Bart O. Roep
City of Hope National Medical Center
270Publications
58H-index
10.8kCitations
Publications 270
Newest
Published on Mar 1, 2019in Nature 41.58
Kenichiro Furuyama2
Estimated H-index: 2
(University of Geneva),
Simona Chera18
Estimated H-index: 18
(University of Bergen)
+ 14 AuthorsThierry Berney38
Estimated H-index: 38
(University of Geneva)
Cell-identity switches, in which terminally differentiated cells are converted into different cell types when stressed, represent a widespread regenerative strategy in animals, yet they are poorly documented in mammals. In mice, some glucagon-producing pancreatic α-cells and somatostatin-producing δ-cells become insulin-expressing cells after the ablation of insulin-secreting β-cells, thus promoting diabetes recovery. Whether human islets also display this plasticity, especially in diabetic cond...
4 Citations Source Cite
Published on Jan 1, 2019in The Lancet Diabetes & Endocrinology 19.31
Bart O. Roep58
Estimated H-index: 58
(City of Hope National Medical Center),
Daniel C S Wheeler1
Estimated H-index: 1
(King's College London),
Mark Peakman51
Estimated H-index: 51
(HealthPartners)
Summary Precision medicine has emerged as a mantra for therapeutic approaches to complex diseases. The defining concept relies on a detailed insight into disease pathogenesis and therapeutic mechanism. Although the type 1 diabetes field has gained new insights into disease endotypes and indications of efficacy for several therapies, none of these is yet licensed, partly because of immune suppressive side-effects beyond control of islet autoimmunity. New strategies designed to regulate the immune...
2 Citations Source Cite
An-Sofie Vanherwegen5
Estimated H-index: 5
(Katholieke Universiteit Leuven),
Guy Eelen25
Estimated H-index: 25
(Katholieke Universiteit Leuven)
+ 8 AuthorsChantal Mathieu77
Estimated H-index: 77
(Katholieke Universiteit Leuven)
Abstract Tolerogenic dendritic cells (tolDCs) instruct regulatory T cells (Tregs) to dampen autoimmunity. Active vitamin D 3 (1α,25-dihydroxyvitamin D 3 ; 1α,25(OH) 2 D 3 ) imprints human monocyte-derived DCs with tolerogenic properties by reprogramming their glucose metabolism. Here we identify the glycolytic enzyme 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4) as a critical checkpoint and direct transcriptional target of 1α,25(OH) 2 D 3 in determining the tolDC profile. Using ...
2 Citations Source Cite
Published on Jan 1, 2019in The Lancet Diabetes & Endocrinology 19.31
Mark A. Atkinson82
Estimated H-index: 82
(University of Florida),
Bart O. Roep58
Estimated H-index: 58
(City of Hope National Medical Center)
+ 2 AuthorsMark Peakman51
Estimated H-index: 51
(HealthPartners)
Summary With the conceptual advance about four decades ago that type 1 diabetes represents an autoimmune disease, hope arose that immune-based therapies would soon emerge to prevent and reverse the disorder. However, despite dozens of clinical trials seeking to achieve these goals, the promise remains unfulfilled, at least in a pragmatic form. With the benefit of hindsight, several important reasons are likely to account for this disappointing outcome, including failure to appreciate disease het...
1 Citations Source Cite
Published on Apr 1, 2019in Diabetes 7.27
Menno van Lummel8
Estimated H-index: 8
(Leiden University Medical Center),
David T.P. Buis (Leiden University Medical Center)+ 7 AuthorsBart O. Roep58
Estimated H-index: 58
(City of Hope National Medical Center)
The heterozygous DQ2/8 (DQA1*05:01-DQB1*02:01/DQA1*03:01-DQB1*03:02) genotype confers the highest risk in type 1 diabetes (T1D), whereas the DQ6/8 (DQA1*02:01-DQB1*06:02/DQA1*03:01-DQB1*03:02) genotype is protective. The mechanism of dominant protection by DQ6 (DQB1*06:02) is unknown. We tested the hypothesis that DQ6 interferes with peptide binding to DQ8 by competition for islet epitope (“epitope stealing”) by analysis of the islet ligandome presented by HLA-DQ6/8 and -DQ8/8 on dendritic cells...
Source Cite
Published on Mar 1, 2018in Diabetologia 6.02
Eddie A. James29
Estimated H-index: 29
(Benaroya Research Institute),
Joana R. F. Abreu8
Estimated H-index: 8
(Leiden University)
+ 16 AuthorsAimon K. Alkanani8
Estimated H-index: 8
(University of Colorado Denver)
Aims/hypothesis Validated biomarkers are needed to monitor the effects of immune intervention in individuals with type 1 diabetes. Despite their importance, few options exist for monitoring antigen-specific T cells. Previous reports described a combinatorial approach that enables the simultaneous detection and quantification of multiple islet-specific CD8+ T cell populations. Here, we set out to evaluate the performance of a combinatorial HLA-A2 multimer assay in a multi-centre setting.
2 Citations Source Cite
Published on Jul 1, 2018in Diabetologia 6.02
Laurits J. Holm1
Estimated H-index: 1
,
Lars Krogvold10
Estimated H-index: 10
(University of Oslo)
+ 12 AuthorsBobby P. C. Koeleman4
Estimated H-index: 4
(University Medical Center)
Aims/hypothesis Sphingolipids play important roles in beta cell physiology, by regulating proinsulin folding and insulin secretion and in controlling apoptosis, as studied in animal models and cell cultures. Here we investigate whether sphingolipid metabolism may contribute to the pathogenesis of human type 1 diabetes and whether increasing the levels of the sphingolipid sulfatide would prevent models of diabetes in NOD mice.
4 Citations Source Cite
Published on Aug 1, 2018in Transplantation 3.96
Jon S. Odorico38
Estimated H-index: 38
(University of Wisconsin-Madison),
James F. Markmann51
Estimated H-index: 51
(Harvard University)
+ 33 AuthorsLuhan Yang1
Estimated H-index: 1
Beta cell replacement has the potential to restore euglycemia in patients with insulin dependent diabetes. While great progress has been made in establishing allogeneic islet transplantation from deceased donors as the standard of care for those with the most labile diabetes, it is also clear that the deceased donor organ supply cannot possibly treat all those who could benefit from restoration of a normal beta cell mass, especially if immunosuppression were not required. Against this background...
2 Citations Source Cite
Published on Jan 1, 2018in Frontiers in Immunology 5.51
Kayleigh M. van Megen1
Estimated H-index: 1
(Beckman Research Institute),
Ernst-Jan T. van ’t Wout (Beckman Research Institute)+ 1 AuthorsBart O. Roep58
Estimated H-index: 58
(Beckman Research Institute)
Source Cite
Published on Aug 8, 2018in PLOS ONE 2.77
Sandra Laban16
Estimated H-index: 16
,
Jessica S. Suwandi1
Estimated H-index: 1
+ 7 AuthorsBart O. Roep58
Estimated H-index: 58
Auto-reactive CD8 T-cells play an important role in the destruction of pancreatic β-cells resulting in type 1 diabetes (T1D). However, the phenotype of these auto-reactive cytolytic CD8 T-cells has not yet been extensively described. We used high-dimensional mass cytometry to phenotype autoantigen- (pre-proinsulin), neoantigen- (insulin-DRIP) and virus-(cytomegalovirus) reactive CD8 T-cells in peripheral blood mononuclear cells (PBMCs) of T1D patients. A panel of 33 monoclonal antibodies was des...
Source Cite
12345678910