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Christopher M. Hale
Amgen
17Publications
13H-index
1,205Citations
Publications 17
Newest
#1Antony Chi Shing Chan (California Institute of Technology)
#2Jinho Kim (California Institute of Technology)
Last.Changhuei Yang (California Institute of Technology)H-Index: 44
view all 0 authors...
We report the implementation of a parallel microscopy system (96 Eyes) that is capable of simultaneous imaging of all wells on a 96-well plate. The optical system consists of 96 microscopy units, where each unit is made out of a four element objective, made through a molded injection process, and a low cost CMOS camera chip. By illuminating the sample with angle varying light and applying Fourier Ptychography, we can improve the effective brightfield imaging numerical aperture of the objectives ...
#1Peter W. Zandstra (U of T: University of Toronto)H-Index: 41
#2Nimalan Thavandiran (U of T: University of Toronto)H-Index: 10
Last.Bo Sun (UHN: University Health Network)
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To accelerate the cardiac drug discovery pipeline, we set out to develop a platform that would be amenable to standard multiwell-plate manipulations and be capable of quantifying tissue-level functions such as contractile force. We report a 96-well-based array of 3D human pluripotent stem cell (hPSC)-derived cardiac microtissues - termed Cardiac MicroRings (CaMiRi) - in custom printed multiwell plates capable of contractile force measurement. Within each well, two elastomeric microcantilevers ar...
#1Antony Chi Shing Chan (California Institute of Technology)
#2Jinho Kim (California Institute of Technology)
Last.Changhuei Yang (California Institute of Technology)
view all 8 authors...
We report the implementation of a parallel microscopy system (96 Eyes) that is capable of simultaneous imaging of all wells on a 96 well plate. The optical system consists of 96 microscopy units, where each unit is made out of a four element objective, made through a molded injection process, and a low cost CMOS camera chip. By illuminating the sample with angle varying light and applying Fourier Ptychography, we can improve the effective brightfield imaging NA of the objectives from 0.23 to 0.3...
#1Patrick J. Collins (Amgen)H-Index: 1
#2Christopher M. Hale (Amgen)H-Index: 13
Last.Han Xu (Amgen)H-Index: 1
view all 3 authors...
Abstract Within the short few years since the report of its application in precise genome editing, CRISPR technology has become the method of choice to modify and modulate gene expression in biomedical research and therapeutic development. Subsequently, a variety of research, diagnostic, and therapeutic tools have been developed based upon CRISPR’s mechanism of action. Such tools have helped to deepen the understanding of fundamental biology and broaden the horizon in the search for treatments f...
#1Christopher M. Hale (Amgen)H-Index: 13
#2Qingwen Cheng (Amgen)H-Index: 1
Last.Holly J. Carlisle (Amgen)H-Index: 2
view all 9 authors...
ABSTRACTAutophagy is the primary process for recycling cellular constituents through lysosomal degradation. In addition to nonselective autophagic engulfment of cytoplasm, autophagosomes can recognize specific cargo by interacting with ubiquitin-binding autophagy receptors such as SQSTM1/p62 (sequestosome 1). This selective form of autophagy is important for degrading aggregation-prone proteins prominent in many neurodegenerative diseases. We carried out a high content image-based siRNA screen (...
#1Shyam B. Khatau (Johns Hopkins University)H-Index: 12
#2Ryan J. Bloom (Stanford University)H-Index: 5
Last.Christopher M. Hale (Johns Hopkins University)H-Index: 13
view all 13 authors...
Cells often migrate in vivo in an extracellular matrix that is intrinsically three-dimensional (3D) and the role of actin filament architecture in 3D cell migration is less well understood. Here we show that, while recently identified linkers of nucleoskeleton to cytoskeleton (LINC) complexes play a minimal role in conventional 2D migration, they play a critical role in regulating the organization of a subset of actin filament bundles – the perinuclear actin cap - connected to the nucleus throug...
#1Pei Hsun Wu (Johns Hopkins University)H-Index: 17
#2Christopher M. Hale (Johns Hopkins University)H-Index: 13
Last.Denis Wirtz (Johns Hopkins University)H-Index: 68
view all 6 authors...
High-throughput ballistic injection nanorheology is a method for the quantitative study of cell mechanics. Cell mechanics are measured by ballistic injection of submicron particles into the cytoplasm of living cells and tracking the spontaneous displacement of the particles at high spatial resolution. The trajectories of the cytoplasm-embedded particles are transformed into mean-squared displacements, which are subsequently transformed into frequency-dependent viscoelastic moduli and time-depend...
#1Christopher M. Hale (Johns Hopkins University)H-Index: 13
#2Wei Chiang Chen (Johns Hopkins University)H-Index: 11
Last.Denis Wirtz (Johns Hopkins University)H-Index: 68
view all 6 authors...
In several migratory cells, the microtubule-organizing center (MTOC) is repositioned between the leading edge and nucleus, creating a polarized morphology. Although our understanding of polarization has progressed as a result of various scratch-wound and cell migration studies, variations in culture conditions required for such assays have prevented a unified understanding of the intricacies of MTOC and nucleus positioning that result in cell polarization. Here, we employ a new SMRT (for sparse,...
#1Alfredo Celedon (Johns Hopkins University)H-Index: 9
#2Christopher M. Hale (Johns Hopkins University)H-Index: 13
Last.Denis Wirtz (Johns Hopkins University)H-Index: 68
view all 3 authors...
The organization of chromatin in the cell nucleus is crucial for gene expression regulation. However, physically probing the nuclear interior is challenging because high forces have to be applied using minimally invasive techniques. Here, magnetic nanorods embedded in the nucleus of living cells are subjected to controlled rotational forces, producing micron-sized displacements in the nuclear interior. The resulting time-dependent rotation of the nanorods is analyzed in terms of viscoelastic par...
#1Stephanie I. FraleyH-Index: 12
#2Christopher M. HaleH-Index: 13
Last.Denis Wirtz (Johns Hopkins University)H-Index: 68
view all 6 authors...
Limited progress in our understanding of molecular cell functions in 3D is due, in part, to the lack of quantitative assays to probe cells in the physiological three-dimensional (3D) milieu. We have recently developed quantitative methods to probe the micromechanical properties of live cells inside a 3D matrix and monitor the dynamics of localized matrix remodeling during 3D cell motility. Conventional biophysical methods are unsuitable for cells fully embedded inside a matrix because these meth...
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