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Yijun Cai
Chinese Academy of Sciences
Genome editingMolecular biologyPrimateGeneticsBiology
14Publications
8H-index
402Citations
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Publications 14
Newest
#1Peiyuan Qiu (CAS: Chinese Academy of Sciences)H-Index: 1
#2Jian Jiang (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Hung-Chun Chang (CAS: Chinese Academy of Sciences)H-Index: 14
view all 15 authors...
Circadian disruption is a risk factor for metabolic, psychiatric and age-related disorders, and non-human primate models could help to develop therapeutic treatments. Here, we report the generation of BMAL1 knockout cynomolgus monkeys for circadian-related disorders by CRISPR/Cas9 editing of monkey embryos. These monkeys showed higher nocturnal locomotion and reduced sleep, which was further exacerbated by a constant light regimen. Physiological circadian disruption was reflected by the markedly...
6 CitationsSource
#1Zhen Liu (CAS: Chinese Academy of Sciences)H-Index: 12
#2Yijun Cai (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Qiang Sun (CAS: Chinese Academy of Sciences)H-Index: 6
view all 16 authors...
Cloning of macaque monkeys by somatic cell nucleus transfer (SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases. Here, we report the feasibility of this approach by SCNT of fibroblasts from a macaque monkey (Macaca fascicularis), in which a core circadian transcription factor BMAL1 was knocked out by clustered regularly interspaced short palindromic repeat/Cas9 gene editing (see accompanying pa...
9 CitationsSource
#1Hui Long (SJTU: Shanghai Jiao Tong University)H-Index: 6
#2Yan Wang (CAS: Chinese Academy of Sciences)H-Index: 9
Last. Qiang Sun (CAS: Chinese Academy of Sciences)H-Index: 6
view all 11 authors...
Abstract AMH is regarded as a promising predictor for ovarian reserve in humans and non-human primate, and widely used in human medicine to predict ovarian response to gonadotropin, menopause and premature ovarian failure. However, large data set on the range of AMH levels in nonhuman primates is still scarce, which limited its applications largely. In this study, age-related AMH nomograms of rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) were produced and compared. 219 rhesus and ...
Source
#1Jinlin Su (CAS: Chinese Academy of Sciences)H-Index: 1
#2Pei Huang (SJTU: Shanghai Jiao Tong University)H-Index: 2
Last. Jin Xu (CAS: Chinese Academy of Sciences)H-Index: 14
view all 15 authors...
Huntingtin interaction protein 2 (HIP2) is an E2 ubiquitin-conjugating enzyme associated with neurodegenerative diseases, and HIP2 mRNA has been implicated as a potential blood biomarker for Parkinson’s disease (PD). However, it is unclear whether the alteration of HIP2 expression may contribute to the development of PD, and whether the change of HIP2 in blood could reflect its expression in the brain or motor functions in PD patients. In this study, we established a mouse line with HIP2 haploin...
2 CitationsSource
#1Hui LongH-Index: 6
#2Yan-Hong NieH-Index: 8
Last. Qiang SunH-Index: 6
view all 11 authors...
Source
#1Zhen Liu (CAS: Chinese Academy of Sciences)H-Index: 12
#2Yijun Cai (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Qiang Sun (CAS: Chinese Academy of Sciences)H-Index: 6
view all 11 authors...
Summary Generation of genetically uniform non-human primates may help to establish animal models for primate biology and biomedical research. In this study, we have successfully cloned cynomolgus monkeys ( Macaca fascicularis ) by somatic cell nuclear transfer (SCNT). We found that injection of H3K9me3 demethylase Kdm4d mRNA and treatment with histone deacetylase inhibitor trichostatin A at one-cell stage following SCNT greatly improved blastocyst development and pregnancy rate of transplanted S...
63 CitationsSource
#1Yonglu Tian (PKU: Peking University)H-Index: 2
#2Chaojuan Yang (McGovern Institute for Brain Research)H-Index: 1
Last. Chen Zhang (McGovern Institute for Brain Research)H-Index: 2
view all 14 authors...
Fragile X syndrome (FXS) is a neurodevelopmental disorder caused by mutations in the FMR1 gene that inactivate expression of the gene product, the fragile X mental retardation 1 protein (FMRP). In this study, we used clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology to generate Fmr1 knockout (KO) rats by disruption of the fourth exon of the Fmr1 gene. Western blotting analysis confirmed that the FMRP was absent from the brains of the...
17 CitationsSource
#1Erwei Zuo (CAS: Chinese Academy of Sciences)H-Index: 6
#2Yijun Cai (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 29 authors...
One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
47 CitationsSource
#1Zhen Liu (CAS: Chinese Academy of Sciences)H-Index: 12
#2Yijun Cai (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Qiang Sun (CAS: Chinese Academy of Sciences)H-Index: 6
view all 3 authors...
2 CitationsSource
#1Zhen LiuH-Index: 12
#2Xiao LiH-Index: 6
Last. Zilong QiuH-Index: 25
view all 26 authors...
Lentivirus-based transgenic Macaca fascicularis monkeys are generated expressing the human MECP2 transgene in the brain, and they display behavioural alterations including changes in social interaction and increased anxiety; germline transmission of the transgene to the F1 offspring is shown, and these monkeys also had an altered social interaction phenotype.
110 CitationsSource
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