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Yanping Huang
Children's Hospital of Philadelphia
Human leukocyte antigenImmunologyTypingMedicineBiology
11Publications
2H-index
18Citations
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Publications 11
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#1Yanping Huang (Children's Hospital of Philadelphia)H-Index: 2
#2Anh Dinh (Children's Hospital of Philadelphia)H-Index: 2
Last. Dimitri S. Monos (UPenn: University of Pennsylvania)H-Index: 34
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2 CitationsSource
#1Joseph H. Oved (Children's Hospital of Philadelphia)
#2Natasha Stanley (Children's Hospital of Philadelphia)H-Index: 3
Last. Timothy S. Olson (Children's Hospital of Philadelphia)H-Index: 11
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Source
#1Joseph H. Oved (Children's Hospital of Philadelphia)
#2Yongping Wang (UPenn: University of Pennsylvania)H-Index: 12
Last. Timothy S. Olson (UPenn: University of Pennsylvania)H-Index: 11
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Source
#1Larissa Slavich (Children's Hospital of Philadelphia)H-Index: 2
#2Yanping Huang (Children's Hospital of Philadelphia)H-Index: 2
Last. Dimitri S. Monos (Children's Hospital of Philadelphia)H-Index: 34
view all 4 authors...
The monitoring of chimerism is a standard procedure to help assess engraftment and achievement of full donor lymphoid cells after allogeneic stem cell transplantation. T cell chimerism analysis is essential since T cells support the engraftment of hematopoietic stem cells and progenitor cells. TCR αβ T cells provide anti-tumor activity but can also cause graft vs. host disease (GVHD). In contrast, TCR γδ T cells have been found not to be associated with GVHD. Here we report a case in which resid...
Source
#1Allison Gasiewski (Children's Hospital of Philadelphia)H-Index: 2
#2Ryan Morlen (Children's Hospital of Philadelphia)H-Index: 1
Last. Dimitri S. Monos (UPenn: University of Pennsylvania)H-Index: 34
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Aim The aim of this method is to obtain clear, fast, and accurate virtual crossmatch results which enhance communication with our clinical programs. Methods Virtual crossmatching is essential for heart and lung transplantation due to the need for short ischemia times and is increasingly used in kidney transplantation to facilitate organ allocation. There are currently no reliable tools for performing a virtual crossmatch. Our previous method was to manually assess the patient’s antibody profile ...
Source
#1Yanping Huang (Children's Hospital of Philadelphia)H-Index: 2
#2Timothy S. Olson (Children's Hospital of Philadelphia)H-Index: 11
Last. Dimitri S. Monos (UPenn: University of Pennsylvania)H-Index: 34
view all 3 authors...
MIRAGE (myelodysplasia, infection, restriction of growth, adrenal hypoplasia, genital phenotypes and enteropathy) syndrome is a recently described multisystem disorder that is related to gain of function of the growth repressor SAMD9. Due to the dominant suppressive effect of SAMD9 mutants, MIRAGE syndrome is associated with high mortality. A new publication this year suggests bone marrow transplantation (BMT) may be necessary for survival. Here we report a case of a patient who developed unexpe...
Source
#1Manish J. GandhiH-Index: 21
#2Deborah FerriolaH-Index: 10
Last. Dimitri S. Monos (Children's Hospital of Philadelphia)H-Index: 34
view all 5 authors...
Context.— Numerous feasibility studies to type human leukocyte antigens (HLAs) by next-generation sequencing (NGS) have led to the development of vendor-supported kits for HLA typing by NGS. Some clinical laboratories have introduced HLA-NGS, and many are investigating the introduction. Standards from accrediting agencies form the regulatory framework for introducing this test into clinical laboratories. Objectives.— To provide an assessment of metrics and considerations relevant to the successf...
15 CitationsSource
#1Yanping Huang (Children's Hospital of Philadelphia)H-Index: 2
#2Allison Gasiewski (Children's Hospital of Philadelphia)H-Index: 2
Last. Dimitri S. Monos (Children's Hospital of Philadelphia)H-Index: 34
view all 6 authors...
NGS based HLA typing is useful for the bone marrow transplantation, but its utility for solid organ transplantation (SOT) is unclear. Our lab has been using NGS based HLA typing as the primary methodology to support all transplant programs. Here, we present three cases to show the impact of this technology on SOT. Case 1: Relevance to living donor selection. A 15-year old girl required a kidney transplant due to focal segmental glomerulosclerosis. Prior to transplant, anti-HLA DQ2 antibody was d...
Source
#1Jamie L. Duke (Children's Hospital of Philadelphia)H-Index: 6
#2Daria V. Babushok (Children's Hospital of Philadelphia)H-Index: 11
Last. Dimitri S. Monos (UPenn: University of Pennsylvania)H-Index: 34
view all 7 authors...
Aim Next-generation sequencing (NGS) has assisted in the full characterization of HLA alleles, allowing in-depth studies of how these genes can play a direct role in the pathophysiology of many diseases outside of the context of transplantation. Here, we sought to determine the degree to which NGS can be used to characterize clonal somatic mutations acquired in HLA genes during the progression of aplastic anemia. Methods Omixon’s Holotype Kit was used to sequence HLA-A, B, C and DRB1 on the Illu...
Source
#1Yanping Huang (Children's Hospital of Philadelphia)H-Index: 2
Last. Dimitri S. Monos (UPenn: University of Pennsylvania)H-Index: 34
view all 5 authors...
Aim Automate amplification and library preparation using the NGS-based Holotype HLA kit for 7 loci (A, B, C, DRB1, DPB1, DQA1 and DQB1). Methods Amplification and library preparation as dictated by the Omixon Holotype kit were automated on 2 Hamilton STARlet systems. PCR setup is performed on a dedicated pre-PCR STARlet with no peripheral equipment. A user interface allows input of sample ID, DNA concentration, and loci to be amplified from a .csv file. Pre-PCR programs include master mix prepar...
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