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Nadine Urban
University of Jena
ChemistryOxidative stressCaenorhabditis elegansBiochemistryCell biology
11Publications
4H-index
898Citations
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Publications 11
Newest
#1Karl Köhnlein (FSU: University of Jena)
#2Nadine Urban (FSU: University of Jena)H-Index: 4
Last. Lars-Oliver Klotz (FSU: University of Jena)H-Index: 49
view all 7 authors...
Source
#1Karl Köhnlein (FSU: University of Jena)H-Index: 1
#2Nadine Urban (FSU: University of Jena)H-Index: 4
Last. Lars-Oliver Klotz (FSU: University of Jena)H-Index: 49
view all 10 authors...
Abstract Human selenium binding protein 1 (SELENBP1) was originally identified as a protein binding selenium, most likely as selenite. SELENBP1 is associated with cellular redox and thiol homeostasis in several respects, including its established role as a methanethiol oxidase that is involved in degradation of a methionine catabolite, generating hydrogen sulfide (H2S) and hydrogen peroxide (H2O2). As both H2S and reactive oxygen species (such as H2O2) are major regulators of Caenorhabditis eleg...
5 CitationsSource
#1Huaize Liu (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 1
#2Jie Ding (MLU: Martin Luther University of Halle-Wittenberg)
Last. Thorsten Pfirrmann (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 10
view all 10 authors...
ABSTRACTThe AMP-activated protein kinase (AMPK) regulates cellular energy homeostasis by sensing the metabolic status of the cell. AMPK is regulated by phosphorylation and dephosphorylation as a re...
Source
#1Andriy Cherkas (FSU: University of Jena)H-Index: 7
#2Abdullah Saif Mondol (Leibniz Institute of Photonic Technology)H-Index: 2
Last. Iwan W. Schie (Leibniz Institute of Photonic Technology)H-Index: 14
view all 7 authors...
Caenorhabditis elegans is an animal model frequently used in research on the effects of metabolism on organismal aging. This comes with a requirement for methods to investigate metabolite content, turnover, and distribution. The aim of our study was to assess the use of a label-free approach to determine both content and distribution of glycogen, the storage form of glucose, in C. elegans. To this end, we grew C. elegans worms under three different dietary conditions for 24–48 h, representing st...
2 CitationsSource
#1Andrea Gille (FSU: University of Jena)H-Index: 1
#2Abdullah Turkistani (U of A: University of Alberta)H-Index: 1
Last. Lars-Oliver Klotz (FSU: University of Jena)H-Index: 49
view all 10 authors...
Abstract Diethyl maleate (DEM), a thiol-reactive α,β-unsaturated carbonyl compound, depletes glutathione (GSH) in exposed cells and was previously shown by us to elicit a stress response in Caenorhabditis elegans that, at lower concentrations, results in enhanced stress resistance and longer lifespan. This hormetic response was mediated through both the Nrf2 ortholog, SKN-1, and the forkhead box O (FOXO) family transcription factor DAF-16. As FOXO signaling is evolutionarily conserved, we analyz...
1 CitationsSource
#1Karl Köhnlein (FSU: University of Jena)
#2Nadine Urban (FSU: University of Jena)H-Index: 4
Last. Lars-Oliver Klotz (FSU: University of Jena)H-Index: 49
view all 4 authors...
Selenium-binding proteins do not contain selenium in the form of selenocysteine or selenomethionine but as inorganic selenium bound to the protein. The C. elegans genome encodes only one selenocysteine-containing selenoprotein, TrxR-1. However, at least two ORFs encode putative selenium-binding proteins, CeSELENBP1 and CeSELENBP2. These are 54% and 36% homologous to human selenium-binding protein-1 (SELENBP1), respectively, and both contain a cysteine residue hypothesized to bind selenite as in ...
Source
#1Nadine Urban (FSU: University of Jena)H-Index: 4
#2Dimitrios Tsitsipatis (FSU: University of Jena)H-Index: 2
Last. Lars-Oliver Klotz (FSU: University of Jena)H-Index: 49
view all 9 authors...
Abstract The redox environment in cells and organisms is set by low-molecular mass and protein-bound thiols, with glutathione (GSH) representing a major intracellular redox buffer. Subtle thiol oxidation elicits signal transduction processes and adaptive responses to cope with stressors, whereas highly oxidizing conditions may provoke cell death. We here tested how thiol depletion affects life span, stress resistance and stress signaling in the model organism Caenorhabditis elegans . Diethyl mal...
26 CitationsSource
#1Johannes MansfeldH-Index: 6
#2Nadine Urban (FSU: University of Jena)H-Index: 4
Last. Michael RistowH-Index: 54
view all 24 authors...
Ageing has been defined as a global decline in physiological function depending on both environmental and genetic factors. Here we identify gene transcripts that are similarly regulated during physiological ageing in nematodes, zebrafish and mice. We observe the strongest extension of lifespan when impairing expression of the branched-chain amino acid transferase-1 (bcat-1) gene in C. elegans, which leads to excessive levels of branched-chain amino acids (BCAAs). We further show that BCAAs reduc...
56 CitationsSource
#1Nadine Urban (FSU: University of Jena)H-Index: 4
#2Dimitrios Tsitsipatis (FSU: University of Jena)H-Index: 2
Last. Lars-Oliver Klotz (FSU: University of Jena)H-Index: 49
view all 6 authors...
Abstract Diethyl maleate (DEM) is a thiol-depleting agent frequently employed in cell culture analyses. Here, we investigated the effect of DEM exposure on insulin signaling at the level of FoxO transcription factor activity and its potential consequences for stress resistance and life span. Exposure of HepG2 human hepatoma cells to subcytotoxic concentrations of DEM resulted in nuclear accumulation of overexpressed EGFP-tagged FoxO1a. DEM-induced nuclear accumulation overrode insulin-induced nu...
Source
#1Tim J. Schulz (FSU: University of Jena)H-Index: 26
#2Kim Zarse (FSU: University of Jena)H-Index: 23
Last. Michael Ristow (FSU: University of Jena)H-Index: 54
view all 6 authors...
Summary Increasing cellular glucose uptake is a fundamental concept in treatment of type 2 diabetes, whereas nutritive calorie restriction increases life expectancy. We show here that increased glucose availability decreases Caenorhabditis elegans life span, while impaired glucose metabolism extends life expectancy by inducing mitochondrial respiration. The histone deacetylase Sir2.1 is found here to be dispensable for this phenotype, whereas disruption of aak-2 , a homolog of AMP-dependent kina...
771 CitationsSource
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