Weili Yang
Jinan University
Publications 12
#1Peng Yin (JNU: Jinan University)H-Index: 1
#2Xiangyu Guo (JNU: Jinan University)H-Index: 3
Last.Xiao-Jiang Li (JNU: Jinan University)H-Index: 1
view all 10 authors...
The cytoplasmic accumulation of the nuclear TAR DNA-binding protein 43 (TDP-43) is a pathologic hallmark in amyotrophic lateral sclerosis, frontotemporal lobar degeneration, and other neurological disorders. However, most transgenic TDP-43 rodent models show predominant nuclear distribution of TDP-43 in the brain. By expressing mutant TDP-43 (M337V) in the brains of rhesus monkeys and mice, we verified that mutant TDP-43 is distributed in the cytoplasm of the monkey brain and that the majority o...
3 CitationsSource
#1Weili Yang (JNU: Jinan University)H-Index: 9
#2Yunbo Liu (Peking Union Medical College)H-Index: 2
Last.Xiao-Jiang Li (Emory University)H-Index: 62
view all 15 authors...
6 CitationsSource
#1Sen Yan (JNU: Jinan University)H-Index: 8
#2Zhuchi Tu (JNU: Jinan University)H-Index: 7
Last.Xiao-Jiang Li (Emory University)H-Index: 62
view all 21 authors...
Summary Huntington's disease (HD) is characterized by preferential loss of the medium spiny neurons in the striatum. Using CRISPR/Cas9 and somatic nuclear transfer technology, we established a knockin (KI) pig model of HD that endogenously expresses full-length mutant huntingtin (HTT). By breeding this HD pig model, we have successfully obtained F1 and F2 generation KI pigs. Characterization of founder and F1 KI pigs shows consistent movement, behavioral abnormalities, and early death, which are...
44 CitationsSource
#1Su Yang (Emory University)H-Index: 9
#2Huiming Yang (CSU: Central South University)H-Index: 3
Last.Xiao-Jiang Li (Emory University)H-Index: 62
view all 10 authors...
The hypothalamus has a vital role in controlling food intake and energy homeostasis; its activity is modulated by neuropeptides and endocrine factors. Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a neurotrophic factor that is also localized in the endoplasmic reticulum (ER) in neurons. Here we show that MANF is highly enriched in distinct nuclei of the mouse hypothalamus, and that MANF expression in the hypothalamus is upregulated in response to fasting. Increasing or decreasing...
14 CitationsSource
#1Xiao-Jiang Li (Emory University)H-Index: 62
#2Zhuchi Tu (JNU: Jinan University)H-Index: 7
Last.Li Shihua (Emory University)H-Index: 83
view all 4 authors...
Off-target effects and mosaicism are major concerns for applying CRISPR-Cas9 to correct genetic mutations. A recent article in Nature by Ma et al. (2017) uses an elegant CRISPR-Cas9 approach that repairs a genetic mutation in human embryos with negligible mosaicism and no off-target effects, bringing this editing tool closer to clinical application.
2 CitationsSource
#1Zhuchi TuH-Index: 7
#2Weili YangH-Index: 9
Last.Xiao-Jiang LiH-Index: 62
view all 13 authors...
32 CitationsSource
#1Weili Yang (CAS: Chinese Academy of Sciences)H-Index: 9
#2Zhuchi Tu (CAS: Chinese Academy of Sciences)H-Index: 7
Last.Xiao-Jiang Li (CAS: Chinese Academy of Sciences)H-Index: 62
view all 4 authors...
CRISPR/Cas9 is now used widely to genetically modify the genomes of various species. The ability of CRISPR/Cas9 to delete DNA sequences and correct DNA mutations opens up a new avenue to treat genetic diseases that are caused by DNA mutations. In this review, we describe the advantages of using CRISPR/Cas9 to engineer genomic DNAs in animal embryos, as well as in specific regions or cell types in the brain. We also discuss how to apply CRISPR/Cas9 to establish animal models of neurodegenerative ...
20 CitationsSource
#1Zhuchi Tu (CAS: Chinese Academy of Sciences)H-Index: 7
#2Weili Yang (CAS: Chinese Academy of Sciences)H-Index: 9
Last.Xiao-Jiang Li (CAS: Chinese Academy of Sciences)H-Index: 62
view all 5 authors...
Animal models are extremely valuable to help us understand the pathogenesis of neurodegenerative disorders and to find treatments for them. Since large animals are more like humans than rodents, they make good models to identify the important pathological events that may be seen in humans but not in small animals; large animals are also very important for validating effective treatments or confirming therapeutic targets. Due to the lack of embryonic stem cell lines from large animals, it has bee...
53 CitationsSource
#1FENGMang (CAS: Chinese Academy of Sciences)H-Index: 24
#2Youpeng Zhong (ZJU: Zhejiang University)H-Index: 6
Last.Hangdong Wang (ZJU: Zhejiang University)H-Index: 19
view all 7 authors...
Quantum phase transitions play an important role in many-body systems and have been a research focus in conventional condensed-matter physics over the past few decades. Artificial atoms, such as superconducting qubits that can be individually manipulated, provide a new paradigm of realising and exploring quantum phase transitions by engineering an on-chip quantum simulator. Here we demonstrate experimentally the quantum critical behaviour in a highly controllable superconducting circuit, consist...
10 CitationsSource
#1Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
#2Yinghui Zheng (CAS: Chinese Academy of Sciences)H-Index: 3
Last.Xiao-Jiang Li (CAS: Chinese Academy of Sciences)H-Index: 62
view all 15 authors...
CRISPR/Cas9 has been used to genetically modify genomes in a variety of species, including non-human primates. Unfortunately, this new technology does cause mosaic mutations, and we do not yet know whether such mutations can functionally disrupt the targeted gene or cause the pathology seen in human disease. Addressing these issues is necessary if we are to generate large animal models of human diseases using CRISPR/Cas9. Here we used CRISPR/Cas9 to target the monkey dystrophin gene to create mu...
101 CitationsSource