Riffat Ahmed
Oregon National Primate Research Center
14Publications
10H-index
807Citations
Publications 14
Newest
Published on Mar 1, 2019in Nature 41.58
Eunju Kang13
Estimated H-index: 13
(Oregon National Primate Research Center),
Jun Wu28
Estimated H-index: 28
(Salk Institute for Biological Studies)
+ 27 AuthorsYeonmi Lee6
Estimated H-index: 6
(Oregon National Primate Research Center)
Change history In this Letter, there are several errors regarding the assignments of mtDNA haplotypes for a subset of egg donors from our study. These errors have not been corrected online.
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Published on Jul 24, 2018in PLOS ONE 2.77
Hong Ma27
Estimated H-index: 27
(Oregon National Primate Research Center),
Yeonmi Lee6
Estimated H-index: 6
(Oregon National Primate Research Center)
+ 12 AuthorsHayley Darby1
Estimated H-index: 1
(Oregon National Primate Research Center)
The accumulation of acquired mitochondrial genome (mtDNA) mutations with aging in somatic cells has been implicated in mitochondrial dysfunction and linked to age-onset diseases in humans. Here, we asked if somatic mtDNA mutations are also associated with aging in the mouse. MtDNA integrity in multiple organs and tissues in young and old (2–34 months) wild type (wt) mice was investigated by whole genome sequencing. Remarkably, no acquired somatic mutations were detected in tested tissues. Howeve...
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Published on Jan 1, 2017in Cell Stem Cell 23.29
Hong Ma27
Estimated H-index: 27
(Oregon Health & Science University),
Ryan C. O’Neil4
Estimated H-index: 4
(University of California, San Diego)
+ 23 AuthorsYeonmi Lee6
Estimated H-index: 6
(Oregon Health & Science University)
Summary Oocyte defects lie at the heart of some forms of infertility and could potentially be addressed therapeutically by alternative routes for oocyte formation. Here, we describe the generation of functional human oocytes following nuclear transfer of first polar body (PB1) genomes from metaphase II (MII) oocytes into enucleated donor MII cytoplasm (PBNT). The reconstructed oocytes supported the formation of de novo meiotic spindles and, after fertilization with sperm, meiosis completion and ...
18 Citations Source Cite
Published on Aug 1, 2017in Nature 41.58
Hong Ma27
Estimated H-index: 27
(Oregon National Primate Research Center),
Nuria Marti-Gutierrez1
Estimated H-index: 1
(Oregon Health & Science University)
+ 28 AuthorsRiffat Ahmed10
Estimated H-index: 10
(Oregon Health & Science University)
Genome editing could be applied to correct disease-causing mutations in human embryos, but concerns about efficacy and safety are paramount. Shoukhrat Mitalipov and colleagues use CRISPRCas9 to correct a heritable cardiomyopathy mutation in human embryos. By optimizing the experimental conditions, the authors show very reduced mosaicism, and report that for this heterozygous mutation, CRISPRCas9-induced breaks seem to be preferentially repaired using the wild-type allele as a template in human e...
238 Citations Source Cite
Published on May 1, 2016in Cell Stem Cell 23.29
Eunju Kang13
Estimated H-index: 13
(Oregon National Primate Research Center),
Xinjian Wang3
Estimated H-index: 3
(Cincinnati Children's Hospital Medical Center)
+ 20 AuthorsYing Li14
Estimated H-index: 14
(Oregon National Primate Research Center)
Summary The genetic integrity of iPSCs is an important consideration for therapeutic application. In this study, we examine the accumulation of somatic mitochondrial genome (mtDNA) mutations in skin fibroblasts, blood, and iPSCs derived from young and elderly subjects (24–72 years). We found that pooled skin and blood mtDNA contained low heteroplasmic point mutations, but a panel of ten individual iPSC lines from each tissue or clonally expanded fibroblasts carried an elevated load of heteroplas...
70 Citations Source Cite
Published on Nov 30, 2016in Nature 41.58
Eunju Kang13
Estimated H-index: 13
(University of Ulsan),
Jun Wu28
Estimated H-index: 28
(Salk Institute for Biological Studies)
+ 27 AuthorsYeonmi Lee6
Estimated H-index: 6
(Oregon Health & Science University)
Analysis of mitochondrial replacement therapy shows, even with efficient mutant mitochondrial DNA replacement and maintenance in embryonic stem cells, a gradual loss of donor mitochondrial DNA in some lines owing to a polymorphism in the D-loop, potentially causing preferential replication of specific mitochondrial DNA haplotypes.
59 Citations Source Cite
Published on Sep 1, 2015in Mitochondrion 3.23
Amy Koski5
Estimated H-index: 5
(Oregon Health & Science University),
Hong Ma27
Estimated H-index: 27
(Oregon Health & Science University)
+ 23 AuthorsXinjian Wang17
Estimated H-index: 17
(Cincinnati Children's Hospital Medical Center)
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Published on Aug 1, 2015in Nature 41.58
Hong Ma27
Estimated H-index: 27
(Oregon National Primate Research Center),
Clifford D.L. Folmes16
Estimated H-index: 16
(Mayo Clinic)
+ 23 AuthorsRiffat Ahmed10
Estimated H-index: 10
(Oregon Health & Science University)
Mutations in mitochondrial (mt)DNA are associated with severe disorders for which treatment is currently limited; this study shows that mtDNA mutations can be genetically corrected and normal metabolic function restored in cells derived from patients with mtDNA disease and reprogrammed to pluripotency through factor-mediated reprogramming or via a somatic cell nuclear transfer approach.
75 Citations Source Cite
Published on Jul 1, 2014in Nature 41.58
Hong Ma27
Estimated H-index: 27
(Oregon National Primate Research Center),
Robert Morey9
Estimated H-index: 9
(University of California, San Diego)
+ 23 AuthorsKaren Sabatini6
Estimated H-index: 6
(University of California, San Diego)
Genome-wide analysis of matched human IVF embryonic stem cells (IVF ES cells), induced pluripotent stem cells (iPS cells) and nuclear transfer ES cells (NT ES cells) derived by somatic cell nuclear transfer (SCNT) reveals that human somatic cells can be faithfully reprogrammed to pluripotency by SCNT; NT ES cells and iPS cells derived from the same somatic cells contain comparable numbers of de novo copy number variations, but whereas DNA methylation and transcriptome profiles of NT ES cells and...
184 Citations Source Cite
Published on Apr 2, 2013in PLOS ONE 2.77
Caroline A. Enns35
Estimated H-index: 35
,
Riffat Ahmed10
Estimated H-index: 10
(Oregon Health & Science University)
+ 4 AuthorsAn Sheng Zhang12
Estimated H-index: 12
Bone morphogenetic protein 6 (BMP6) is an essential cytokine for the expression of hepcidin, an iron regulatory hormone secreted predominantly by hepatocytes. Bmp6 expression is upregulated by increased iron-levels in the liver. Both hepatocytes and non-parenchymal liver cells have detectable Bmp6 mRNA. Here we showed that induction of hepcidin expression in hepatocytes by dietary iron is associated with an elevation of Bmp6 mRNA in the non-parenchymal cells of the liver. Consistently, incubatio...
33 Citations Source Cite
12