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Ahmad Usman Zafar
University of the Punjab
34Publications
11H-index
429Citations
Publications 34
Newest
#1Muhammad Umair Naseem (University of Debrecen)
#2Nadeem Ahmed (University of the Punjab)H-Index: 4
Last.Ahmad Usman Zafar (University of the Punjab)H-Index: 11
view all 5 authors...
Abstract Consensus interferon (cIFN) is a wholly synthetic therapeutic protein which is used to treat hepatitis C/B and certain types of malignancies. It has short serum half-life, therefore, to maintain its therapeutic level in the human body it requires thrice-weekly administration. Various strategies like PEGylation and micro-encapsulation have been developed during the last few years to enhance the pharmacokinetics of small therapeutic peptides. This study executed the human albumin-fusion t...
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#1Fakiha Bajwa (University of the Punjab)
#2Nadeem Ahmed (University of the Punjab)H-Index: 4
Last.Ahmad Usman Zafar (University of the Punjab)H-Index: 11
view all 7 authors...
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#1Rabbia Pervaiz (University of the Punjab)
#2Mohsin Khan (University of the Punjab)H-Index: 16
Last.Ahmad Usman Zafar (University of the Punjab)H-Index: 11
view all 5 authors...
Mosquitoes spread deadly infections around the world. Since decades Bacillus thuringiensis (Bt) δ-endotoxins have been used successfully as a biopesticide for controlling mosquito larvae. However, over a few years, mosquito larvae have evolved tolerance against Bt δ-endotoxins, rendering them ineffective for mosquito control. Such a problem entails the development of improved toxins with enhanced toxicity, affinity towards a wide range of mosquito receptors and ability to overcome or delay the r...
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#1Faidad Khan (University of the Punjab)H-Index: 3
#2Mohsin Khan (University of the Punjab)H-Index: 16
Last.Ahmad Usman Zafar (University of the Punjab)H-Index: 11
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Introduction Pakistan has one of the highest burdens of pneumococcal diseases in the world, but unfortunately studies in this demanding research area are limited in the region. Pneumococcal surface protein A (PspA) is the next generation pneumococcal vaccine candidate as the protein locates on the Streptococcus pneumoniae surface. Its gene, pspA, might be encoded by all pneumococci, and the protein has proven immunogenicity. The molecular characterization of PspA, pneumococcal serotype distribut...
1 CitationsSource
#1Nadeem Ahmed (University of the Punjab)H-Index: 4
#2Rabbia Abbas (University of the Punjab)
Last.Ahmad Usman Zafar (University of the Punjab)H-Index: 11
view all 6 authors...
Interleukin-6 a pleiotropic cytokine involved in wide range biological activities. So the large scale Production of biologically active recombinant human interleukin-6 is important for its structural and functional studies. Here we report an optimized method for shake flask fermentation and simplified high yield purification procedure for the recombinant interleukin -6. This high yield expression method not only involves the optimization of the fermentation condition, but also the single step pu...
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#1Mohsin Khan (University of the Punjab)H-Index: 16
#2Nadeem Ahmed (University of the Punjab)H-Index: 4
Last.Tayyab Husnain (University of the Punjab)H-Index: 25
view all 9 authors...
Previously, we have reported cloning of human epidermal growth factor gene from Huh-7 cells and its extracellular expression in Pichia pastoris. The presented work is a detailed report regarding molecular characterization of Huh-7 cells-derived hEGF expressed in Pichia pastoris with special reference to its glycosylation profiling and bioactivity studies. Densitometric scanning of SDS-PAGE separated extracellular proteins from hEGF recombinant Pichia pastoris strain indicated that about 84% of t...
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#1Hamid Bashir (University of the Punjab)H-Index: 3
#2Nadeem Ahmed (University of the Punjab)H-Index: 4
Last.Tayyab Husnain (University of the Punjab)H-Index: 25
view all 9 authors...
Recent advancement in technologies like fermentation procedures resulted in the increased yields of recombinant proteins of biopharmaceutical and medicinal importance. Consequently, there is an important task to develop simple and easily scalable methods that facilitate the production of high quality recombinant protein. Most of the recent reports described the expression of Recombinant human IL-1receptor antagonist in Escherichia coli using isopropyl-β-D-thiogalacto pyranoside (IPTG), a non-met...
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#1Hamid Bashir (University of the Punjab)H-Index: 3
#2Nadeem Ahmed (University of the Punjab)H-Index: 4
Last.Tayyab Husnain (University of the Punjab)H-Index: 25
view all 8 authors...
Recombinant consensus interferon (CIFN) is a therapeutic protein with molecular weight of 19.5 kDa having broad spectrum antiviral activity. Recombinant human CIFN (rhCIFN) has previously been expressed in Escherichia coli using isopropyl-β-d-thiogalactopyranoside (IPTG), a non-metabolizable and expensive compound, as inducer. For economical and commercial-scale recombinant protein production, it is greatly needed to increase the product yield in a limited time frame to reduce the processing cos...
7 CitationsSource
#1Nadeem Ahmed (University of the Punjab)H-Index: 4
#2Hamid Bashir (University of the Punjab)H-Index: 3
Last.Tayyab Husnain (University of the Punjab)H-Index: 25
view all 9 authors...
Recombinant human consensus interferon (rh-cIFN) is an artificially engineered interferon (IFN) developed by recombining and reordering the protein sequences that exist in standard IFN. This recombination resulted into a drug that has the potential to work better than natural, standard IFN. In this study, we described optimized conditions for high-level expression and recovery of biologically active consensus IFN from inclusion bodies (IBs). A synthetic gene coding 166 amino acids of consensus I...
6 CitationsSource
#1Nadeem Ahmed (University of the Punjab)H-Index: 4
#2Ahmad Usman Zafar (University of the Punjab)H-Index: 11
Last.Tayyab Husnain (University of the Punjab)H-Index: 25
view all 10 authors...
Biological activity of human interleukin-6 (IL-6) is associated with a vast number of diseases such as rheumatoid arthritis, sepsis, and severe inflammatory diseases. In this study, human IL-6 cDNA was isolated from a cDNA library that was constructed with mRNA derived from human placental tissues. Subsequently, the complete human IL-6 cDNA was cloned and expressed in BL21DE3 cells. The recombinant human IL-6 (rhIL-6) protein was expressed in a form of an insoluble inclusion body. Inclusion bodi...
3 CitationsSource
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