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Kannan Ganapathy
University of Liverpool
63Publications
12H-index
362Citations
Publications 63
Newest
#1Christopher Ball (University of Liverpool)H-Index: 2
#2V. Felice (UNIBO: University of Bologna)H-Index: 2
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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AbstractRoutine diagnosis of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) is performed by collecting oropharyngeal swabs, followed by isolation and/or detection by molecular methods. ...
#1Christopher Ball (University of Liverpool)H-Index: 2
#2Anne Forrester (University of Liverpool)H-Index: 6
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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Abstract This study reports on the simultaneous administration of live NDV or aMPV subtype B vaccines alongside two live IBV (Massachusetts-H120 and 793B-CR88) vaccines in day-old maternal-antibody positive commercial broiler chicks. In the first experiment, chicks were divided into four groups; one unvaccinated and three groups vaccinated with live NDV VG/GA-Avinew, live H120 + CR88, or VG/GA-Avinew + H120 + CR88. In the second experiment, live aMPV subtype B vaccine was used in place of NDV. C...
#1Christopher Ball (University of Liverpool)H-Index: 2
#2Anne Forrester (University of Liverpool)H-Index: 6
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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Abstract Respiratory diseases continue to have a major impact on poultry health, welfare and productivity. However, little information is available on their current status in UK poultry flocks. We investigated the presence of four economically important respiratory pathogens in healthy or problematic flocks; infectious bronchitis virus (IBV), avian metapneumovirus (aMPV), Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae (Ms). Samples from 131 UK poultry flocks were received during the 12 mo...
#1Rajesh Chhabra (University of Liverpool)H-Index: 5
#2Christopher Ball (University of Liverpool)H-Index: 2
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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Abstract Avian infectious bronchitis virus (IBV) continues to cause serious economic losses in global chicken production. Concurrent circulation of both classic and variant IBVs have been identified in most parts of the world, raising major challenges to global prevention and control efforts. Therefore, immunopathogenesis, particularly early host responses, needs to be better understood for effective control of diseases caused by different strains of IBVs. We investigated differing immunopathoge...
#1Basim Manswr (University of Liverpool)H-Index: 1
#2Christopher Ball (University of Liverpool)H-Index: 2
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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ABSTRACTSequence variability in the S1 gene determines the genotype of infectious bronchitis virus (IBV) strains. A single RT-PCR assay was developed to amplify and sequence the full S1 gene for six classical and variant IBVs (M41, D274, 793B, IS/885/00, IS/1494/06 and Q1) enriched in allantoic fluid (AF) or the same AF inoculated onto Flinders Technology Association (FTA) cards. Representative strains from each genotype were grown in specific-pathogen-free eggs and RNA was extracted from AF. Fu...
#1Christopher Ball (University of Liverpool)H-Index: 2
#2Faez Awad (University of Liverpool)H-Index: 6
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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ABSTRACTAn investigation was undertaken of the extent of genetic variation occurring within infectious bronchitis virus (IBV) vaccine strains following vaccination of day-old broiler chicks. Chicks were divided into seven groups, with two groups receiving single Massachusetts (Mass) vaccinations while the other four were inoculated with combinations of different IBV serotypes; Mass, 793B, D274 and Arkansas (Ark). The remaining group was maintained as an unvaccinated control. Following vaccinatio...
#1Sally Hutton (University of Liverpool)H-Index: 2
#2Judy M. Bettridge (University of Liverpool)H-Index: 7
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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A survey was conducted into respiratory infectious diseases of poultry on a chicken breeder farm run by the Ethiopian Institute of Agricultural Research (EIAR), located in Debre Zeit, Ethiopia. Oropharyngeal swabs were collected from 117 randomly selected birds, and blood was taken from a subset of 73 of these birds. A combination of serological and molecular methods was used for detection of pathogens. For the first time in Ethiopia, we report the detection of variant infectious bronchitis viru...
#1Christopher Ball (University of Liverpool)H-Index: 2
#2Sarah Bennett (University of Liverpool)H-Index: 1
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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Despite regular co-vaccination of two different strains of live infectious bronchitis vaccine viruses, little is known about possible mutations in these viruses following vaccination. As an alternative to chicks, this study used an in vitro infection model to identify single-nucleotide polymorphisms (SNPs) within the part-S1 gene of two live infectious bronchitis virus vaccine strains (793B and Massachusetts) following single or dual inoculation onto tracheal organ cultures. Results indicate tha...
#1Christopher Ball (University of Liverpool)H-Index: 2
#2Anne Forrester (University of Liverpool)H-Index: 6
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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Poultry production is an important sector of agriculture in Sri Lanka; however, there is a lack of information regarding circulation of infectious bronchitis virus (IBV). RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and contained the genotype 793B (n = 13; 68.42 %), D274 (n = 4; 21.05 %) or Massachusetts (n = 2; 10.53 %). All three genotypes contained at least one strain with less than 99 % nucleotide...
#1Faez Awad (University of Liverpool)H-Index: 6
#2Rajesh Chhabra (University of Liverpool)H-Index: 5
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
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Pathogenesis of an IS/885/00-like (IS/885) strain of variant infectious bronchitis virus (IBV) was examined in one day old specific pathogen free (SPF) and commercial broiler chicks. Chicks were humanely euthanized at 3, 6, 9, 12, 15, 21 and 28 days post infection (dpi) for necropsy examination, and tissues were collected for histopathology and virus detection by reverse transcription polymerase chain reaction (RT-PCR). Respiratory clinical signs and gross lesions consisting of tracheal caseous ...
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