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Yigang Yu
South China University of Technology
ChemistryReal-time polymerase chain reactionBiochemistryBiologyMicrobiology
45Publications
9H-index
352Citations
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Publications 31
Newest
#1Ailian Zhou (SCUT: South China University of Technology)
#2Yifang Cao (SCUT: South China University of Technology)
Last. Xiaofeng Li (SCUT: South China University of Technology)H-Index: 14
view all 8 authors...
Abstract Cronobacter sakazakii is a common foodborne pathogen that can tolerate various stress conditions. Acidic environment is a common stress condition encountered by bacteria in food processing and gastrointestinal digestion, including both inorganic and organic acids. In order to elucidate the Acid Tolerance Response (ATR) of C. sakazakii, we performed high-throughput RNA-seq to compare gene expression under hydrochloric acid and citric acid stresses. In this study, 107 differentially expre...
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#1Yifang Cao (SCUT: South China University of Technology)
#2Ailian Zhou (SCUT: South China University of Technology)
Last. Xiaofeng Li (SCUT: South China University of Technology)H-Index: 14
view all 6 authors...
Abstract Response mechanism of Cronobacter sakazakii under stress of combination of citral and carvacrol was investigated by transcriptome sequencing. Twenty-five significantly differentially expressed genes were identified to be closely related to the ability of C. sakazakii tolerating the stress after analysis, mainly involving glycerol metabolism; citrate metabolism; formate metabolism; ribosome function and transmembrane transport system. Combination of carvacrol and citral could cause dissi...
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#1Yifang Cao (SCUT: South China University of Technology)
#2Donggen ZhouH-Index: 2
Last. Xiaofeng Li (SCUT: South China University of Technology)H-Index: 14
view all 8 authors...
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#1Yaru Li (SCUT: South China University of Technology)H-Index: 1
#2Donggen ZhouH-Index: 2
Last. Xiaofeng Li (SCUT: South China University of Technology)H-Index: 14
view all 6 authors...
Abstract The adaptability of Escherichia coli O157:H7 to different environmental stresses, including prolonged cold stress, poses a threat to food safety. In order to elucidate the prolonged cold response in E. coli O157:H7, genes related to prolonged coldresistance and their expression changes in E. coli O157:H7 ATCC35150 were screened and identified by analyzing their transcriptomedifferences between normal (37 °C) and low temperature storage (4 °C or −20 °C, 10 d), using RNA-sequencing. Accor...
3 CitationsSource
#1Shuangfang Hu (SCUT: South China University of Technology)H-Index: 4
#2Yigang Yu (SCUT: South China University of Technology)H-Index: 9
Last. Hui Wu (SCUT: South China University of Technology)H-Index: 14
view all 6 authors...
Abstract Salmonella is a major foodborne pathogen throughout the world and able to resist extreme acid stress such as stomach gastric juice after mild acid adaptation, which makes it a potential hazard in food industry. We conducted a comparative transcriptomic analysis by high-throughout RNA-seq to elucidate the Acid Tolerance Response (ATR) in Salmonella Enteritidis. Among these 554 differentially expressed genes (DEGs) identified in this study, the expression of 280 DEGs were up-regulated and...
3 CitationsSource
#1Xiaofeng Li (SCUT: South China University of Technology)H-Index: 14
#2Haixia Xu (SCUT: South China University of Technology)
Last. Xinglong Xiao (SCUT: South China University of Technology)H-Index: 9
view all 7 authors...
Acylation modification of phenol glycosides is currently of great interest due to the improved bioavailability and multiple functions. In this work, mono- or diesters of arbutin, an important phenol glycoside derivative, can be controllably synthesized by using whole-cell biocatalytic systems. Among fourteen microbial strains selected, Candida parapsilosis cells showed the best catalytic activity and high organic solvent tolerance. Compared with the best pure solvent tetrahydrofuran, the use of ...
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#1Shuangfang Hu (SCUT: South China University of Technology)H-Index: 4
#2Yigang Yu (SCUT: South China University of Technology)H-Index: 9
Last. Xinglong Xiao (SCUT: South China University of Technology)H-Index: 9
view all 3 authors...
Abstract Cronobacter is a group of opportunistic pathogen responsible for necrotizing enterocolitis, meningitis and septicaemia especially to infant and neonate, with high lethality ranging in 40%–80%. Some of them are able to survive in infant milk formula and still possesses capability of pathogenicity and virulence, biofilm formation, and high resistance to elevated osmotic pressure, low pH, heat, oxidation and desiccation. The genus Cronobacter consists of a diverse group of Gram-negative ba...
3 CitationsSource
#1Shuangfang Hu (SCUT: South China University of Technology)H-Index: 4
#2Yigang Yu (SCUT: South China University of Technology)H-Index: 9
Last. Hui Wu (SCUT: South China University of Technology)H-Index: 14
view all 6 authors...
A combination method of real-time PCR and high-resolution melting (HRM) analysis for the rapid detection and specific classification of six pathogenic Cronobacter species based on gene cgcA was developed. HRM profiles with distinct Tm (melting temperature) peaks were consistently obtained with each species, represented by a single peak ranging from 86.02 to 86.80 °C. The detection limit ranged from 0.1 to 1 pg per reaction. Desiccated Cronobacter sakazakii and Cronobacter muytjensii of 2 cfu/25 ...
1 CitationsSource
#1Shuangfang Hu (SCUT: South China University of Technology)H-Index: 4
#2Yigang Yu (SCUT: South China University of Technology)H-Index: 9
Last. Hui Wu (SCUT: South China University of Technology)H-Index: 14
view all 6 authors...
Abstract Cronobacter sakazakii is a foodborne pathogen throughout the world and survives extremely desiccation stress. However, the molecular basis involved in desiccation resistance of C. sakazakii is still unknown. In this study, the potential desiccation resistance factors of C. sakazakii ATCC 29544 were determined using iTRAQ-based quantitative proteomic analysis. A total of 2775 proteins were identified by iTRAQ, of which 233 showed a different protein expression between control group and d...
9 CitationsSource
#1Shuangfang Hu (SCUT: South China University of Technology)H-Index: 4
#2Xinglong Xiao (SCUT: South China University of Technology)H-Index: 9
Last. Hui Wu (SCUT: South China University of Technology)H-Index: 14
view all 6 authors...
Abstract ATR in EHEC O157:H7 and the cross-protection effects thus induced lead to the uncertainty of food safety. This study aimed to identify factors that are associated with the ATR in EHEC O157:H7 under stomach acidity using RNA-seq. In total, 223 DEGs in E. coli O157:H7 and 110 DEGs in E. coli after acid treatment were identified, including 118 upregulated and 105 downregulated in EHEC O157:H7, and 89 upregulated and 21 downregulated in E. coli ATCC 25922. According to our results, when fac...
4 CitationsSource
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