Christopher M. Hale
Amgen
17Publications
13H-index
1,181Citations
Publications 17
Newest
Published on Feb 13, 2019in bioRxiv
Antony Chi Shing Chan (California Institute of Technology), Jinho Kim (California Institute of Technology)+ 5 AuthorsChanghuei Yang (California Institute of Technology)
We report the implementation of a parallel microscopy system (96 Eyes) that is capable of simultaneous imaging of all wells on a 96 well plate. The optical system consists of 96 microscopy units, where each unit is made out of a four element objective, made through a molded injection process, and a low cost CMOS camera chip. By illuminating the sample with angle varying light and applying Fourier Ptychography, we can improve the effective brightfield imaging NA of the objectives from 0.23 to 0.3...
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Published on Mar 4, 2019in bioRxiv
Peter W. Zandstra39
Estimated H-index: 39
(University of Toronto),
Nimalan Thavandiran10
Estimated H-index: 10
(University of Toronto)
+ 20 AuthorsGeorge Graham
To accelerate the cardiac drug discovery pipeline, we set out to develop a platform that would be amenable to standard multiwell-plate manipulations and be capable of quantifying tissue-level functions such as contractile force. We report a 96-well-based array of 3D human pluripotent stem cell (hPSC)-derived cardiac microtissues - termed Cardiac MicroRings (CaMiRi) - in custom printed multiwell plates capable of contractile force measurement. Within each well, two elastomeric microcantilevers ar...
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Published on Nov 1, 2017in Pharmacological Research 4.90
Patrick J. Collins2
Estimated H-index: 2
(Amgen),
Christopher M. Hale13
Estimated H-index: 13
(Amgen),
Han Xu1
Estimated H-index: 1
(Amgen)
Abstract Within the short few years since the report of its application in precise genome editing, CRISPR technology has become the method of choice to modify and modulate gene expression in biomedical research and therapeutic development. Subsequently, a variety of research, diagnostic, and therapeutic tools have been developed based upon CRISPR’s mechanism of action. Such tools have helped to deepen the understanding of fundamental biology and broaden the horizon in the search for treatments f...
3 Citations Source Cite
Published on Apr 2, 2016in Autophagy 11.10
Christopher M. Hale13
Estimated H-index: 13
(Amgen),
Qingwen Cheng1
Estimated H-index: 1
(Amgen)
+ 6 AuthorsHolly J. Carlisle2
Estimated H-index: 2
(Amgen)
ABSTRACTAutophagy is the primary process for recycling cellular constituents through lysosomal degradation. In addition to nonselective autophagic engulfment of cytoplasm, autophagosomes can recognize specific cargo by interacting with ubiquitin-binding autophagy receptors such as SQSTM1/p62 (sequestosome 1). This selective form of autophagy is important for degrading aggregation-prone proteins prominent in many neurodegenerative diseases. We carried out a high content image-based siRNA screen (...
15 Citations Source Cite
Published on Dec 1, 2012in Scientific Reports 4.12
Shyam B. Khatau12
Estimated H-index: 12
(Johns Hopkins University),
Ryan J. Bloom7
Estimated H-index: 7
(Stanford University)
+ 10 AuthorsChristopher M. Hale13
Estimated H-index: 13
(Johns Hopkins University)
Cells often migrate in vivo in an extracellular matrix that is intrinsically three-dimensional (3D) and the role of actin filament architecture in 3D cell migration is less well understood. Here we show that, while recently identified linkers of nucleoskeleton to cytoskeleton (LINC) complexes play a minimal role in conventional 2D migration, they play a critical role in regulating the organization of a subset of actin filament bundles – the perinuclear actin cap - connected to the nucleus throug...
84 Citations Source Cite
Published on Jan 1, 2012in Nature Protocols 12.42
Pei Hsun Wu16
Estimated H-index: 16
(Johns Hopkins University),
Christopher M. Hale13
Estimated H-index: 13
(Johns Hopkins University)
+ 3 AuthorsDenis Wirtz68
Estimated H-index: 68
(Johns Hopkins University)
High-throughput ballistic injection nanorheology is a method for the quantitative study of cell mechanics. Cell mechanics are measured by ballistic injection of submicron particles into the cytoplasm of living cells and tracking the spontaneous displacement of the particles at high spatial resolution. The trajectories of the cytoplasm-embedded particles are transformed into mean-squared displacements, which are subsequently transformed into frequency-dependent viscoelastic moduli and time-depend...
33 Citations Source Cite
Published on Dec 15, 2011in Journal of Cell Science 4.40
Christopher M. Hale13
Estimated H-index: 13
(Johns Hopkins University),
Wei Chiang Chen10
Estimated H-index: 10
(Johns Hopkins University)
+ 3 AuthorsDenis Wirtz68
Estimated H-index: 68
(Johns Hopkins University)
In several migratory cells, the microtubule-organizing center (MTOC) is repositioned between the leading edge and nucleus, creating a polarized morphology. Although our understanding of polarization has progressed as a result of various scratch-wound and cell migration studies, variations in culture conditions required for such assays have prevented a unified understanding of the intricacies of MTOC and nucleus positioning that result in cell polarization. Here, we employ a new SMRT (for sparse,...
22 Citations Source Cite
Published on Oct 1, 2011in Biophysical Journal 3.50
Alfredo Celedon8
Estimated H-index: 8
(Johns Hopkins University),
Christopher M. Hale13
Estimated H-index: 13
(Johns Hopkins University),
Denis Wirtz68
Estimated H-index: 68
(Johns Hopkins University)
The organization of chromatin in the cell nucleus is crucial for gene expression regulation. However, physically probing the nuclear interior is challenging because high forces have to be applied using minimally invasive techniques. Here, magnetic nanorods embedded in the nucleus of living cells are subjected to controlled rotational forces, producing micron-sized displacements in the nuclear interior. The resulting time-dependent rotation of the nanorods is analyzed in terms of viscoelastic par...
33 Citations Source Cite
Published on Jul 1, 2010in Nucleus 2.21
Shyam B. Khatau12
Estimated H-index: 12
(Johns Hopkins University),
Dong Hwee Kim13
Estimated H-index: 13
(Johns Hopkins University)
+ 2 AuthorsDenis Wirtz68
Estimated H-index: 68
(Johns Hopkins University)
We recently demonstrated the existence of a previously uncharacterized subset of actomyosin fibers that form the perinuclear actin cap, a cytoskeletal structure that tightly wraps around the nucleus of a wide range of somatic cells. Fibers in the actin cap are distinct from well-characterized, conventional actin fibers at the basal and cortical surfaces of adherent cells in their subcellular location, internal organization, dynamics, ability to generate contractile forces, response to cytoskelet...
41 Citations Source Cite
Published on Dec 1, 2010in Biophysical Journal 3.50
Brian R. Daniels10
Estimated H-index: 10
(Johns Hopkins University),
Christopher M. Hale13
Estimated H-index: 13
(Johns Hopkins University)
+ 4 AuthorsDenis Wirtz68
Estimated H-index: 68
(Johns Hopkins University)
Embryonic and adult fibroblasts can be returned to pluripotency by the expression of reprogramming genes. Multiple lines of evidence suggest that these human induced pluripotent stem (hiPS) cells and human embryonic stem (hES) cells are behaviorally, karyotypically, and morphologically similar. Here we sought to determine whether the physical properties of hiPS cells, including their micromechanical properties, are different from those of hES cells. To this end, we use the method of particle tra...
27 Citations Source Cite
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