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Karl F. Houben
University of California, Los Angeles
6Publications
5H-index
225Citations
Publications 6
Newest
#1Karl F. Houben (UCLA: University of California, Los Angeles)H-Index: 5
#2Kambiz Dardashti (UCLA: University of California, Los Angeles)H-Index: 2
Last.Bruce D. Howard (UCLA: University of California, Los Angeles)H-Index: 23
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Wild type PC12 pheochromocytoma cells express a Na+-dependent norepinephrine transporter that operates in the uptake of catecholamines. In addition to the previously described Na+-dependent system A for the uptake of α-amino-isobutyric acid and system Gly for glycine, we have identified two other Na+-dependent transporter systems for amino acid uptake in these cells: 1) system β for β-alanine and taurine; and 2) a system for creatine. Uptake of α-amino-isobutyric acid, glycine, β-alanine, and cr...
Abstract Wild type PC12 pheochromocytoma cells express a Na(+)-dependent norepinephrine transporter that operates in the uptake of catecholamines, including dopamine. This transporter is not expressed in two spontaneously occurring flat cell variants of PC12 or in two other flat cell variants whose phenotype was induced by expression of the Wnt-1 oncogene. However, each of the flat cell variants, including those that express Wnt-1, exhibit a Na(+)-dependent, Cl(-)-independent glutamate/aspartate...
#1Michael F. Dunn (UCR: University of California, Riverside)H-Index: 34
#2Valentin Aguilar (UCR: University of California, Riverside)H-Index: 2
Last.Melinda Roy (Novo Nordisk)H-Index: 1
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The bacterial tryptophan synthase bienzyme complexes (with subunit composition alpha 2 beta 2) catalyze the last two steps in the biosynthesis of L-tryptophan. For L-tryptophan synthesis, indole, the common metabolite, must be transferred by some mechanism from the alpha-catalytic site to the beta-catalytic site. The X-ray structure of the Salmonella typhimurium tryptophan synthase shows the catalytic sites of each alpha-beta subunit pair are connected by a 25-30 A long tunnel [Hyde, C. C., Ahme...
The reactions of L-histidine (L-His) and L-tryptophan (L-Trp) with the alpha 2 beta 2 complex of Escherichia coli tryptophan synthase are introduced as probes both of beta-subunit catalysis and of ligand-mediated alpha-beta allosteric interactions. Binding of DL-alpha-glycerol 3-phosphate (GP), an analogue of 3-indole-D-glycerol 3'-phosphate (IGP), to the alpha-catalytic site increases the affinity of alpha 2 beta 2 for L-His 4.5-fold and the affinity for L-Trp 17-fold and brings about a redistr...
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