Chethan Ashokkumar
University of Pittsburgh
ImmunologyIntestine transplantationCD154TransplantationMedicine
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Publications 37
#1Chethan Ashokkumar (University of Pittsburgh)H-Index: 12
#2Michael Green (University of Pittsburgh)H-Index: 56
Last. Rakesh Sindhi (University of Pittsburgh)H-Index: 32
view all 27 authors...
Cell-mediated immunity to CMV, if known, could improve antiviral drug therapy in at-risk children and young adults with LT and IT. Host immunity has been measured with CMV-specific T cells, which express IFNgamma, but not those which express CD154, a possible substitute for IFNgamma. CMV-specific CD154+ T cells and their subsets were measured with flow cytometry after stimulating PBL from recipient blood samples with an overlapping peptide mix of CMV-pp65 antigen for up to 6 hours. CMV-specific ...
#1Neslihan Celik (University of Pittsburgh)H-Index: 2
#2Kaitlin Stanley (Boston Children's Hospital)H-Index: 1
Last. Rakesh Sindhi (University of Pittsburgh)H-Index: 32
view all 15 authors...
Abstract Transplantation of the intestine in children has presented significant challenges even as it has become a standard to treat nutritional failure due to short gut syndrome. These challenges have been addressed in part by significant improvements in short and long-term care. Noteworthy enhancements include reduced need for intestine transplantation, drug-sparing immunosuppressive regimens, immune monitoring, and improved surveillance and management of PTLD and non-adherence.
1 CitationsSource
#1Kyle Soltys (Boston Children's Hospital)H-Index: 22
#2Kentaro Setoyama (University of Pittsburgh)H-Index: 3
Last. Ira J. Fox (University of Pittsburgh)H-Index: 23
view all 35 authors...
Background & Aims Hepatocyte transplantation partially corrects genetic disorders and has been associated anecdotally with reversal of acute liver failure. Monitoring for graft function and rejection has been difficult, and has contributed to limited graft survival. Here we aimed to use preparative liver-directed radiation therapy, and continuous monitoring for possible rejection in an attempt to overcome these limitations. Methods Preparative hepatic irradiation was examined in non-human primat...
22 CitationsSource
#1Chethan AshokkumarH-Index: 12
#2Kyle SoltysH-Index: 22
Last. Rakesh SindhiH-Index: 32
view all 25 authors...
Allospecific CD154+T-cytotoxic memory cells (CD154+TcM) predict acute cellular rejection after liver transplantation (LTx) or intestine transplantation (ITx) in small cohorts of children and can enhance immunosuppression management, but await validation and clinical implementation.To establish safety and probable benefit, CD154+TcM were measured in cryopreserved samples from 214 children younger than 21 years (National Clinical Trial 1163578). Training set samples (n = 158) were tested with rese...
9 CitationsSource
#2Mylarappa NingappaH-Index: 10
Last. Rakesh SindhiH-Index: 32
view all 10 authors...
3 CitationsSource
#1Rakesh Sindhi (University of Pittsburgh)H-Index: 32
#2Chethan Ashokkumar (University of Pittsburgh)H-Index: 12
Last. Adriana Zeevi (Boston Children's Hospital)H-Index: 5
view all 9 authors...
SUMMARYThe PleximmuneTM test (Plexision Inc., Pittsburgh, PA, USA) is the first cell-based test approved by the US FDA, which predicts acute cellular rejection in children with liver- or intestine transplantation. The test addresses an unmet need to improve management of immunosuppression, which incurs greater risks of opportunistic infections and Epstein–Barr virus-induced malignancy during childhood. High-dose immunosuppression and recurrent rejection after intestine transplantation also resul...
5 CitationsSource
#1Mylarappa Ningappa (University of Pittsburgh)H-Index: 10
#2Chethan Ashokkumar (University of Pittsburgh)H-Index: 12
Last. Rakesh Sindhi (University of Pittsburgh)H-Index: 32
view all 12 authors...
T cell suppression prevents acute cellular rejection but causes life-threatening infections and malignancies. Previously, liver transplant (LTx) rejection in children was associated with the single-nucleotide polymorphism (SNP) rs9296068 upstream of the HLA-DOA gene. HLA-DOA inhibits B cell presentation of antigen, a potentially novel antirejection drug target. Using archived samples from 122 white pediatric LTx patients (including 77 described previously), we confirmed the association between r...
4 CitationsSource
#1Chethan AshokkumarH-Index: 12
#2Bishu GangulyH-Index: 1
Last. Rakesh SindhiH-Index: 32
view all 9 authors...
Belatacept blocks CD28-mediated T-cell costimulation and prevents renal transplant rejection. Understanding T-cell subset sensitivity to belatacept may identify cellular markers for immunosuppression failure to better guide treatment selection. Here, we evaluate the belatacept sensitivity of allo-antigen-specific CD154-expressing-T-cells, whose T-cytotoxic memory (TcM) subset predicts rejection with high sensitivity after non-renal transplantation. The belatacept concentration associated with ha...
2 CitationsSource
#1Mylarappa Ningappa (University of Pittsburgh)H-Index: 10
#2Ju-Hoon So (University of Pittsburgh)H-Index: 12
Last. Rakesh Sindhi (University of Pittsburgh)H-Index: 32
view all 20 authors...
Background & Aims Altered extrahepatic bile ducts, gut, and cardiovascular anomalies constitute the variable phenotype of biliary atresia (BA).
17 CitationsSource