Suvi Jain
Howard Hughes Medical Institute
Molecular biologyCTCFV(D)J recombinationBiologyImmunoglobulin heavy chain
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Publications 4
#1Zhaoqing Ba (HHMI: Howard Hughes Medical Institute)H-Index: 7
#2Jiangman Lou (HHMI: Howard Hughes Medical Institute)
Last. Frederick W. Alt (HHMI: Howard Hughes Medical Institute)H-Index: 156
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RAG endonuclease initiates V(D)J recombination in progenitor (pro)-B cells1. Upon binding a recombination center (RC)-based JH, RAG scans upstream chromatin via loop extrusion, potentially mediated by cohesin2-10, to locate Ds and assemble a DJH-based RC11. CTCF looping factor-bound elements (CBEs) within IGCR1 upstream of Ds impede RAG-scanning12-15; but their inactivation allows scanning to proximal VHs where additional CBEs activate rearrangement and impede scanning any further upstream15,16....
#1Suvi Jain (Boston Children's Hospital)H-Index: 3
#2Zhaoqing Ba (Boston Children's Hospital)H-Index: 7
Last. Frederick W. Alt (Boston Children's Hospital)H-Index: 156
view all 5 authors...
Summary RAG endonuclease initiates antibody heavy chain variable region exon assembly from V, D, and J segments within a chromosomal V(D)J recombination center (RC) by cleaving between paired gene segments and flanking recombination signal sequences (RSSs). The IGCR1 control region promotes DJ H intermediate formation by isolating Ds, J H s, and RCs from upstream V H s in a chromatin loop anchored by CTCF-binding elements (CBEs). How V H s access the DJ H RC for V H to DJ H rearrangement was unk...
20 CitationsSource
#1Ming Tian (Harvard University)H-Index: 19
#2Cheng Cheng (Vaccine Research Center)H-Index: 23
Last. Frederick W. Alt (Harvard University)H-Index: 156
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The design of immunogens that elicit broadly reactive neutralizing antibodies (bnAbs) has been a major obstacle to HIV-1 vaccine development. One approach to assess potential immunogens is to use mice expressing precursors of human bnAbs as vaccination models. The bnAbs of the VRC01-class derive from the IGHV1-2 immunoglobulin heavy chain and neutralize a wide spectrum of HIV-1 strains via targeting the CD4 binding site of the envelope glycoprotein gp120. We now describe a mouse vaccination mode...
87 CitationsSource
#1Chunguang Guo (Harvard University)H-Index: 13
#2Hye Suk Yoon (Harvard University)H-Index: 3
Last. Frederick W. Alt (Harvard University)H-Index: 156
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Immunoglobulin heavy chain (IgH) variable region exons are assembled from VH, D and JH gene segments in developing B lymphocytes. Within the 2.7-megabase mouse Igh locus, V(D)J recombination is regulated to ensure specific and diverse antibody repertoires. Here we report in mice a key Igh V(D)J recombination regulatory region, termed intergenic control region 1 (IGCR1), which lies between the VH and D clusters. Functionally, IGCR1 uses CTCF looping/insulator factor-binding elements and, correspo...
185 CitationsSource