Match!
Patrik Lundquist
Uppsala University
TransporterIn vivoBiochemistryBiologyPharmacology
15Publications
8H-index
240Citations
What is this?
Publications 16
Newest
#1Christine Wegler (AstraZeneca)H-Index: 5
#2Magnus Ölander (Uppsala University)H-Index: 2
Last. Per Artursson (Science for Life Laboratory)H-Index: 77
view all 4 authors...
Genes and proteins show variable expression patterns throughout the human body. However, it is not clear whether relative differences in mRNA concentrations are retained on the protein level. Furth ...
Source
#1Per Artursson (Uppsala University)H-Index: 77
#2Patrik Lundquist (Uppsala University)H-Index: 8
Negatively charged nanoparticles of about 50 nm in size permit the oral delivery of insulin and other peptide drugs by temporally enhancing the permeability of the intestinal wall.
Source
#1Christine WeglerH-Index: 5
#2Magnus ÖlanderH-Index: 2
Last. Per ArturssonH-Index: 77
view all 9 authors...
#1Neha Shrestha (UCL: Université catholique de Louvain)H-Index: 12
#2Oriane Bouttefeux (UCL: Université catholique de Louvain)H-Index: 2
Last. Ana Beloqui (UCL: Université catholique de Louvain)H-Index: 10
view all 13 authors...
Nanoparticulate based drug delivery systems have been extensively studied to efficiently encapsulate and deliver peptides orally. However, most of the existing data mainly focus on the nanoparticles as a drug carrier, but the ability of nanoparticles having a biological effect has not been exploited. Herein, we hypothesize that nanostructured lipid carriers (NLCs) could activate the endogenous glucagon-like peptide-1 (GLP-1) secretion and also act as oral delivery systems for GLP-1 analogs (exen...
7 CitationsSource
#1Maria Karlgren (Uppsala University)H-Index: 7
#2Ivailo Simoff (Uppsala University)H-Index: 3
Last. Per Artursson (Uppsala University)H-Index: 77
view all 11 authors...
Abstract Madin-Darby canine kidney (MDCK) II cells stably transfected with transport proteins are commonly used models for drug transport studies. However, endogenous expression of especially canine MDR1 (cMDR1) confounds the interpretation of such studies. Here we have established an MDCK cell line stably overexpressing the human MDR1 transporter (hMDR1; P-glycoprotein), and used CRISPR-Cas9 gene editing to knockout the endogenous cMDR1. Genomic screening revealed the generation of a clonal cel...
8 CitationsSource
#1Patrik Lundquist (Uppsala University)H-Index: 8
#2Per Artursson (Uppsala University)H-Index: 77
Abstract In this contribution, we review the molecular and physiological barriers to oral delivery of peptides and nanoparticles. We discuss the opportunities and predictivity of various in vitro systems with special emphasis on human intestine in Ussing chambers. First, the molecular constraints to peptide absorption are discussed. Then the physiological barriers to peptide delivery are examined. These include the gastric and intestinal environment, the mucus barrier, tight junctions between ep...
94 CitationsSource
#1Pär Matsson (Uppsala University)H-Index: 19
#2Patrik Lundquist (Uppsala University)H-Index: 8
Last. Per Artursson (Uppsala University)H-Index: 77
view all 3 authors...
We recently published an analysis in Trends in Pharmacological Sciences, assessing the impact of transporters on cellular drug permeability based on quantitative data for drug transport kinetics and transporter expression levels [1,2]. Our aim was to explore what properties transporters would need to have if – as hypothesized in a series of publications over the past few years – drug diffusion across the lipid parts of cell membranes is negligible, and transporter-mediated processes explain all ...
1 CitationsSource
#1Magnus Ölander (Uppsala University)H-Index: 2
#2Jacek R. Wiśniewski (MPG: Max Planck Society)H-Index: 25
Last. Per Artursson (Uppsala University)H-Index: 77
view all 5 authors...
Caco-2 cells are widely used in studies of intestinal cell physiology and drug transport. Here, the global proteome of filter-grown Caco-2 cells was quantified using the total protein approach and compared with the human colon and jejunum proteomes. In total, 8096 proteins were identified. In-depth analysis of proteins defining enterocyte differentiation—including brush-border hydrolases, integrins, and adherens and tight junctions—gave near-complete coverage of the expected proteins. Three hund...
22 CitationsSource
#1Jacek R. WisniewskiH-Index: 24
#2Pär MatssonH-Index: 19
Last. Per ArturssonH-Index: 77
view all 4 authors...
Caco-2 cells are widely used in studies of intestinal cell physiology and drug transport. Here, the global proteome of filter-grown Caco-2 cells was quantified using the total protein approach and compared with the human colon and jejunum proteomes. In total, 8096 proteins were identified. In-depth analysis of proteins defining enterocyte differentiationdincluding brush-border hydrolases, integrins, and adherens and tight junctionsdgave near-complete coverage of the expected proteins. Three hund...
#1Pär Matsson (Uppsala University)H-Index: 19
#2Luca A. Fenu (Uppsala University)H-Index: 4
Last. Per Artursson (Uppsala University)H-Index: 77
view all 6 authors...
We recently published an analysis of the limits of carrier-mediated drug transport and whether transporters alone can explain experimental observations that are typically rationalized by drug diffusion through the lipid portions of cell membranes [1]. We found that carrier-mediated transport rates can be high enough to explain observed rates for high-permeability drugs across monolayers of Caco-2 cells, but only if the drugs are substrates of transporters with extreme capacity or if transport is...
3 CitationsSource
12