Match!
Aneta Manda-Handzlik
Medical University of Warsaw
Neutrophil extracellular trapsPhagocytosisImmunologyMedicineBiology
17Publications
7H-index
113Citations
What is this?
Publications 17
Newest
#1Monika Bil (Warsaw University of Technology)H-Index: 6
#2Ewa Kijeńska-Gawrońska (Warsaw University of Technology)H-Index: 5
Last. Piotr Mrowka (Medical University of Warsaw)H-Index: 8
view all 5 authors...
Abstract Integration of multiple features including shape memory, biodegradation, and sustained drug delivery in a single material offers the opportunity to significantly improve the abilities of implantable devices for cardiovascular system regeneration. Two types of shape memory polyurethanes (SMPUs): PU-PLGA and PU-PLLA/PEG differing in soft segments composition that comprising blends of various biodegradable polyols, i.e. D, l -lactide-co-glycolide diol (o-PLGA), poly(e-caprolactone) diols (...
1 CitationsSource
#1Aneta Manda-Handzlik (Medical University of Warsaw)H-Index: 7
Last. Urszula Demkow (Medical University of Warsaw)H-Index: 22
view all 5 authors...
Over a decade ago, the formation of neutrophil extracellular traps (NETs) was described as a novel mechanism employed by neutrophils to tackle infections. Currently applied methods for NETs release quantification are often limited by the use of unspecific dyes and technical difficulties. Therefore, we aimed to develop a fully automatic image processing method for the detection and quantification of NETs based on live imaging with the use of DNA-staining dyes. For this purpose, we adopted a recen...
Source
#1Aneta Manda-Handzlik (Medical University of Warsaw)H-Index: 7
#2Urszula Demkow (Medical University of Warsaw)H-Index: 22
Under normal conditions, neutrophils are restricted from trafficking into the brain parenchyma and cerebrospinal fluid by the presence of the brain–blood barrier (BBB). Yet, infiltration of the central nervous system (CNS) by neutrophils is a well-known phenomenon in the course of different pathological conditions, e.g., infection, trauma or neurodegeneration. Different studies have shown that neutrophil products, i.e., free oxygen radicals and proteolytic enzymes, play an important role in the ...
2 CitationsSource
#1Aneta Manda-Handzlik (Medical University of Warsaw)H-Index: 7
#2Weronika Bystrzycka (Medical University of Warsaw)H-Index: 4
Last. Urszula Demkow (Medical University of Warsaw)H-Index: 22
view all 11 authors...
Despite great interest, the mechanism of neutrophil extracellular traps (NETs) release is not fully understood and some aspects of this process, e.g. the role of reactive nitrogen species (RNS), still remain unclear. Therefore, our aim was to investigate the mechanisms underlying RNS-induced formation of NETs and contribution of RNS to NETs release triggered by various physiological and synthetic stimuli. The involvement of RNS in NETs formation was studied in primary human neutrophils and diffe...
2 CitationsSource
#1Aneta Manda-Handzlik (Medical University of Warsaw)H-Index: 7
#2Weronika Bystrzycka (Medical University of Warsaw)H-Index: 4
Last. Olga Ciepiela (Medical University of Warsaw)H-Index: 8
view all 7 authors...
: Studies on neutrophil extracellular traps (NETs) are challenging as neutrophils live shortly and easily become activated. Thus, availability of a cell line model closely resembling the functions of peripheral blood neutrophils would be advantageous. Our purpose was to find a compound that most effectively differentiates human promyelocytic leukemia (HL-60) cells toward granulocyte-like cells able to release NETs. HL-60 cells were differentiated with all-trans retinoic acid (ATRA), dimethyl sul...
2 CitationsSource
Neutrophils are the first line of immune defense against pathogens. They use three major antimicrobial mechanisms: phagocytosis, degranulation, and release of neutrophil extracellular traps (NETs). NETs are structures which consist of nuclear DNA conjugated with antibacterial proteins. They are formed to entrap and kill pathogens. The aim of the study was to evaluate the influence of Escherichia coli (E. coli), Streptococcus pneumoniae (S. pneumoniae), Stenotrophomonas maltophilia (S. maltophili...
1 CitationsSource
Neutrophils are one of the first cells to arrive at the site of infection, where they apply several strategies to kill pathogens: degranulation, respiratory burst, phagocytosis, and release of neutrophil extracellular traps (NETs). Antibiotics have an immunomodulating effect, and they can influence the properties of numerous immune cells, including neutrophils. The aim of this study was to investigate the effects of azithromycin and chloramphenicol on degranulation, apoptosis, respiratory burst,...
4 CitationsSource
1 CitationsSource
Disturbances of the steroidogenesis or altered peripheral metabolism of steroids may result in severe clinical manifestations. Therefore, prompt diagnosis and initiation of medical treatment are desirable. The diagnostics of disorders of steroid hormone production, metabolism, and action have been previously based on immunoassay tests. However, in a modern medical laboratory, due to low accuracy of immunoassays, this technique is continuously replaced by chromatographic separation methods couple...
Source
#1Aneta Manda-Handzlik (Medical University of Warsaw)H-Index: 7
#2Krystyna Sztefko (Jagiellonian University)H-Index: 18
Last. Przemysław J. Tomasik (Jagiellonian University)H-Index: 10
view all 5 authors...
Abstract Objectives: The aim of this study was to establish reference intervals for urine sediment in newborns and infants in the second month of life for the UriSed automated analyser and for bright field microscopy. We also aimed to provide an optimal protocol for UriSed analysis, which best corresponds to the results of manual microscopy. Design and methods: Urine sediment analyses of 75 healthy newborns and infants in the second month of life were performed by manual microscopy and UriSed au...
1 CitationsSource
12