Lara S Chang
ProteasesPyrococcus furiosusThermotoga maritimaBiochemistryBiology
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Publications 4
#1P.M. Hicks (Cargill)
#2Lara S ChangH-Index: 3
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#1Donald E. WardH-Index: 11
#2Keith R. ShockleyH-Index: 14
Last. Robert M. KellyH-Index: 53
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Proteases are found in every cell, where they recognize and break down unneeded or abnormal polypeptides or peptide-based nutrients within or outside the cell. Genome sequence data can be used to compare proteolytic enzyme inventories of different organisms as they relate to physiological needs for protein modification and hydrolysis. In this review, we exploit genome sequence data to compare hyperthermophilic microorganisms from the euryarchaeotal genus Pyrococcus, the crenarchaeote Sulfolobus ...
37 CitationsSource
#1Paula M HicksH-Index: 4
#2Lara S ChangH-Index: 3
Last. Robert M. KellyH-Index: 53
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Publisher Summary Thermotoga maritima is an anaerobic heterotroph belonging to the bacterial order Thermotogales that grows optimally at 80° by fermentation of carbohydrates and proteins, including starch, glucose, galactose, glycogen, and yeast extract. The bacterium is also able to reduce thiosulfate to sulfide, with an improved growth rate. Although the microorganism is a facultative sulfur reducer, the reduction of sulfur does not provide an energetic boost as seen by the lack of effect on g...
4 CitationsSource
#1Lara S ChangH-Index: 3
#2Paula M HicksH-Index: 4
Last. Robert M. KellyH-Index: 53
view all 3 authors...
Publisher Summary Pyrococcus furiosus is a hyperthermophilic archaeon from the order Thermococcales capable of growth on a variety of proteinaceous and carbohydrate-containing substrates. Analysis of gelatin-containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) gels indicates that at least 11 endoproteinases are active in the cell extracts of this organism 2'3 and the following proteases have been characterized: protease I (PfpI), pyrolysin, proteasome, prolyl oligopepti...
9 CitationsSource