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Rodrigo A. Valverde
Louisiana State University Agricultural Center
66Publications
20H-index
1,137Citations
Publications 66
Newest
#1Ricardo Iván Alcalá-Briseño (UF: University of Florida)H-Index: 2
#2Pongtharin Lotrakul (Chula: Chulalongkorn University)H-Index: 11
Last.Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
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A virus isolate from tabasco pepper (Capsicum frutescens) has been reported as a strain of the comovirus Andean potato mottle virus (APMoV). Using the replicative intermediate viral dsRNA, the pepper virus strain was sequenced by Illumina MiSeq. The viral genome was de novo assembled resulting in two RNAs with lengths of 6028 and 3646 nt. Nucleotide sequence analysis indicated that they corresponded to the RNA-1 and RNA-2 of a novel comovirus which we tentatively named pepper mild mosaic virus (...
#1Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
#2Mahmoud E. Khalifa (Damietta University)
Last.Sead Sabanadzovic (MSU: Mississippi State University)H-Index: 22
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The family Endornaviridae includes viruses with linear, single-stranded, positive-sense RNA genomes that range from 9.7 to 17.6 kb and have been reported infecting plants, fungi and oomycetes. The family consists of two genera, Alphaendornavirus and Betaendornavirus, into which viruses are classified based on their genome size, host and presence of unique domains. Alphaendornavirus includes species whose members infect plants, fungi and oomycetes, while the genus Betaendornavirus includes specie...
#1Rachel Herschlag (Louisiana State University Agricultural Center)
#2Cesar Escalante (Louisiana State University Agricultural Center)H-Index: 1
Last.Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
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Extraction and electrophoretic analysis of viral dsRNA from plants has been used successfully to detect infections by RNA viruses. We used this approach as an initial tool to test non-cultivated plant species for the presence of endornaviruses. Foliar samples were collected from symptomless plants in various locations within East Baton Rouge Parish, Louisiana, USA, and tested for viral dsRNA. After testing 208 plant species belonging to 74 families, five (Geranium carolinianum, Hydrocotyle umbel...
#1C. Escalante (Louisiana State University Agricultural Center)
#2Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
Abstract Plant endornaviruses are persistent viruses that infect plants without causing symptoms. Although endornaviruses have been reported in many economically important plant species, little is known about the effect they have on their hosts. Bell pepper endornavirus (BPEV) has been reported in cultivated peppers ( Capsicum spp.). In commercial cultivars of bell pepper ( C. annuum ) grown in the United States, this virus is found at prevalence rates of 100%. Two bell pepper near-isogenic line...
#1Cesar Escalante (Louisiana State University Agricultural Center)H-Index: 1
#2P. Bollich (Louisiana State University Agricultural Center)
Last.Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
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#1Ryo Okada (TUAT: Tokyo University of Agriculture and Technology)H-Index: 8
#2Ricardo Iván Alcalá-Briseño (UF: University of Florida)H-Index: 2
Last.Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
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Abstract The Endornaviridae family includes viruses with ssRNA genome that infect plants, fungi, and oomycetes. Plant endornaviruses do not cause visible symptoms and are transmitted only vertically. Many common bean ( Phaseolus vulgaris ) genotypes have been reported to be infected by Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2. Using next-generation sequencing, we obtained the RNA sequence of a third common bean endornavirus, which we named Phaseolus vulgaris endorn...
#1Ricardo Iván Alcalá-Briseño (UF: University of Florida)H-Index: 2
#2Ryo Okada (TUAT: Tokyo University of Agriculture and Technology)H-Index: 8
Last.Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
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Cluster bean (Cyamopsis tetragonoloba), also called guar, is a drought-tolerant annual legume. We conducted investigations to characterize a large dsRNA (~13-14 kbp) detected in a symptomless cluster bean genotype. The dsRNA was gel-purified and used for Illumina MiSeq sequencing. Reads were assembled, and BLASTx search results showed sequence similarity with viruses classified within the family Endornaviridae. The complete sequence of the putative endornavirus consisted of 12,895 nt and contain...
#1S. Khankhum (MSU: Mahasarakham University)H-Index: 1
#2Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
This study evaluated the physiological traits of eight lines of common bean (Phaseolus vulgaris) cv. Black Turtle Soup, four of which were double-infected with Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2, and four of which were endornavirus-free. Plants from all eight lines were morphologically similar and did not show statistically significant differences in plant height, wet weight, number of days to flowering and pod formation, pods per plant, pod thickness, seed s...
#1Cesar Escalante (Louisiana State University Agricultural Center)H-Index: 1
#2Ricardo Iván Alcalá-Briseño (UF: University of Florida)H-Index: 2
Last.Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
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#1S. Khankhum (Louisiana State University Agricultural Center)H-Index: 1
#2Cesar Escalante (Louisiana State University Agricultural Center)H-Index: 1
Last.Rodrigo A. Valverde (Louisiana State University Agricultural Center)H-Index: 20
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Extraction and electrophoretic analysis of large dsRNAs from plant and fungal tissues has been used successfully to detect RNA viruses infecting plants, fungi, and oomycetes. We modified a previously reported dsRNA extraction protocol and used it to detect a wide variety of plant and putative fungal viruses from infected plant tissues. The modified protocol was used successfully to extract large dsRNAs from 50 to 70 mg of desiccated plant tissues infected with acute and persistent RNA viruses an...
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