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Riffat Ahmed
Oregon National Primate Research Center
Induced pluripotent stem cellMitochondrial DNAMutationGeneticsBiology
15Publications
10H-index
997Citations
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Publications 15
Newest
#1Hong Ma (Oregon National Primate Research Center)H-Index: 27
#2Tomonari Hayama (Oregon National Primate Research Center)H-Index: 8
Last. Shoukhrat Mitalipov (Oregon National Primate Research Center)H-Index: 36
view all 21 authors...
Heritable mitochondrial DNA (mtDNA) mutations are common, yet only a few recurring pathogenic mtDNA variants account for the majority of known familial cases in humans. Purifying selection in the female germline is thought to be responsible for the elimination of most harmful mtDNA mutations during oogenesis. Here we show that deleterious mtDNA mutations are abundant in ovulated mature mouse oocytes and preimplantation embryos recovered from PolG mutator females but not in their live offspring. ...
Source
#1Eunju Kang (Oregon National Primate Research Center)H-Index: 13
#2Jun Wu (Salk Institute for Biological Studies)H-Index: 29
Last. Shoukhrat MitalipovH-Index: 36
view all 30 authors...
Change history In this Letter, there are several errors regarding the assignments of mtDNA haplotypes for a subset of egg donors from our study. These errors have not been corrected online.
2 CitationsSource
#1Hong MaH-Index: 27
Last. Shoukhrat MitalipovH-Index: 36
view all 30 authors...
Source
#1Hong Ma (Oregon National Primate Research Center)H-Index: 27
#2Yeonmi Lee (Oregon National Primate Research Center)H-Index: 6
Last. Shoukhrat Mitalipov (Oregon National Primate Research Center)H-Index: 36
view all 15 authors...
The accumulation of acquired mitochondrial genome (mtDNA) mutations with aging in somatic cells has been implicated in mitochondrial dysfunction and linked to age-onset diseases in humans. Here, we asked if somatic mtDNA mutations are also associated with aging in the mouse. MtDNA integrity in multiple organs and tissues in young and old (2–34 months) wild type (wt) mice was investigated by whole genome sequencing. Remarkably, no acquired somatic mutations were detected in tested tissues. Howeve...
1 CitationsSource
#1Hong Ma (Oregon National Primate Research Center)H-Index: 27
#2Nuria Marti-Gutierrez (OHSU: Oregon Health & Science University)H-Index: 1
Last. Shoukhrat MitalipovH-Index: 36
view all 31 authors...
CRISPR–Cas9 genome editing is used to induce a DNA repair response and correct a disease-causing heterozygous mutation in human embryos with reduced mosaicism and preferential repair using the wild-type copy of the gene.
328 CitationsSource
#1Hong Ma (OHSU: Oregon Health & Science University)H-Index: 27
#2Ryan C. O’Neil (UCSD: University of California, San Diego)H-Index: 5
Last. Shoukhrat Mitalipov (OHSU: Oregon Health & Science University)H-Index: 36
view all 26 authors...
Summary Oocyte defects lie at the heart of some forms of infertility and could potentially be addressed therapeutically by alternative routes for oocyte formation. Here, we describe the generation of functional human oocytes following nuclear transfer of first polar body (PB1) genomes from metaphase II (MII) oocytes into enucleated donor MII cytoplasm (PBNT). The reconstructed oocytes supported the formation of de novo meiotic spindles and, after fertilization with sperm, meiosis completion and ...
25 CitationsSource
#1Eunju Kang (UOU: University of Ulsan)H-Index: 13
#2Jun Wu (Salk Institute for Biological Studies)H-Index: 29
Last. Shoukhrat MitalipovH-Index: 36
view all 30 authors...
Analysis of mitochondrial replacement therapy shows, even with efficient mutant mitochondrial DNA replacement and maintenance in embryonic stem cells, a gradual loss of donor mitochondrial DNA in some lines owing to a polymorphism in the D-loop, potentially causing preferential replication of specific mitochondrial DNA haplotypes.
89 CitationsSource
#1Eunju Kang (Oregon National Primate Research Center)H-Index: 13
#2Xinjian Wang (Cincinnati Children's Hospital Medical Center)H-Index: 20
Last. Shoukhrat MitalipovH-Index: 36
view all 23 authors...
Summary The genetic integrity of iPSCs is an important consideration for therapeutic application. In this study, we examine the accumulation of somatic mitochondrial genome (mtDNA) mutations in skin fibroblasts, blood, and iPSCs derived from young and elderly subjects (24–72 years). We found that pooled skin and blood mtDNA contained low heteroplasmic point mutations, but a panel of ten individual iPSC lines from each tissue or clonally expanded fibroblasts carried an elevated load of heteroplas...
87 CitationsSource
#1Amy Koski (OHSU: Oregon Health & Science University)H-Index: 5
#2Hong Ma (OHSU: Oregon Health & Science University)H-Index: 27
Last. Shoukhrat Mitalipov (OHSU: Oregon Health & Science University)H-Index: 36
view all 26 authors...
Source
#1Hong Ma (Oregon National Primate Research Center)H-Index: 27
#2Clifford D.L. Folmes (Mayo Clinic)H-Index: 18
Last. Shoukhrat Mitalipov (OHSU: Oregon Health & Science University)H-Index: 36
view all 26 authors...
Mitochondria have a major role in energy production via oxidative phosphorylation, which is dependent on the expression of critical genes encoded by mitochondrial (mt)DNA. Mutations in mtDNA can cause fatal or severely debilitating disorders with limited treatment options. Clinical manifestations vary based on mutation type and heteroplasmy (that is, the relative levels of mutant and wild-type mtDNA within each cell). Here we generated genetically corrected pluripotent stem cells (PSCs) from pat...
81 CitationsSource
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