Jean-Claude Gluckman
Centre national de la recherche scientifique
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Publications 123
#1Jean-Marc Sabatier (French Institute of Health and Medical Research)H-Index: 37
#2G. Fontan (French Institute of Health and Medical Research)H-Index: 3
Last. Jurphaas Van Rietschoten (French Institute of Health and Medical Research)H-Index: 7
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Chemical synthesis of large peptide fragments (from 18 to 66 amino acid residues long) of the gp110 envelope glycoprotein and of nef-protein from HIV-1 was achieved by the solid phase method. Stepwise assembling of the peptide chains was carried out automatically on 4-(oxymethyl)-phenylacetamidomethyl resin using the N-alpha-butyloxycarbonyl amino acids with benzyl-based side chain protecting groups. Two elongation protocols were used depending on the peptide chain length: a standard cycle, main...
15 CitationsSource
#1Emmanuel Fenouillet (CNRS: Centre national de la recherche scientifique)H-Index: 21
#2Blanes N (CNRS: Centre national de la recherche scientifique)H-Index: 4
Last. Jean-Claude Gluckman (CNRS: Centre national de la recherche scientifique)H-Index: 27
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SUMMARY During HIV-1 infection, the current tenet is that only anti-gag antibodies decline, while those directed at env remain stable. Among the latter antibodies, those directed to the V3 domain of gpl20 are assumed to play a role in the immune surveillance against HIV-1. We investigated the correlation between anti-V3 antibody levels and the clinical stage of infection and the ability to neutralize syncytium formation. Using a V3-specific antigen-limited ELISA, we analysed the antibody levels ...
11 CitationsSource
#2Bruno CanqueH-Index: 13
Last. Michelle RosenzwajgH-Index: 29
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Dendritic cells (DC) are essential antigen-presenting cells that initiate and regulate adaptive immune responses. There are distinct DC populations of diverse origins, which develop from hematopoietic progenitors already committed to the lymphoid or the myeloid lineages and, in the latter case, even from terminally differentiated macrophages. One may assume that DC of lymphoid origin are dedicated to the adaptive immune system, along which they have phylogenetically co-evolved, whereas myeloid D...
13 CitationsSource
#1M RosenzwajgH-Index: 1
#2N FeryH-Index: 1
Last. Jean-Claude GluckmanH-Index: 27
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Summary: Human herpes virus 8 (HHV8) may be sexually transmitted, but transmission via blood cells has not yet been excluded. We used a modified immunofluorescence assay to detect Ab to HHV8 latency-associated nuclear Ag in sera of 200 allogeneic BMT recipients and their related donors. In control subjects, Ab were found in 85% of patients with AIDS-related Kaposi sarcoma (n = 52), 34% of HIV-1 infected subjects without Kaposi sarcoma (n = 56) and 9.5% of blood donors (n = 42). Among BMT donors,...
43 CitationsSource
A live recombinant canarypox vector expressing HIV-1 gp120 MN tm/gag/protease LAI (ALVAC-HIV, vCP205) alone or boosted by a p24E-V3 MN synthetic peptide (CLTB-36) was tested in healthy volunteers at low risk for HIV infection for their safety and immunogenicity. Both antigens were well tolerated. ALVACHIV (vCP205) induced low levels of neutralizing antibodies against HIV-1 MN in 33% of the volunteers. None of them had detectable neutralizing antibodies against a nonsyncytium-inducing HIV-1 clade...
61 CitationsSource
4 CitationsSource
#1Michelle RosenzwajgH-Index: 29
Differentes methodes ont ete developpees pour obtenir des cellules dendritiques a partir de progeniteurs CD34+ ou de monocytes grâce a differentes combinaisons de cytokines qui comprennent principalement le GM-CSF, le TNFalpha, le SCF et l'interleukine 4.
#1Michelle RosenzwajgH-Index: 29
Les cellules dendritiques sont les plus efficaces des cellules presentatrices d'antigene. Elles seules sont capables d'induire la sensibilisation primaire de lymphocytes T "naifs". Elles peuvent egalement activer les lymphocytes T cytotoxiques specifiques d'antigenes viraux ou tumoraux et sont impliquees dans la regulation de la reponse immune humorale. Le paradigme est que les cellules dendritiques se differencient a partir de progeniteurs hematopoietiques dans la moelle osseuse, puis passent d...
2 Citations
#1Michelle RosenzwajgH-Index: 29
Dendritic cells are the most potent antigen-presenting cells. They, only, can prime naive T lymphocytes. They can also elicit generation of cytotoxic T lymphocytes against viral or tumour antigens and regulate humoral immune responses. The current paradigm is that dendritic cells differentiate from bone marrow progenitors and migrate via the blood to be distributed to tissues ; these immature dendritic cells have the capacity to capture and process antigens ; they then transit through the lymph ...
2 Citations