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Irisappan Ganesh
Pohang University of Science and Technology
Two-component regulatory systemGreen fluorescent proteinMolecular biologyEscherichia coliBiochemistry
17Publications
8H-index
167Citations
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Publications 18
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#1Irisappan Ganesh (POSTECH: Pohang University of Science and Technology)
#1Irisappan Ganesh (POSTECH: Pohang University of Science and Technology)H-Index: 8
Last. Jeong Wook Lee (POSTECH: Pohang University of Science and Technology)H-Index: 1
view all 3 authors...
Gas molecules are ubiquitous in the environment and are used as nutrient and energy sources for living organisms. Many organisms, therefore, have developed gas-sensing systems to respond efficiently to changes in the atmospheric environment. In microorganisms and plants, two-component systems (TCSs) and transcription factors (TFs) are two primary mechanisms to sense gas molecules. In this review, we discuss gas-sensing transcriptional regulators, TCSs and TFs, focusing on protein structures, mec...
1 CitationsSource
#1Vidhya SelvamaniH-Index: 3
#2Irisappan GaneshH-Index: 8
Last. Soon Ho HongH-Index: 18
view all 5 authors...
Source
#1Irisappan Ganesh (UOU: University of Ulsan)H-Index: 8
#2Tae Wan Kim (Chonnam National University)H-Index: 5
Last. Soon Ho Hong (UOU: University of Ulsan)H-Index: 18
view all 5 authors...
The Two-component Regulatory System (TCS) is the primary mode that bacteria use to continuously sense the environment. A TCS is comprised of a periplasmic sensor Histidine kinase (HK) domain and a cytoplasmic Response regulator (RR) domain. The HK domain phosphorylates the RR domain to activate the effector gene expression. Utilizing a rational approach, the sensor HK was genetically engineered in Escherichia coli to create chimeric HK, by a rewiring or domain swapping strategy. Apart from the w...
Source
: Gamma-aminobutyric acid is a precursor of nylon-4, which is a promising heat-resistant biopolymer. GABA can be produced from the decarboxylation of glutamate by glutamate decarboxylase. In this study, a synthetic scaffold complex strategy was employed involving the Neurospora crassa glutamate decarboxylase (GadB) and Escherichia coli GABA antiporter (GadC) to improve GABA production. To construct the complex, the SH3 domain was attached to the N. crassa GadB, and the SH3 ligand was attached to...
2 CitationsSource
: Escherichia coli was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of ompC and the fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of...
4 CitationsSource
#1Vidhya Selvamani (UOU: University of Ulsan)H-Index: 3
#2Irisappan Ganesh (UOU: University of Ulsan)H-Index: 8
Last. Soon Ho Hong (UOU: University of Ulsan)H-Index: 18
view all 5 authors...
Escherichia coli does not have the methanol sensing apparatus, was engineered to sense methanol by employing chimeric two-component system (TCS) strategy. A chimeric FlhS/EnvZ (FlhSZ) chimeric histidine kinase (HK) was constructed by fusing the sensing domain of Paracoccus denitrificans FlhS with the catalytic domain of E. coli EnvZ. The constructed chimeric TCS FlhSZ/OmpR could sense methanol by the expression of ompC and gfp gene regulated by ompC promoter. Real-time quantitative PCR analysis ...
6 CitationsSource
#1Irisappan Ganesh (SKKU: Sungkyunkwan University)H-Index: 8
#2Buu Minh Tran (Gachon University)H-Index: 1
Last. Sungsu Park (SKKU: Sungkyunkwan University)H-Index: 37
view all 7 authors...
There is growing interest in rapid microbial pre-concentration methods to lower the detection limit of bacterial pathogens of low abundance in samples. Here, we report an integrated microfluidic PCR system that enables bacterial cells of interest in samples to be concentrated prior to PCR. It consists of two major compartments: a preconcentration chamber for the immunomagnetic separation of bacterial cells, and a PCR chamber for the DNA amplification of the concentrated cells. We demonstrate the...
14 CitationsSource
#1Irisappan Ganesh (UOU: University of Ulsan)H-Index: 8
#2Murali kannan Maruthamuthu (UOU: University of Ulsan)H-Index: 5
Last. Soon Ho Hong (UOU: University of Ulsan)H-Index: 18
view all 3 authors...
Previous studies constructed a chimeric MalKZ two-component system to sense environmental malate. In this study, we used a positive feedback loop to accelerate and amplify the output signal indicating malate concentration. The positive feedback loop was constructed by cloning ompR gene, which encodes ompC and induces OmpR protein; ompC promoter was used to control the process. The transcriptional expression profile showed that the expression level of ompC gene increased about two-fold after the ...
Source
#1Irisappan Ganesh (UOU: University of Ulsan)H-Index: 8
#2Murali kannan Maruthamuthu (UOU: University of Ulsan)H-Index: 5
Last. Soon Ho Hong (UOU: University of Ulsan)H-Index: 18
view all 4 authors...
A positive feedback loop was introduced to modify the dynamic behavior of fumarate sensing DcuSZ chimera TCS. To construct the positive feedback loop, the ompR gene was cloned downstream of the ompC promoter. The ompC promoter induced the expression of OmpR, which in turn induced the expression of the ompC promoter. Through the introduction of this positive feedback loop, the transcriptional expression levels of ompC increased 2.6-fold. When GFP was used as a reporter protein, a 64% increase in ...
Source
#1Murali kannan Maruthamuthu (UOU: University of Ulsan)H-Index: 5
#2Saravanan Prabhu Nadarajan (Konkuk University)H-Index: 11
Last. Soon Ho Hong (UOU: University of Ulsan)H-Index: 18
view all 7 authors...
For the construction of an efficient copper waste treatment system, a cell surface display strategy was employed. The copper adsorption ability of recombinant bacterial strains displaying three different copper binding peptides were evaluated in LB Luria–Bertani medium (LB), artificial wastewater, and copper phthalocyanine containing textile dye industry wastewater samples. Structural characteristics of the three peptides were also analyzed by similarity-based structure modeling. The best bindin...
6 CitationsSource
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