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Chetan Sood
Emory University
Fusion mechanismVirusViral membraneBiochemistryBiology
9Publications
4H-index
59Citations
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Publications 11
Newest
#1Yen-Cheng ChenH-Index: 1
#2Chetan SoodH-Index: 4
Last. Gregory B. MelikyanH-Index: 23
view all 7 authors...
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#1Yen-Cheng Chen (Emory University)H-Index: 3
#2Chetan Sood (Emory University)H-Index: 4
Last. Robert M. Dickson (Georgia Institute of Technology)H-Index: 38
view all 5 authors...
Live cell fluorescence imaging is the method of choice for studying dynamic processes, such as nuclear transport, vesicular trafficking, and virus entry and egress. However, endogenous cellular autofluorescence masks a useful fluorescence signal, limiting the ability to reliably visualize low-abundance fluorescent proteins. Here, we employed synchronously amplified fluorescence image recovery (SAFIRe), which optically alters ground versus photophysical dark state populations within fluorescent p...
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#1Yen-Cheng ChenH-Index: 1
#2Chetan SoodH-Index: 4
Last. Gregory B. MelikyanH-Index: 23
view all 4 authors...
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#1Chetan SoodH-Index: 4
Last. Gregory B. MelikyanH-Index: 23
view all 4 authors...
Source
#1Chetan Sood (Emory University)H-Index: 4
#2Ashwanth C. Francis (Emory University)H-Index: 5
Last. Gregory B. Melikyan (Emory University)H-Index: 23
view all 4 authors...
Abstract Enveloped viruses transfer their genomes into host cells by fusing their membrane to that of the cell. To visualize single-virus fusion in living cells, researchers take advantage of HIV-1's proteolytic maturation, which can generate free fluorescent proteins within the viral particle. Co-labeling viruses with a content marker and a fluorescently tagged Vpr (a viral core protein) enables detection of single-virus fusions, but a major limitation of this approach is that not all viral par...
7 CitationsSource
#1Chetan Sood (Emory University)H-Index: 4
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory B. Melikyan (Emory University)H-Index: 23
view all 5 authors...
Abstract The host proteins, SERINC3 and SERINC5, have been recently shown to incorporate into HIV-1 particles and compromise their ability to fuse with target cells, an effect that is antagonized by the viral Nef protein. Envelope (Env) glycoproteins from different HIV-1 isolates exhibit a broad range of sensitivity to SERINC-mediated restriction, and the mechanism by which SERINCs interfere with HIV-1 fusion remains unclear. Here, we show that incorporation of SERINC5 into virions in the absenc...
28 CitationsSource
#1Chetan Sood (Emory University)H-Index: 4
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory Melikian (Emory University)
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The multispan membrane proteins, SERINC3 and SERINC5, have been recently shown to incorporate into HIV-1 particles and compromise their ability to fuse with target cells – an effect that is antagonized by the viral accessary protein Nef. Env glycoproteins from different HIV-1 strains exhibit variable levels of sensitivity to SERINC-mediated restriction. The mechanism by which SERINCs interfere with HIV-1 fusion remains unclear. Here, we show by real-time single particle imaging that incorporatio...
Source
#1Chetan Sood (Emory University)H-Index: 4
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory B. Melikyan (Emory University)H-Index: 23
view all 4 authors...
HIV-1 fusion leading to productive entry has long been thought to occur at the plasma membrane. However, our previous single virus imaging data imply that, after Env engagement of CD4 and coreceptors at the cell surface, the virus enters into and fuses with intracellular compartments. We were unable to reliably detect viral fusion at the plasma membrane. Here, we implement a novel virus labeling strategy that biases towards detection of virus fusion that occurs in a pH-neutral environment—at the...
6 CitationsSource
#1Chetan Sood (Emory University)H-Index: 4
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory B. Melikyan (Emory University)H-Index: 23
view all 4 authors...
The HIV-1 infectious cycle begins with viral glycoprotein mediated fusion with the host cell membrane and subsequent entry of the viral capsid to the host cytosol. It has been proposed that HIV-1 fusion and productive entry occur at the plasma membrane (PM) because functionality of the HIV-1 fusion glycoprotein (Env) is pH-independent. Further supporting this hypothesis are the observations that HIV-1 mediates fusion between adjacent cells and that cell-cell fusion occurs between Env- and recept...
Source
#1Tanay M. Desai (Emory University)H-Index: 8
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory B. Melikyan (Emory University)H-Index: 23
view all 7 authors...
Background HIV-1 Vpr is recruited into virions during assembly and appears to remain associated with the viral core after the reverse transcription and uncoating steps of entry. This feature has prompted the use of fluorescently labeled Vpr to visualize viral particles and to follow trafficking of post-fusion HIV-1 cores in the cytoplasm.
18 CitationsSource
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