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Sojung Kim
Seoul National University
21Publications
10H-index
1,979Citations
Publications 21
Newest
Published on Mar 1, 2018in Genome Research 10.10
Sojung Kim10
Estimated H-index: 10
,
Taeyoung Koo14
Estimated H-index: 14
+ 5 AuthorsJin-Soo Kim52
Estimated H-index: 52
(SNU: Seoul National University)
20 Citations Source Cite
Published on Dec 1, 2017in Genome Biology 13.21
Sojung Kim10
Estimated H-index: 10
,
Taegeun Bae2
Estimated H-index: 2
(UST: Korea University of Science and Technology)
+ 1 AuthorsJin-Soo Kim52
Estimated H-index: 52
(UST: Korea University of Science and Technology)
We report that engineered Cas9 variants with improved specificity—eCas9-1.1 and Cas9-HF1—are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5’ terminus, relative to target DNA sequences. Because the nucleotide at the 5’ end of sgRNAs, expressed under the control of the commonly-used U6 promoter, is fixed to a guanine, these attenuated Cas9 variants are not useful at many target sites. By using sgRNAs with matched 5’ nucleotides, produced...
12 Citations Source Cite
Published on Oct 1, 2017in Stem Cell Research 3.90
Kyung-Ok Uhm1
Estimated H-index: 1
,
Gue Youn Go1
Estimated H-index: 1
+ 6 AuthorsSoo Kyung Koo16
Estimated H-index: 16
Abstract We generated human induced pluripotent stem cells (KSCBi002-B and KSCBi002-B-1) from the dermal fibroblasts of a donor using a modified RNA-based gene delivery method. According to GTG-banding analysis, the generated KSCBi002-B line has a cytogenetic abnormality (46,XY, t(1;4)(q21;q25)) that is distinct from that of the donor, whereas KSCBi002-B-1 has a normal karyotype (46,XY). These cell lines can be useful as a model for characterizing the hiPSCs generated by a non-viral and non-inte...
Source Cite
Published on May 1, 2017in Stem Cell Research 3.90
Kyung-Ok Uhm1
Estimated H-index: 1
,
Sojung Kim10
Estimated H-index: 10
+ 6 AuthorsSoo Kyung Koo16
Estimated H-index: 16
Abstract We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts using a Sendai virus (SeV)-based gene delivery method. The generated hiPSC line, KSCBi002-A, has a normal karyotype (46,XY). The pluripotency and differentiation capacity were characterized by comparison with those of a human embryonic stem cell line. This cell line is registered and available from the National Stem Cell Bank, Korea National Institute of Health.
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Published on Mar 1, 2017in Stem Cell Research 3.90
Sojung Kim10
Estimated H-index: 10
(CBNU: Chungbuk National University),
Omer Habib2
Estimated H-index: 2
(SNU: Seoul National University)
+ 3 AuthorsJung Hyun Kim43
Estimated H-index: 43
Kelch-like ECH-associated protein 1 (keap1) is a cysteine-rich protein that interacts with transcription factor Nrf2 in a redox-sensitive manner, leading to the degradation of Nrf2 (Kim et al., 2014a). Disruption of Keap1 results in the induction of Nrf2-related signaling pathways involving the expression of a set of anti-oxidant and anti-inflammatory genes. We generated biallelic mutants of the Keap1 gene using a CRISPR-Cas9 genome editing method in the H9 human embryonic stem cell (hESC). The ...
2 Citations Source Cite
Published on Jan 1, 2017in Blood Research
Sojung Kim10
Estimated H-index: 10
(CBNU: Chungbuk National University),
Jiwon Jung9
Estimated H-index: 9
+ 3 Authorsand Jung-Hyun Kim1
Estimated H-index: 1
2 Citations Source Cite
Published on Sep 1, 2016in Stem Cell Research 3.90
Sojung Kim10
Estimated H-index: 10
(CBNU: Chungbuk National University),
Omer Habib2
Estimated H-index: 2
(SNU: Seoul National University)
+ 3 AuthorsJung Hyun Kim43
Estimated H-index: 43
Bag1 transcribes a multifunctional protein that participates in many important biological processes such as cell apoptosis, proliferation, differentiation and embryo development. Despite numerous published studies, the role of Bag1 in the context of embryonic stem (ES) cells, has not been explored. To investigate the function of Bag1 in ES cells, we generated mutant Bag1−/− ES cells using the CRISPR/Cas9 system. We established that the Bag1 double knockout ES cell line maintained their pluripote...
5 Citations Source Cite
Published on Mar 1, 2016in Genome Research 10.10
Daesik Kim12
Estimated H-index: 12
(SNU: Seoul National University),
Sojung Kim10
Estimated H-index: 10
(SNU: Seoul National University)
+ 2 AuthorsJin-Soo Kim52
Estimated H-index: 52
(SNU: Seoul National University)
We present multiplex Digenome-seq to profile genome-wide specificities of up to 11 CRISPR-Cas9 nucleases simultaneously, saving time and reducing cost. Cell-free human genomic DNA was digested using multiple sgRNAs combined with the Cas9 protein and then subjected to whole-genome sequencing. In vitro cleavage patterns, characteristic of on- and off-target sites, were computationally identified across the genome using a new DNA cleavage scoring system. We found that many false-positive, bulge-typ...
75 Citations Source Cite
Published on Nov 1, 2015in Nature Protocols 12.42
Jia Liu12
Estimated H-index: 12
(ShanghaiTech University),
Thomas Gaj22
Estimated H-index: 22
(UIUC: University of Illinois at Urbana–Champaign)
+ 6 AuthorsCarlos F. Barbas96
Estimated H-index: 96
(Scripps Research Institute)
In this protocol, the authors describe methods for the direct delivery of ZFN, TALEN and Cas9 nuclease proteins into cells for efficient and targeted genome editing.
52 Citations Source Cite
Published on Jun 1, 2014in Genome Research 10.10
Sojung Kim10
Estimated H-index: 10
(SNU: Seoul National University),
Daesik Kim12
Estimated H-index: 12
(SNU: Seoul National University)
+ 2 AuthorsJin-Soo Kim52
Estimated H-index: 52
(SNU: Seoul National University)
RNA-guided engineered nucleases (RGENs) derived from the prokaryotic adaptive immune system known as CRISPR (clustered, regularly interspaced, short palindromic repeat)/Cas (CRISPR-associated) enable genome editing in human cell lines, animals, and plants, but are limited by off-target effects and unwanted integration of DNA segments derived from plasmids encoding Cas9 and guide RNA at both on-target and off-target sites in the genome. Here, we deliver purified recombinant Cas9 protein and guide...
578 Citations Source Cite
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