Kohichi Tanaka
Tokyo Medical and Dental University
Publications 180
#1Mizuki Uchida (Meijo University)H-Index: 1
#2Hirotake Hida (Meijo University)H-Index: 4
Last.Norio Ozaki (Nagoya University)H-Index: 56
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Abstract Alterations of the glutamatergic system components, including N -methyl- d -aspartate (NMDA) receptors are relevant to the pathophysiology of schizophrenia. Repeated phencyclidine (PCP) administration induces several schizophrenia-like psychobehavioral abnormalities and decreases extracellular glutamate levels, which are associated with increased levels of glial glutamate and aspartate transporter (GLAST) in the prefrontal cortex (PFC) of mice. In the present study, we investigated the ...
#1Shota Nakade (Hiroshima University)H-Index: 6
#2Keiji Mochida (Hiroshima University)H-Index: 2
Last.Takashi Yamamoto (Hiroshima University)H-Index: 43
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Selective genome editing such as gene knock-in has recently been achieved by administration of chemical enhancer or inhibitor of particular DNA double-strand break (DSB) repair pathways, as well as overexpression of pathway-specific genes. In this study, we attempt to enhance the efficiency further to secure robust gene knock-ins, by using the local accumulation of DSB repair molecules (LoAD) system. We identify CtIP as a strong enhancer of microhomology-mediated end-joining (MMEJ) repair by gen...
#1Zhuoyang Zhao (Tokyo Medical and Dental University)H-Index: 1
#2Yuichi Hiraoka (Tokyo Medical and Dental University)H-Index: 3
Last.Kohichi Tanaka (Tokyo Medical and Dental University)H-Index: 51
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#1Emma M. Perkins (Edin.: University of Edinburgh)H-Index: 9
#2Yvonne L. Clarkson (Edin.: University of Edinburgh)H-Index: 6
Last.Mandy Jackson (Edin.: University of Edinburgh)H-Index: 13
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#1Bettina Reichenbach (KI: Karolinska Institutet)H-Index: 1
#2Johanna Classon (KI: Karolinska Institutet)H-Index: 2
Last.Christian Göritz (KI: Karolinska Institutet)H-Index: 16
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Abstract Tissues contain distinct stem cell niches, but whether cell turnover is coordinated between niches during growth is unknown. Here, we report that in mouse skin, hair growth is accompanied by sebaceous gland and interfollicular epidermis expansion. During hair growth, cells in the bulge and outer root sheath temporarily upregulate the glutamate transporter SLC1A3, and the number of SLC1A3 + basal cells in interfollicular epidermis and sebaceous gland increases. Fate mapping of SLC1A3 + c...
#1Junya Sugimoto (Tokyo Medical and Dental University)H-Index: 1
#2Moeko Tanaka (Tokyo Medical and Dental University)H-Index: 1
Last.Kohichi Tanaka (Tokyo Medical and Dental University)H-Index: 51
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#1Kaori Sugiyama (Tokyo Medical and Dental University)H-Index: 2
#2Kohichi Tanaka (Tokyo Medical and Dental University)H-Index: 51
Abstract Amyotrophic lateral sclerosis (ALS) is a chronic neurodegenerative disorder characterized by the selective loss of motor neurons. The precise mechanisms that cause the selective death of motor neurons remain unclear, but a growing body of evidence suggests that glutamate-mediated excitotoxicity has been considered to play an important role in the mechanisms of motor neuron degeneration in ALS. Reductions in glutamate transporter GLT1 have been reported in animal models of ALS and the mo...
#1Kaori Sugiyama (Tokyo Medical and Dental University)H-Index: 2
#2Tomomi Aida (Tokyo Medical and Dental University)H-Index: 11
Last.Kohichi Tanaka (Tokyo Medical and Dental University)H-Index: 51
view all 6 authors...
Glutamate-mediated excitotoxicity induces neuronal death by altering various intracellular signaling pathways and is implicated as a common pathogenic pathway in many neurodegenerative diseases. In the case of motor neuron disease, there is significant evidence to suggest that overactivation of AMPA receptors due to deficiencies in the expression and function of glial glutamate transporter GLT1 and GLAST plays an important role in the mechanisms of neuronal death. However, a causal role for glia...
#1Taisuke Miyazaki (Hokkaido University)H-Index: 26
#2Miwako Yamasaki (Hokkaido University)H-Index: 27
Last.Masahiko Watanabe (Hokkaido University)H-Index: 101
view all 9 authors...
Astrocytes regulate synaptic transmission through controlling neurotransmitter concentrations around synapses. Little is known, however, about their roles in neural circuit development. Here we report that Bergmann glia (BG), specialized cerebellar astrocytes that thoroughly enwrap Purkinje cells (PCs), are essential for synaptic organization in PCs through the action of the l-glutamate/l-aspartate transporter (GLAST). In GLAST-knockout mice, dendritic innervation by the main ascending climbing ...