Mark R. Wills
University of Cambridge
Publications 80
#1Elizabeth Elder (University of Cambridge)H-Index: 1
#2Benjamin KrishnaH-Index: 9
Last.John Sinclair (University of Cambridge)H-Index: 41
view all 10 authors...
Human cytomegalovirus (HCMV) latency is an active process which remodels the latently infected cell to optimise latent carriage and reactivation. This is achieved, in part, through the expression of viral genes, including the G-protein coupled receptor US28. Here, we use an unbiased proteomic screen to assess changes in host proteins induced by US28, revealing that interferon-inducible genes are downregulated by US28. We validate that MHC Class II and two PYHIN proteins, MNDA and IFI16, are down...
#1Benjamin Krishna (University of Cambridge)H-Index: 9
#2Mark R. Wills (University of Cambridge)H-Index: 32
Last.John Sinclair (University of Cambridge)H-Index: 41
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#1Yusuf Aslam (University of Cambridge)H-Index: 1
#2James C Williamson (University of Cambridge)H-Index: 6
Last.Emma Poole (University of Cambridge)H-Index: 22
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Summary Human cytomegalovirus establishes a lifelong, latent infection in the human host and can cause significant morbidity and mortality, particularly, in immunocompromised individuals. One established site of HCMV latency and reactivation is in cells of the myeloid lineage. In undifferentiated myeloid cells, such as CD14+ monocytes, virus is maintained latently. We have recently reported an analysis of the total proteome of latently infected CD14+ monocytes, which identified an increase in he...
#1Sarah E. Jackson (University of Cambridge)H-Index: 17
#2George X. Sedikides (University of Cambridge)H-Index: 3
Last.Mark R. Wills (University of Cambridge)H-Index: 32
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Understanding how the T cell memory response directed towards human cytomegalovirus (HCMV) develops and changes over time while the virus persists is important. Whilst HCMV primary infection and periodic reactivation is well controlled by T cell responses in healthy people, when the immune system is compromised such as post-transplantation, during pregnancy, or underdeveloped such as in new-born infants and children, CMV disease can be a significant problem. In older people, HCMV infection is as...
#1Emma Poole (University of Cambridge)H-Index: 22
#2Christopher L.-H. Huang (University of Cambridge)H-Index: 43
Last.John Sinclair (University of Cambridge)H-Index: 41
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#1Suzan Fares (UCPH: University of Copenhagen)H-Index: 5
#2Katja Spiess (UCPH: University of Copenhagen)H-Index: 5
Last.Mette M. Rosenkilde (UCPH: University of Copenhagen)H-Index: 39
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ABSTRACT The Epstein-Barr virus (EBV) BILF1 gene encodes a constitutively active G protein-coupled receptor (GPCR) that downregulates major histocompatibility complex (MHC) class I and induces signaling-dependent tumorigenesis. Different BILF1 homologs display highly conserved extracellular loops (ECLs) including the conserved cysteine residues involved in disulfide bridges present in class A GPCRs (GPCR bridge between transmembrane helix 3 [TM-3] and ECL-2) and in chemokine receptors (CKR bridg...
#1Hoi Ping Mok (University of Cambridge)H-Index: 3
#2Nicholas J. Norton (University of Cambridge)H-Index: 2
Last.Andrew M. L. Lever (University of Cambridge)H-Index: 12
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The persistence of infected T cells harbouring intact HIV proviruses is the barrier to the eradication of HIV. This reservoir is stable over long periods of time despite antiretroviral therapy. There has been controversy on whether low level viral replication is occurring at sanctuary sites periodically reseeding infected cells into the latent reservoir to account its durability. To study viral evolution in a physiologically relevant population of latent viruses, we repeatedly performed virus ou...
#1Timokratis Karamitros (University of Oxford)H-Index: 6
#2Bonnie van Wilgenburg (University of Oxford)H-Index: 7
Last.Gkikas Magiorkinis (University of Oxford)H-Index: 22
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Background Human cytomegalovirus (HCMV) has a double-stranded DNA genome of approximately 235 Kbp that is structurally complex including extended GC-rich repeated regions. Genomic recombination events are frequent in HCMV cultures but have also been observed in vivo. Thus, the assembly of HCMV whole genomes from technologies producing shorter than 500 bp sequences is technically challenging. Here we improved the reconstruction of HCMV full genomes by means of a hybrid, de novo genome-assembly bi...