George M Church G M
749Publications
132H-index
74.8kCitations
Publications 749
Newest
Published on Mar 15, 2019in bioRxiv
Caroline J. Smith1
Estimated H-index: 1
(Wyss Institute for Biologically Inspired Engineering),
O. Castanon (Wyss Institute for Biologically Inspired Engineering)+ 9 AuthorsGeorge M Church G M132
Estimated H-index: 132
(Harvard University)
To extend the frontier of genome editing and enable the radical redesign of mammalian genomes, we developed a set of dead-Cas9 base editor (dBEs) variants that allow editing at tens of thousands of loci per cell by overcoming the cell death associated with DNA double-strand breaks (DSBs) and single-strand breaks (SSBs). We used a set of gRNAs targeting repetitive elements - ranging in target copy number from about 31 to 124,000 per cell. dBEs enabled survival after large-scale base editing, allo...
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Published on Mar 8, 2019in Genome Research 10.10
Kornel Labun1
Estimated H-index: 1
(University of Bergen),
Xiaoge Guo (Wyss Institute for Biologically Inspired Engineering)+ 3 AuthorsEivind Valen27
Estimated H-index: 27
(University of Bergen)
We present ampliCan, an analysis tool that unites identification, quantification and visualization of genuine genome editing events from CRISPR amplicon sequencing data. ampliCan overcomes methodological challenges suffered by other tools to estimate the true mutational efficiency in a high-throughput automated fashion. ampliCan controls for biases at every step of the analysis and generates reports that allow users to quickly identify successful editing events or potential issues with their exp...
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Published on Mar 17, 2019in bioRxiv
Shilpa Garg (Harvard University), John Aach30
Estimated H-index: 30
(Harvard University)
+ 2 AuthorsGeorge M Church G M132
Estimated H-index: 132
(Harvard University)
Motivation: Reconstructing high-quality haplotype-resolved assemblies for related individuals of various species has important applications in understanding Mendelian diseases along with evolutionary and comparative genomics. Through major genomics sequencing efforts such as the Personal Genome Project, the Vertebrate Genome Project (VGP), the Earth Biogenome Project (EBP) and the Genome in a Bottle project (GIAB), a variety of sequencing datasets from mother-father-child trios of various diploi...
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Published on Jan 1, 2019in Materials Today 24.54
Tao Wan1
Estimated H-index: 1
(Zhejiang University),
Dong Niu3
Estimated H-index: 3
(Zhejiang University)
+ 3 AuthorsYuan Ping1
Estimated H-index: 1
(Zhejiang University)
Abstract Clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein (CRISPR/Cas) system is an adaptable immune mechanism used by many bacteria to protect themselves from invading nucleic acids, and it has been recently exploited as an efficient tool for site-specific, programmable genome editing in both single cells and whole organisms with a precise manner. CRISPR/Cas system has been shown its great potentials for a wide range of biomedical applications, such as transc...
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Published on Jan 1, 2019in Cell Reports 8.03
Katharina Meyer1
Estimated H-index: 1
(Harvard University),
Heather M. Feldman1
Estimated H-index: 1
(Harvard University)
+ 11 AuthorsYuan-Ta Lin4
Estimated H-index: 4
(Picower Institute for Learning and Memory)
Summary The molecular basis of the earliest neuronal changes that lead to Alzheimer’s disease (AD) is unclear. Here, we analyze neural cells derived from sporadic AD (SAD), APOE4 gene-edited and control induced pluripotent stem cells (iPSCs). We observe major differences in iPSC-derived neural progenitor (NP) cells and neurons in gene networks related to neuronal differentiation, neurogenesis, and synaptic transmission. The iPSC-derived neural cells from SAD patients exhibit accelerated neural d...
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Published on Mar 26, 2019in bioRxiv
Ethan C Alley (Wyss Institute for Biologically Inspired Engineering), Grigory Khimulya + 2 AuthorsGeorge M Church G M132
Estimated H-index: 132
(Wyss Institute for Biologically Inspired Engineering)
Rational protein engineering requires a holistic understanding of protein function. Here, we apply deep learning to unlabelled amino acid sequences to distill the fundamental features of a protein into a statistical representation that is semantically rich and structurally, evolutionarily, and biophysically grounded. We show that the simplest models built on top of this unified representation (UniRep) are broadly applicable and generalize to unseen regions of sequence space. Our data-driven appr...
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Published on Feb 1, 2019in Rejuvenation Research 3.22
Brian P. Hanley , William Bains4
Estimated H-index: 4
,
George M Church G M132
Estimated H-index: 132
(Harvard University)
Abstract We examine self-experimentation ethics history and practice, related law, use scenarios in universities and industry, and attitudes. We show through analysis of the historical development of medical ethics and regulation, from Hippocrates through Good Clinical Practice that there are no ethical barriers to self-experimentation. When the self-experimenter is a true investigator, there is no other party to be protected from unethical behavior. We discuss the n-of-1 issue in self-experimen...
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Published on Jan 30, 2019in Nucleic Acids Research 11.56
Margret R. Hoehe34
Estimated H-index: 34
(Max Planck Society),
Ralf Herwig40
Estimated H-index: 40
(Max Planck Society)
+ 4 AuthorsThomas Huebsch4
Estimated H-index: 4
(Max Planck Society)
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Published on Feb 22, 2019in bioRxiv
Dionis Minev1
Estimated H-index: 1
(Harvard University),
Richard Guerra1
Estimated H-index: 1
(Harvard University)
+ 10 AuthorsWilliam M. Shih35
Estimated H-index: 35
(Harvard University)
We present a rapid, scalable, user-friendly method for in vitro production of high-purity single-stranded DNA (ssDNA) ranging from 89 to 3315 nucleotides in length. PCR with a forward primer bearing a methanol-responsive polymer generates a tagged amplicon that enables selective precipitation of the modified strand under denaturing conditions. We demonstrate that the recovered ssDNA can be used for CRISPR/Cas9 homology-directed repair in human cells, DNA-origami folding, and fluorescent in situ ...
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