intein (INT) and ice nucleation protein (INP), were used to express the EGFP protein. The effect of E. coli strains on the surfaced displayed EGFP via INP-INT system was first investigated. Among these strains, E. coli JM109(DE3) was found the best strain for EGFP production (67.9 mg/L), two-fold as compared to that produced by E. coli DH1(DE3). It was concluded that lon mutation could sta...
#1Chia-Chi Lin (NCHU: National Chung Hsing University)H-Index: 5
#2Tzu-Tsen Liu (NCHU: National Chung Hsing University)H-Index: 2
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 23
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In this study, the cell surface expression system was the first time used to directly produce extracellular enzyme. In the plasmid construction, the truncated ice nucleation protein (INP) was fused with intein (INT) and target protein, d -hydantoinase ( DHT ase), to form the INP-INT-DHTase gene. The plasmid containing this gene was transformed into Escherichia coli ER2566 cells. The gene construct enables the expression of INP-INT-DHTase fusion protein, which might anchor on cell membrane surfac...
#1Jiun-Yan Wu (NCHU: National Chung Hsing University)H-Index: 2
#2Chih-I ChenH-Index: 11
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 23
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In this study, three approaches for protein production were devised to enhance the efficiencies in yielding high purity protein via surface display system. A plasmid carrying enhanced green fluorescent protein (EGFP), an intein (INT) and ice nucleation protein (INP) was constructed to produce EGFP via surface display in Escherichia coli. To obtain high purity of the produced EGFP, several procedures, including osmotic shock, surfactant addition and cell disruption were employed. Among these appr...
#1Chi-Zong Zang (NCHU: National Chung Hsing University)H-Index: 4
#2Yaw-Nan Chang (NFU: National Formosa University)H-Index: 1
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 23
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Abstract Fungichromin (FC), a polyene macrolide antibiotic, was found to be useful in the treatment of resistance for plant diseases such as Rhizoctonia damping-off of cabbage and tomato late blight. A novel strain, Streptomyces padanus PMS-702, was employed to study the cultivation conditions for FC production. When using linoleic acid as an additive, a negative effect on FC production was observed. On the contrary, when using oleic acid in concentration of 0.01 M (2.82 g/L), a 3.5-fold enhance...
#1Jiun-Yan Wu (NCHU: National Chung Hsing University)H-Index: 2
#2Tsung-Yu Tsai (NCHU: National Chung Hsing University)H-Index: 1
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 23
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Abstract In this study, a novel recombinant protein production system was developed by constructing a protein chimera with a self-cleavage segment along with a cell surface display segment to finally produce a model protein entity ( i.e. , enhanced green fluorescent protein, EGFP). In the plasmid construction, the EGFP gene was fused to genes of a self-cleaving intein (INT) and an ice nucleation protein (INP) which can anchor on the cell membrane. In the cultivation of recombinant Escherichia co...
#1Hua-Bing Chen (NCHU: National Chung Hsing University)H-Index: 4
#2Jiun-Yan Wu (NCHU: National Chung Hsing University)H-Index: 2
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 23
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Abstract Light-emitting diodes (LEDs) with various light wavelengths (red, yellow, green, blue and white) and various levels of light intensity (750, 1500 and 3000 μmol m −2 s −1 ) are applied to study the effects of light sources on chlorophyll a (Chl) and phycocyanin (Phy) production of Spirulina platensis . Logistic rate equations are used to depict the kinetic behavior of algal growth and pigments formation under various light sources and intensities. According to the regression results, a r...
#1Jiun-Yan Wu (NCHU: National Chung Hsing University)H-Index: 2
#2Jenn-Wen Huang (NCHU: National Chung Hsing University)H-Index: 1
Last.Yung-Chuan Liu (NCHU: National Chung Hsing University)H-Index: 23
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Abstract A novel strain, Streptomyces padanus PMS-702, was employed to produce fungichromin (FC, a polyene macrolide antibiotic) in a shake flask cultivation. In comparing the effects of various carbon and nitrogen sources on PMS-702 cultivation, it was found that glucose and soybean meal medium yielded the highest FC within 2 days. Factors such as medium composition, cultivation temperatures, and initial pH were optimized for FC production with response surface methodology (RSM). The optimal cu...
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