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Yu. G. Kuznetsov
University of California, Irvine
30Publications
19H-index
1,149Citations
Publications 30
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#1Yu. G. Kuznetsov (UCI: University of California, Irvine)H-Index: 19
#2Pavel UlbrichH-Index: 18
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
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Abstract Particles of ΔProCANC, a fusion of capsid (CA) and nucleocapsid (NC) protein of Mason–Pfizer monkey virus (M-PMV), which lacks the amino terminal proline, were reassembled in vitro and visualized by atomic force microscopy (AFM). The particles, of 83–84 nm diameter, exhibited ordered domains based on trigonal arrays of prominent rings with center to center distances of 8.7 nm. Imperfect closure of the lattice on the spherical surface was affected by formation of discontinuities. The lat...
#1Yu. G. Kuznetsov (UCI: University of California, Irvine)H-Index: 19
#2Audrey Low (UCI: University of California, Irvine)H-Index: 6
Last.W. E. Robinson (UCI: University of California, Irvine)H-Index: 2
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Retroviruses are membrane-enveloped, RNA-containing viruses that produce a wide range of threatening diseases in higher animals. Among these are human immunodeficiency virus (HIV), which produces acquired immune deficiency syndrome (AIDS) in humans, and murine leukemia virus (MuLV), which produces leukemias in rodents. We have obtained the first atomic force microscopy (AFM) images of these two retroviruses, both isolated from culture media and emerging from infected cell surfaces. The HIV virio...
#1Yu. G. Kuznetsov (UCI: University of California, Irvine)H-Index: 19
#2Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
Abstract Turnip Yellow Mosaic Virus (TYMV) was subjected to a variety of procedures which disrupted the protein capsids and produced exposure of the ssRNA genome. The results of the treatments were visualized by atomic force microscopy (AFM). Both in situ and ex situ freeze–thawing produced RNA emission, though at low efficiency. The RNA lost from such particles was evident, in some cases in the process of exiting the virions. More severe disruption of TYMV and extrusion of intact RNA onto the s...
#1Yu. G. Kuznetsov (UCI: University of California, Irvine)H-Index: 19
#2Debora L. Makino (UCI: University of California, Irvine)H-Index: 5
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
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Virus crystals can incorporate a wide range of unusual impurities, not possible for conventional crystals, or even most protein crystals because of the large size of their constituent particles. These impurities include anomalous virions, satellite viruses and biological fibers. Examples of several of these unusual impurities are presented here, along with some of the consequences for the crystal lattices. The high solvent content, the forgiving character of the lattice and the plasticity of the...
#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 13
#2Yu. G. Kuznetsov (UCI: University of California, Irvine)H-Index: 19
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
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A genetically engineered humanized CH2-domain-deleted monoclonal antibody lacking any interchain-hinge disulfide bonds has been crystallized in the presence of detergent in a form suitable for X-ray diffraction analysis. The crystals were grown from 4 M formate along with Triton X-100 and had P21212 space-group symmetry, with unit-cell parameters a = 83, b = 224, c = 167 A. The crystals diffract to beyond 2.8 A resolution. A disordered crystal form of larger size and more attractive habit was al...
#1Yu. G. Kuznetsov (UCI: University of California, Irvine)H-Index: 19
#2James R. Gurnon (NU: University of Nebraska–Lincoln)H-Index: 21
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
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Abstract A virus PBCV-1, which infects certain fresh water algae and has been shown by transmission and cryo-electron microscopy to exist as a triskaidecahedron, was imaged using atomic force microscopy (AFM). From AFM the particles have diameters of about 190 nm and the overall structure is in all important respects consistent with existing models. The surface lattice of the virion is composed of trimeric capsid proteins distributed according to p3 symmetry to create a honeycomb arrangement of ...
#1Yu. G. KuznetsovH-Index: 19
#2J. G. VictoriaH-Index: 2
Last.Alex McPhersonH-Index: 9
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Isolated human immunodeficiency virus (HIV) and HIV-infected human lymphocytes in culture have been imaged for the first time by atomic force microscopy (AFM). Purified virus particles spread on glass substrates are roughly spherical, reasonably uniform, though pleomorphic in appearance, and have diameters of about 120 nm. Similar particles are also seen on infected cell surfaces, but morphologies and sizes are considerably more varied, possibly a reflection of the budding process. The surfaces ...
#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
#2Yu. G. Kuznetsov (UCI: University of California, Irvine)H-Index: 19
Last.Marco Plomp (UCI: University of California, Irvine)H-Index: 15
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Direct visualization of macromolecular crystal growth using atomic force microscopy (AFM) has provided a powerful tool in the delineation of mechanisms and the kinetics of the growth process. It has further allowed us to evaluate the wide variety of impurities that are incorporated into crystals of proteins, nucleic acids, and viruses. We can, using AFM, image the defects and imperfections that afflict these crystals, the impurity layers that poison their surfaces, and the consequences of variou...
#1Alexander J. Malkin (UCI: University of California, Irvine)H-Index: 22
#2Yu. G. Kuznetsov (UCI: University of California, Irvine)H-Index: 19
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
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Abstract In situ atomic force microscopy (AFM) was used to investigate surface evolution during the growth of single crystals of turnip yellow mosaic virus (TYMV), cucumber mosaic virus (CMV) and glucose isomerase. Growth of these crystals proceeded by two-dimensional (2D) nucleation. For glucose isomerase, from supersaturation dependencies of tangential step rates and critical step length, the kinetic coefficients of the steps and the surface free energy of the step edge were calculated for dif...
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