Xiao-En Xu
Fudan University
Publications 9
#1Shui-Ping Gao (Fudan University)H-Index: 6
#2He-Fen Sun (Fudan University)H-Index: 7
Last.Wei Jin (Fudan University)H-Index: 15
view all 7 authors...
TIM50 is an essential component of TIM23 complex and involved in protein translocating into the inner mitochondrial membrane. Here, we found that TIM50 was increased in breast cancer cells by SILAC. However, its biological functions and molecular mechanisms in breast cancer are poorly understood. To gain insight into the functions of TIM50 in breast cancer, we constructed two stably transfected cell lines and examined TIM50 expression in tissue samples. Our data showed that TIM50 expression was ...
7 CitationsSource
#1Shui-Ping GaoH-Index: 6
#2He-Fen SunH-Index: 7
Last.Wei JinH-Index: 15
view all 7 authors...
// Shui-Ping Gao 1,2,* , He-Fen Sun 1,2,* , Hong-Lin Jiang 1,2 , Liang-Dong Li 1,2 , Xin Hu 1 , Xiao-En Xu 1,2 and Wei Jin 1,2 1 Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Collaborative Innovation Center for Cancer Medicine, Fudan University Shanghai Cancer Center, Shanghai, China 2 Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China * These authors have contributed equally to this work Correspondence to: Wei Jin, email: // Keywords...
12 CitationsSource
#1Sun-Xia Chen (Fudan University)H-Index: 3
#2Xiao-En Xu (Fudan University)H-Index: 7
Last.FengLiu (Fudan University)H-Index: 17
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The tumor cell proliferation, migration and invasion were influenced by the interaction between the cancer cells and their microenvironment. In current study, we established two pairs of the primary fibroblast cultures from colorectal adenocarcinoma tissues and the normal counterparts and identified 227 proteins in the colonic fibroblast secretomes; half of these proteins were novel. The mass spectrometry data and analyzed results presented here provide novel insights into the molecular characte...
3 CitationsSource
#1Sun-Xia Chen (Fudan University)H-Index: 3
#2Xiao-En Xu (Fudan University)H-Index: 7
Last.FengLiu (Fudan University)H-Index: 17
view all 12 authors...
Abstract Stromal microenvironment influences tumor cell proliferation and migration. Fibroblasts represent the most abundant stromal constituents. Here, we established two pairs of normal fibroblast (NF) and cancer-associated fibroblast (CAF) cultures from colorectal adenocarcinoma tissues and the normal counterparts. The NFs and CAFs were stained positive for typical fibroblast markers and inhibited colon cancer (CC) cell proliferation in in vitro cocultures and in xenograft mouse models. The f...
20 CitationsSource
#1Ping Wei (Fudan University)H-Index: 2
#2Wei Zhang (Fudan University)H-Index: 1
Last.Qian Shi (Fudan University)H-Index: 9
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Glioma is the most common primary brain tumor, yet the high cost of diagnostic imaging has made early detection of asymptomatic glioma a formidable challenge. Thus, the development of a convenient, sensitive, and cost-effective diagnostic strategy, such as enzyme-linked immunosorbent assay (ELISA) based on glioma-specific and World Health Organization (WHO) grade-specific autoantibody serum markers, is necessary. To this end, a comparative proteomic analysis based on two-dimensional western blot...
16 CitationsSource
#1Liu-song Yang (Fudan University)H-Index: 1
#2Xiao-En Xu (Fudan University)H-Index: 7
Last.Qian Shi (Fudan University)H-Index: 1
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Abstract The oligodendroglioma (OG) type of glial cell tumors accounts for 2–5% of primary brain neoplasms and 4–15% of gliomas diagnosed worldwide. Allelic losses on 1p, or on 1p and 19q, correlate with chemotherapy response and good prognosis in OG patients; however, the underlying mechanisms are not yet clearly defined. Therefore, we utilized a quantitative proteomics strategy that combined 8-plex isobaric tags for relative and absolute quantitation (iTRAQ) labeling and two-dimensional liquid...
7 CitationsSource
#1Xiao-En Xu (Fudan University)H-Index: 7
#2Meng Qiao (Harvard University)H-Index: 7
Last.Qian Shi (Fudan University)H-Index: 9
view all 14 authors...
The proteins involved in breast cancer initiation and progression are still largely elusive. To gain insights into these processes, we conducted quantitative proteomic analyses with 21T series of breast cell lines, which include a normal, primary tumor and a metastatic tumor that were isolated from a single patient. Stable isotope labeling of amino acid in cell culture followed by LC-MS/MS analysis was performed and deregulated proteins were identified using statistical analysis. Gene ontology a...
46 CitationsSource
#1Meng Qiao (Brigham and Women's Hospital)H-Index: 7
#2Yaqi Wang (Fudan University)H-Index: 2
Last.Arthur B. Pardee (Brigham and Women's Hospital)H-Index: 87
view all 11 authors...
PHLPP1 and PHLPP2 phosphatases exert their tumor-suppressing functions by dephosphorylation and inactivation of Akt in several breast cancer and glioblastoma cells. However, Akt, or other known targets of PHLPPs that include PKC and ERK, may not fully elucidate the physiological role of the multifunctional phosphatases, especially their powerful apoptosis induction function. Here, we show that PHLPPs induce apoptosis in cancer cells independent of the known targets of PHLPPs. We identified Mst1 ...
80 CitationsSource
#1Xiao-En Xu (Fudan University)H-Index: 7
#2Jiang YhH-Index: 1
Last.Shi QH-Index: 1
view all 3 authors...
The application of proteomics techniques to tumor biological research promoted the emergence of Tumor Proteomics.Its major goal is to screen biomarkers for tumor detection,diagnosis,prognosis,as well as tumor treatment using proteomics technologies,such as two dimensional gel electrophoresis(2-DE),stable isotope labeling of amino acid in cell culture(SILAC) and matrix assisted laser desorption/ionization(MALDI).Three stages of tumor proteomics study,including expression proteomics,post-translati...
1 Citations