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Chi Bun Ching
National University of Singapore
186Publications
35H-index
5,138Citations
Publications 180
Newest
#1Jifeng Yuan (Agency for Science, Technology and Research)H-Index: 7
#2Xue Chen (NUS: National University of Singapore)H-Index: 1
Last.Chi Bun Ching (NUS: National University of Singapore)H-Index: 35
view all 4 authors...
Higher chain alcohols have gained much attention as next generation transport fuels because of their higher energy density and low moisture absorption capacity compared to ethanol. In the present study, we attempted to engineer Saccharomyces cerevisiae for the synthesis of isoamyl alcohol via de novo leucine biosynthetic pathway coupled with Ehrlich degradation pathway. To achieve high-level production of isoamyl alcohol, two strategies are used in the current study: (1) reconstruction of a chro...
6 CitationsSource
#1Jifeng Yuan (Agency for Science, Technology and Research)H-Index: 7
#2Pranjul Mishra (NUS: National University of Singapore)H-Index: 4
Last.Chi Bun Ching (NUS: National University of Singapore)H-Index: 35
view all 3 authors...
Isoamyl alcohol can be used not only as a biofuel, but also as a precursor for various chemicals. Saccharomyces cerevisiae inherently produces a small amount of isoamyl alcohol via the leucine degradation pathway, but the yield is very low. In the current study, several strategies were devised to overproduce isoamyl alcohol in budding yeast. The engineered yeast cells with the cytosolic isoamyl alcohol biosynthetic pathway produced significantly higher amounts of isobutanol over isoamyl alcohol,...
3 CitationsSource
#1Wen XuH-Index: 2
#2Jifeng YuanH-Index: 7
Last.Jiankang LiuH-Index: 59
view all 5 authors...
Microbial synthesis of ubiquinone by fermentation processes has been emerging in recent years. However, as ubiquinone is a primary metabolite that is tightly regulated by the host central metabolism, tweaking the individual pathway components could only result in a marginal improvement on the ubiquinone production. Given that ubiquinone is stored in the lipid bilayer, we hypothesized that introducing additional metabolic sink for storing ubiquinone might improve the CoQ10 production. As human li...
3 CitationsSource
#1Jifeng Yuan (Agency for Science, Technology and Research)H-Index: 7
#2Pranjul Mishra (NUS: National University of Singapore)H-Index: 4
Last.Chi Bun Ching (NUS: National University of Singapore)H-Index: 35
view all 3 authors...
Abstract Medium branched-chain esters can be used not only as a biofuel but are also useful chemicals with various industrial applications. The development of economically feasible and environment friendly bio-based fuels requires efficient cell factories capable of producing desired products in high yield. Herein, we sought to use a number of strategies to engineer Saccharomyces cerevisiae for high-level production of branched-chain esters. Mitochondrion-based expression of ATF1 gene in a base ...
5 CitationsSource
#1Jifeng Yuan (NUS: National University of Singapore)H-Index: 7
#2Chi Bun Ching (NUS: National University of Singapore)H-Index: 35
Acetyl-CoA is a central molecule in the metabolism of the cell, which is also a precursor molecule to a variety of value-added products such as terpenoids and fatty acid derived molecules. Considering subcellular compartmentalization of metabolic pathways allows higher concentrations of enzymes, substrates and intermediates, and bypasses competing pathways, mitochondrion-compartmentalized acetyl-CoA utilization pathways might offer better pathway activities with improved product yields. As a pro...
15 CitationsSource
#1Pranjul Mishra (NUS: National University of Singapore)H-Index: 4
#2Gyuyeon ParkH-Index: 2
Last.Dong-Yup Lee (Agency for Science, Technology and Research)H-Index: 31
view all 9 authors...
Recently, the bio-production of α,ω-dicarboxylic acids (DCAs) has gained significant attention, which potentially leads to the replacement of the conventional petroleum-based products. In this regard, the lipid accumulating yeast Candida tropicalis, has been recognized as a promising microbial host for DCA biosynthesis: it possess the unique ω-oxidation pathway where the terminal carbon of α-fatty acids is oxidized to form DCAs with varying chain lengths. However, despite such industrial importa...
23 CitationsSource
#1Jifeng Yuan (NUS: National University of Singapore)H-Index: 7
#2Chi Bun Ching (NUS: National University of Singapore)H-Index: 35
Background To achieve high-level production of non-native isoprenoid products, it requires the metabolic flux to be diverted from the production of sterols to the heterologous metabolic reactions. However, there are limited tools for restricting metabolic flux towards ergosterol synthesis. In the present study, we explored dynamic control of ERG9 expression using different ergosterol-responsive promoters to improve the production of non-native isoprenoids.
43 CitationsSource
#1Guan Liu (SUES: Shanghai University of Engineering Sciences)H-Index: 1
#2Lijuan Zhang (SUES: Shanghai University of Engineering Sciences)H-Index: 8
Last.Jie Lu (SUES: Shanghai University of Engineering Sciences)H-Index: 17
view all 6 authors...
Abstract Zwitterionic (ZI) membranes are well-known for their high resistance to protein fouling during ultrafiltration (UF). In this work, zwitterionic chitosan (ZICS) was firstly obtained by a Schiff-base formation and a sulfonation reaction. Then a series of zwitterionic organic–inorganic hybrid ultrafiltration membranes were prepared through linking ZICS with poly vinyl alcohol (PVA) using tetraethyl orthosilicate as a bridge, where the density of zwitterionic groups in the membranes was adj...
24 CitationsSource
Saccharomyces cerevisiae as a eukaryotic organism is particularly suitable as microbial cell factory because it has interesting features such as membrane environments for supporting membrane-associated enzymes and its capability for post-translational modifications of enzymes from plants. However, S. cerevisiae does not readily express polycistronic transcriptional units, which represents a significant challenge for constructing large biochemical pathways in budding yeast. In the present study, ...
30 CitationsSource
#1Jifeng Yuan (NUS: National University of Singapore)H-Index: 7
#2Chi Bun Ching (NUS: National University of Singapore)H-Index: 35
Combinatorial genome integration of mevalonate pathway genes was performed with the aim of optimizing the metabolic flux for improved production of terpenoids in budding yeast. In the present study, we developed a novel δ-integration platform to achieve multiple genome integrations through modulating the concentration of antibiotics. By exploiting carotenoid biosynthesis as screening module, we successfully created a library of yeast colonies appeared with various intensities of orange color. As...
27 CitationsSource
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