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Jan Pieter Abrahams
Leiden University
Molecular biologyChemistryBiochemistryCrystallographyBiology
200Publications
38H-index
8,032Citations
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Publications 191
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#1Sevasti Filippidou (OU: Open University)
Last. Shannon L. Johnson (LANL: Los Alamos National Laboratory)H-Index: 22
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Sporulation is a complex morphophysiological process resulting in a cellular structure that is more resistant than the vegetative form. In Firmicutes, this structure is produced within the mother cell, and is called an endospore. Endospore formation is thought to have evolved in the common ancestor of Firmicutes. However, sporulation has apparently been lost in some extant lineages that are defined as asporogenic. We isolated strain 11kri321, a representative of the genus Kurthia, from an oligot...
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#1Zhenzhen Zhang (CPU: China Pharmaceutical University)
#2Kai Wen (CPU: China Pharmaceutical University)
Last. Xiang Zhou (CPU: China Pharmaceutical University)
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It is a hot topic for improving efficiency and decreasing the toxicity of gene transfection reagents. The extracellular nanovesicles (EVs) that are released by cells play an important role in intercellular communication, and naturally designed for genetic exchange between cells. Here we show that the EVs have a large beneficial effect in polyethyleneimine (PEI)-mediated transfection of a GFP-encoding plasmid into HEK293T cells. An improvement as high as ~ 500%, as well as a decrease in toxicity,...
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#1Joachim M. Matz (Francis Crick Institute)H-Index: 5
#1Joachim Michael Matz (Francis Crick Institute)
Last. Kai Matuschewski (Humboldt University of Berlin)H-Index: 42
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During blood stage development, malaria parasites are challenged with the detoxification of enormous amounts of haem released during the proteolytic catabolism of erythrocytic haemoglobin. They tackle this problem by sequestering haem into bioinert crystals known as haemozoin. The mechanisms underlying this biomineralization process remain enigmatic. Here, we demonstrate that both rodent and human malaria parasite species secrete and internalize a lipocalin-like protein, PV5, to control haem cry...
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#1Andrea Merg (Emory University)H-Index: 1
#1Andrea D. Merg (Emory University)H-Index: 5
Last. Vincent P. Conticello (Emory University)H-Index: 36
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Engineering free-standing 2D nanomaterials with compositional, spatial, and functional control across size regimes from the nano- to mesoscale represents a significant challenge. Herein, we demonstrate a straightforward strategy for the thermodynamically controlled fabrication of multicomponent “sectored” nanosheets in which each sector can be chemically and spatially addressed independently and orthogonally. Collagen triple helices, comprising collagen-mimetic peptides (CMPs), are employed as m...
1 CitationsSource
#1Xiansha Xiao (LEI: Leiden University)
#2Joost Willemse (LEI: Leiden University)H-Index: 16
Last. Gilles P. van Wezel (LEI: Leiden University)H-Index: 43
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In most bacteria, cell division begins with the polymerization of the GTPase FtsZ at the mid-cell, which recruits the division machinery to initiate cell constriction. In the filamentous bacterium Streptomyces, cell division is positively controlled by SsgB, which recruits FtsZ to the future septum sites and promotes Z-ring formation. Here we show via site-saturated mutagenesis that various amino acid substitutions in the highly conserved SsgB protein result in the production of ectopically plac...
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#1Thorsten B. BlumH-Index: 1
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#1Thorsten B. Blum (University of Basel)H-Index: 1
#2Sevasti FilippidouH-Index: 6
Last. Jan Pieter Abrahams (University of Basel)H-Index: 38
view all 5 authors...
Bacteria swim and swarm by rotating the micrometers long, helical filaments of their flagella. They change direction by reversing their flagellar rotation, which switches the handedness of the filament’s supercoil. So far, all studied functional filaments are composed of a mixture of L- and R-state flagellin monomers. Here we show in a study of the wild type Firmicute Kurthia sp., that curved, functional filaments can adopt a conformation in vivo that is closely related to a uniform, all-L-state...
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#1Andrea Merg (Emory University)H-Index: 1
#1Andrea D. Merg (Emory University)H-Index: 5
Last. Vincent P. Conticello (Emory University)H-Index: 36
view all 10 authors...
: The successful integration of 2D nanomaterials into functional devices hinges on developing fabrication methods that afford hierarchical control across length scales of the entire assembly. We demonstrate structural control over a class of crystalline 2D nanosheets assembled from collagen triple helices. By lengthening the triple helix unit through sequential additions of Pro-Hyp-Gly triads, we achieved sub-angstrom tuning over the 2D lattice. These subtle changes influence the overall nanoshe...
3 CitationsSource
Multi-slice simulations of electron diffraction by three-dimensional protein crystals have indicated that structure solution would be severely impeded by dynamical diffraction, especially when crystals are more than a few unit cells thick. In practice, however, dynamical diffraction turned out to be less of a problem than anticipated on the basis of these simulations. Here it is shown that two scattering phenomena, which are usually omitted from multi-slice simulations, reduce the dynamical effe...
1 CitationsSource
#1Mauro Gemmi (IIT: Istituto Italiano di Tecnologia)H-Index: 25
#2Enrico Mugnaioli (IIT: Istituto Italiano di Tecnologia)H-Index: 24
Last. Jan Pieter Abrahams (University of Basel)H-Index: 38
view all 8 authors...
Crystallography of nanocrystalline materials has witnessed a true revolution in the past 10 years, thanks to the introduction of protocols for 3D acquisition and analysis of electron diffraction data. This method provides single-crystal data of structure solution and refinement quality, allowing the atomic structure determination of those materials that remained hitherto unknown because of their limited crystallinity. Several experimental protocols exist, which share the common idea of sampling ...
14 CitationsSource
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