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Namil Lee
KAIST
12Publications
2H-index
23Citations
Publications 12
Newest
#1Yongjae Lee (KAIST)H-Index: 1
#2Namil Lee (KAIST)H-Index: 2
Last.Byung-Kwan Cho (KAIST)H-Index: 27
view all 8 authors...
Streptomyces lividans is an attractive host for production of heterologous proteins and secondary metabolites of other Streptomyces species. To fully harness the industrial potential of S. lividans, understanding its metabolism and genetic regulatory elements is essential. This study aimed to determine its transcription unit (TU) architecture and elucidate its diverse regulatory elements, including promoters, ribosome binding sites, 5-untranslated regions, and transcription terminators. Total 1...
Source
#1Hang Soo Cho (Yonsei University)H-Index: 1
#2Jin Chul Jo (Ewha Womans University)
Last.Yeo Joon Yoon (Ewha Womans University)H-Index: 2
view all 10 authors...
Genomic analysis of the clavulanic acid (CA)-high-producing Streptomyces clavuligerus strains, OL13 and OR, developed through random mutagenesis revealed a frameshift mutation in the cas1 gene-encoding clavaminate synthase 1. Overexpression of the intact cas1 in S. clavuligerus OR enhanced the CA titer by approximately 25%, producing ~ 4.95 g/L of CA, over the OR strain in the flask culture. Moreover, overexpression of the pathway-specific positive regulatory genes, ccaR and claR, in the OR stra...
Source
#1Soonkyu Hwang (KAIST)H-Index: 5
#2Namil Lee (KAIST)H-Index: 2
Last.Byung-Kwan Cho (KAIST)H-Index: 27
view all 8 authors...
Source
#1Namil Lee (KAIST)H-Index: 2
#2Soonkyu Hwang (KAIST)H-Index: 5
Last.Byung-Kwan ChoH-Index: 27
view all 6 authors...
2 CitationsSource
#1Jongoh Shin (KAIST)H-Index: 6
#2Namil Lee (KAIST)H-Index: 2
Last.Byung-Kwan Cho (KAIST)H-Index: 27
view all 4 authors...
1 CitationsSource
#1Namil LeeH-Index: 2
#2Jongoh ShinH-Index: 6
Last.Byung-Kwan ChoH-Index: 27
view all 6 authors...
Chinese hamster ovary (CHO) cells are the preferred host for the production of a wide array of biopharmaceuticals. Thus, efficient and rational CHO cell line engineering methods have been in high demand to improve quality and productivity. Here, we provide a novel genome engineering platform for increasing desirable phenotypes of CHO cells based upon the integrative protocol of high-throughput RNA sequencing and DNA-free RNA-guided Cas9 (CRISPR associated protein9) nuclease-based genome editing....
14 CitationsSource
#1Jongoh Shin (KAIST)H-Index: 6
#2Namil Lee (KAIST)H-Index: 2
Last.Byung-Kwan Cho (KAIST)H-Index: 27
view all 8 authors...
Increasing demand for recombinant therapeutic proteins has warranted the need for an efficient host cell to produce high-quality proteins, with a high yield. Chinese hamster ovary (CHO) cells appear to meet this demand, and their genetic tailoring will facilitate improvements in their productivity for recombinant proteins. Recent advances in programmable RNA-guided Cas9 nuclease (RGN) have facilitated CHO cell engineering via site-specific genome editing. One critical determinant for increasing ...
6 CitationsSource
#1Namil LeeH-Index: 2
#2Jinhyung ParkH-Index: 12
Last.Byung-Kwan ChoH-Index: 27
view all 6 authors...
#2Namil LeeH-Index: 2
Last.Byung-Kwan ChoH-Index: 27
view all 4 authors...
#1Jongoh ShinH-Index: 6
#2Namil LeeH-Index: 2
Last.Byung-Kwan ChoH-Index: 27
view all 6 authors...
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