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Mayra A. Machuca
Discovery Institute
Periplasmic spaceHelicobacter pyloriChemotaxisBiochemistryBiology
16Publications
5H-index
100Citations
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Publications 16
Newest
Last. Anna RoujeinikovaH-Index: 19
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#1Mohammad Firoz Khan (DI: Discovery Institute)H-Index: 6
#1Mohammad Firoz Khan (DI: Discovery Institute)H-Index: 4
Last. Anna RoujeinikovaH-Index: 19
view all 7 authors...
Chemotaxis is an important virulence factor of the foodborne pathogen Campylobacter jejuni. Inactivation of chemoreceptor Tlp3 reduces the ability of C. jejuni to invade human and chicken cells and to colonise the jejunal mucosa of mice. Knowledge of the structure of the ligand-binding domain (LBD) of Tlp3 in complex with its ligands is essential for a full understanding of the molecular recognition underpinning chemotaxis. To date, the only structure in complex with a signal molecule is Tlp3 LB...
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#1Mohammad M. Rahman (Monash University, Clayton campus)H-Index: 2
#2Mayra A. Machuca (Monash University, Clayton campus)H-Index: 5
Last. Anna Roujeinikova (Monash University, Clayton campus)H-Index: 19
view all 6 authors...
ABSTRACT The gastric pathogen Helicobacter pylori has limited ability to use carbohydrates as a carbon source, relying instead on exogenous amino acids and peptides. Uptake of certain peptides by H. pylori requires an ATP binding cassette (ABC) transporter annotated dipeptide permease (Dpp). The transporter specificity is determined by its cognate substrate-binding protein DppA, which captures ligands in the periplasm and delivers them to the permease. Here, we show that, unlike previously chara...
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#1Mohammad M. RahmanH-Index: 2
#2Mayra A. MachucaH-Index: 5
Last. Anna RoujeinikovaH-Index: 19
view all 6 authors...
Source
#1Mohammad M. RahmanH-Index: 2
#2Mayra A. MachucaH-Index: 5
Last. Anna RoujeinikovaH-Index: 19
view all 6 authors...
Source
#1Mohammad M. Rahman (DI: Discovery Institute)H-Index: 2
#2Mayra A. Machuca (DI: Discovery Institute)H-Index: 5
Last. Anna Roujeinikova (Monash University)H-Index: 19
view all 3 authors...
: Periplasmic binding proteins (PBPs) of Gram-negative bacteria sense essential nutrients and mediate their uptake by ATP-binding cassette (ABC) transporters. The gene for a PBP of H. pylori SS1, annotated as GlnH, is located within the glnPQH operon encoding an ABC importer system. In this study, GlnH has been expressed in E. coli and purified to > 98% homogeneity. The recombinant protein was folded according to the circular dichroism (CD) analysis and behaved as a monomer in solution. Crystals...
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#1Kar Lok Chan (DI: Discovery Institute)
#2Mayra A. Machuca (DI: Discovery Institute)H-Index: 5
Last. Anna Roujeinikova (Monash University)H-Index: 19
view all 6 authors...
: FliL is an inner membrane protein, occupying a position between the rotor and the stator of the bacterial flagellar motor. Its proximity to, and interactions with, the MS (membrane and supramembranous) ring, the switch complex and the stator proteins MotA/B suggests a role in recruitment and/or stabilization of the stator around the rotor, although the precise role of FliL in the flagellum remains to be established. In this study, recombinant C-terminal domain of Helicobacter pylori FliL (amin...
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Identification of natural ligands of chemoreceptors and structural studies aimed at elucidation of the molecular basis of the ligand specificity can be greatly facilitated by the production of milligram amounts of pure, folded ligand binding domains. Attempts to heterologously express periplasmic ligand binding domains of bacterial chemoreceptors in Escherichia coli (E. coli) often result in their targeting into inclusion bodies. Here, a method is presented for protein recovery from inclusion bo...
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#1Mayra A. MachucaH-Index: 5
#2Kevin S. JohnsonH-Index: 4
Last. Anna RoujeinikovaH-Index: 19
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#1Mohammad M. Rahman (DI: Discovery Institute)H-Index: 2
#2Daniel Phillip Germantsis (Monash University)H-Index: 1
Last. Anna RoujeinikovaH-Index: 19
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Helicobacter pylori infection can lead to the development of gastric and duodenal ulcers and gastric cancer. In recent years, the efficacy of the standard therapy has been falling, necessitating ongoing efforts to identify new drug targets. Due to their important role in chemotaxis and nutrient uptake, periplasmic binding proteins (PBPs) represent potential targets for new antimicrobial agents that have not yet been fully explored and exploited. The H. pylori PBP YckK is homologous to polar amin...
1 CitationsSource
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